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/pub/databases/metabolights/studies/public/MTBLS15146/FILES/RAW_FILES/NEG/NEG_MKG1705242_QC03.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15146/FILES/RAW_FILES/POS/POS_MKG1705242_QC01.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15146/FILES/RAW_FILES/POS/POS_MKG1705255_S_61.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15146/FILES/RAW_FILES/NEG/NEG_MKG1705253_T_8.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15146/FILES/RAW_FILES/NEG/NEG_MKG1705251_T_32.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15146<p>Subsequently, the software was employed for characteristic peak search and library identification by matching MS and MS/MS mass spectrometry data with a metabolite database. The MS mass error was set to less than 10 ppm, and metabolites were identified based on the matching scores from the secondary mass spectrum analysis.The primary databases include mainstream public databases such as http://www.hmdb.ca/ and https://metlin.scripps.edu/, as well as self-developed databases.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic conditions: The chromatographic column used was an ACQUITY UPLC HSS T3 (100 mm × 2.1 mm inner diameter, 1.8 µm; Waters, Milford, USA); mobile phase A consisted of 95% water + 5% acetonitrile (containing 0.1% formic acid), and mobile phase B consisted of 47.5% acetonitrile + 47.5% isopropanol + 5% water (containing 0.1% formic acid); the injection volume was 3 μL, and the column temperature was maintained at 40°C.</p>Effects of Different Nutritional Levels of Diets on Serum Metabolomics Profiles in Postpartum Tan Sheep.Northwest Agriculture & Forestry UniversityLele Zhubloodmass spectrometry assay<p>Blood was collected from the jugular vein, left at room temperature for 2 hours, and serum was separated by centrifugation at 3000 rpm for 10 minutes. A sample of 100 μL of rumen fluid or serum was added to 400 μL of pre-cooled acetonitrile-methanol extraction solution (1:1, v/v, containing an internal standard). After vortexing, low-temperature sonication, protein precipitation, and centrifugation, the supernatant was collected. The samples were dried under nitrogen and resolventized in acetonitrile-water (1:1, v/v) for UHPLC-MS/MS analysis. Equal volumes of all samples were mixed to prepare quality control samples, with one quality control sample added to every 5–10 test samples during analysis.</p>sheephttps://www.ebi.ac.uk/metabolights/MTBLS15146Lele Zhu. Northwest Agriculture & Forestry University. 3514953885@qq.com.<p>The raw data were imported into the metabolomics analysis software Progenesis QI v3.0 (Waters Corporation, Milford, USA) for baseline filtering, peak identification, integration, retention time calibration, and peak alignment, ultimately yielding a data matrix containing information such as retention times, mass-to-charge ratios, and peak intensities.</p>Treatment3514953885@qq.com<p>The sheep were divided into three groups: the low-nutrient group (LND), the medium-nutrient group (MND), and the high-nutrient group (HND).</p>MetabolomicsMetabolomicsuntargeted analysisbloodmethanogenesisexperimental sampledietary nutrient supplyacetate metabolismThermo Scientific Vanquish UHPLC Systemreproductive efficiencyThermo Scientific Q Exactive HF-XRumen microbiomecarbon allocationsheepMetabolomicsuntargeted analysisbloodmethanogenesisexperimental sampledietary nutrient supplyThermo Scientific Vanquish UHPLC Systemacetate metabolismreproductive efficiencyThermo Scientific Q Exactive HF-XRumen microbiomecarbon allocationsheep<p>Metabolite detection was performed using the UHPLC-Q Exactive HF-X system with Waters HSS T3 chromatographic columns. Mass spectrometry signals were collected in both positive and negative ion modes, with a scanning range of m/z 70–1050. Raw data were processed with Progenesis QI for peak identification, peak integration, retention time calibration, and peak alignment. Metabolites were identified by matching MS and MS/MS data against HMDB, Metlin, and proprietary databases 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of Different Nutritional Levels of Diets on Serum Metabolomics Profiles in Postpartum Tan SheepThis study investigated the metabolomic characteristics of rumen fluid in postpartum New Zealand ewes after they were fed diets that met 100%, 115% or 130% of their nutritional requirements. Six ewes in each group collected individual blood samples at the time of their first estrus.2026-07-252026-07-24MTBLS15146