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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/m_MTBLS15140_GC-MS_positive_high-polarity_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/a_MTBLS15140_GC-MS_positive_high-polarity.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/s_MTBLS15140.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/FILES/RAW_FILES/DMSO_2.D.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/FILES/RAW_FILES/Q5_2.D.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/FILES/RAW_FILES/DMSO_3.D.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/FILES/RAW_FILES/Q5_3.D.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/FILES/RAW_FILES/Q5_1.D.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140/FILES/RAW_FILES/DMSO_1.D.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15140Metabolite identification was performed by matching mass spectra and retention indices against public and commercial mass spectral libraries. The FiehnLib mass spectral library and the NIST (National Institute of Standards and Technology) library were used for compound identification. Identification criteria were based on mass spectral matching score and retention index match. Compounds with a similarity score above the default threshold of the respective library were considered as putative identifications. Additionally, compounds were cross-referenced against the KEGG (Kyoto Encyclopedia of Genes and Genomes) database for pathway annotation. The identification and annotation process was performed by Shanghai BIOTREE Biological Technology Co., Ltd.MetaboLightsPublicGas Chromatography MS - positive - high-polarityGas chromatography (GC) separation was performed on an Agilent 7890A GC system equipped with a DB-WAX capillary column (Agilent 122-7032, 30 m × 0.25 mm I.D., 0.25 µm film thickness). Helium was used as the carrier gas at a constant flow rate of 1.0 mL/min. The injection was performed in splitless mode at 250 °C with a purge flow of 20 mL/min at 0.75 min. The oven temperature program was as follows: initial temperature 40 °C held for 4 min, then ramped at 5 °C/min to 245 °C and held for 5 min. The total run time was 50 min. The inlet liner used was an Agilent 19251-60540 (split, straight, with wool). The GC was coupled to a mass spectrometer via a transfer line heated at 250 °C.Analysis of Volatile Organic Compounds (VOCs) in Streptomyces sp. 30177 treated with C8-HSL using Headspace Solid-Phase Microextraction (HS-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS).South China Normal Universityqin xiaoStreptomyces sp. 30177mass spectrometry assayVolatile organic compounds (VOCs) were extracted from the headspace of bacterial cultures using solid-phase microextraction (SPME). A DVB/PDMS (divinylbenzene/polydimethylsiloxane) SPME fiber (5610-5874, 0.01 m fiber length) was used for extraction. The fiber was conditioned at 250 °C for 10 min prior to use. Each sample vial was incubated at 60 °C for 15 min with agitation (250 rpm) using a PAL3 autosampler agitator, followed by headspace extraction at 60 °C for 30 min with agitation. After extraction, the fiber was inserted into the GC inlet and desorbed at 250 °C for 4 min. Solvent blank samples and quality control (QC) samples (pooled extracts) were prepared and analyzed under the same conditions to monitor system performance and ensure data quality.Streptomyces sp. 30177https://www.ebi.ac.uk/metabolights/MTBLS15140Dan Wang. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. Guangzhou, Guangdong Province, China. wangdanzh@gdaas.cn.Wenjie Gu. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. Guangzhou, Guangdong Province, China. guwenjie0818@163.com.Raw GC-MS data were processed by Shanghai BIOTREE Biological Technology Co., Ltd. The raw data files (Agilent .D format) underwent peak detection, deconvolution, alignment, and compound identification. Compound identification was performed by matching mass spectra and retention indices against the FiehnLib mass spectral library and the NIST library. Peak areas were normalized by total ion current (TIC). Missing values were filtered and imputed using half of the minimum value. Statistical analysis and visualization were performed using R software (version 3.6.3 / 3.3.5, R Foundation for Statistical Computing, Vienna, Austria) with relevant packages, including ggplot2, pheatmap, corrplot, and fmsb. Multivariate analysis (PCA and OPLS-DA) was performed using SIMCA software (version 18.0.1, Sartorius Stedim Data Analytics AB, Umea, Sweden).Treatment19854815383@163.comC8-HSL was dissolved in DMSO to prepare a 2 mM stock solution, which was then mixed with S. fodineus 30177 spores to a final concentration of 20 µM. DMSO mixed with S. fodineus 30177 spores was used as a control. After 5 days of cultivation, the bacterial cells were collected for further analysis.MetabolomicsMetabolomicsuntargeted analysisC8-HSLAgilent 5977B MSDStreptomyces sp.30177Volatile Organic Compounds (VOCs)Agilent 6890 GCexperimental blankStreptomyces sp. 30177Metabolomicsuntargeted analysisAgilent 5977B MSDC8-HSLStreptomyces sp.30177Agilent 6890 GCVolatile Organic Compounds (VOCs)experimental blankStreptomyces sp. 30177Mass spectrometry detection was performed on an Agilent 5977B mass spectrometer (or Agilent 5977A MSD) coupled to the GC system. Electron ionization (EI) was used as the ion source at 230 °C, with the quadrupole temperature maintained at 150 °C. The mass spectrometer was operated in full scan mode with a scan range of m/z 20–400. The ionization energy was 70 eV. Data acquisition and processing were performed using Agilent MassHunter software. The transfer line temperature was set to 250 °C.falseAnalysis of Volatile Organic Compounds (VOCs) in Streptomyces sp. 30177 treated with C8-HSL using Headspace Solid-Phase Microextraction (HS-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS)This study analyzed the volatile organic compounds (VOCs) produced by Streptomyces sp. 30177 in response to C8-HSL treatment. C8-HSL was dissolved and added to the culture of Streptomyces sp. 30177. After incubation, the headspace VOCs were extracted using solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). The raw data were acquired and processed using Agilent MassHunter software. This study aims to reveal the impact of C8-HSL on the volatile organic compound profile of Streptomyces sp. 30177.2026-07-242026-07-23MTBLS15140