MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/m_MTBLS15156_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/m_MTBLS15156_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/s_MTBLS15156.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/a_MTBLS15156_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/a_MTBLS15156_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_FLO8_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_ATCC2001_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_QC03.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_FLO8_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_ATCC2001_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_QC03.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_QC02.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_ATCC2001_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_FLO8_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_FLO8_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_FLO8_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_FLO8_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_ATCC2001_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_QC02.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_ATCC2001_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_FLO8_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_ATCC2001_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_QC01.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_QC01.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_FLO8_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_ATCC2001_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_FLO8_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_FLO8_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_ATCC2001_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_FLO8_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_ATCC2001_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_ATCC2001_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/POS_ATCC2001_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_FLO8_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156/FILES/RAW_FILES/NEG_ATCC2001_4.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15156<p>Metabolites were annotated by matching MS and MS/MS spectra against public databases, including HMDB and METLIN, together with an in-house spectral library. Metabolite identification was performed using a mass tolerance of &lt;10 ppm and MS/MS spectral similarity scores.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using an ACQUITY UPLC HSS T3 column (100 × 2.1 mm, 1.8 μm; Waters) on a Thermo Scientific Vanquish Horizon UHPLC system. The mobile phase consisted of solvent A (95% water and 5% acetonitrile containing 0.1% formic acid) and solvent B (47.5% acetonitrile, 47.5% isopropanol, and 5% water containing 0.1% formic acid). The column temperature was maintained at 40°C, and the injection volume was 3 μL.</p>Flo8 regulates iron homeostasis through PLPHP-mediated vitamin B6 homeostasis in Candida glabrata.Ruijin Hospital, Shanghai Jiao Tong University School of Medicinewang lulingCell Pelletmass spectrometry assay<p>Samples were transferred into 2 mL centrifuge tubes containing a 6 mm grinding bead. Metabolites were extracted with 500 μL of methanol/water (4:1, v/v) containing internal standards. Samples were homogenized at −10°C for 6 min (50 Hz), followed by ultrasonic extraction at 5°C for 30 min. After incubation at −20°C for 30 min, samples were centrifuged at 13,000 × g for 15 min at 4°C, and the supernatants were transferred into autosampler vials for LC–MS analysis. QC samples were prepared by pooling 20 μL of supernatant from each sample.&nbsp;</p>Candida glabratahttps://www.ebi.ac.uk/metabolights/MTBLS15156wang luling. Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. wangluling63@outlook.com.<p>Raw LC–MS data were processed using Progenesis QI v3.0 (Waters Corporation, Milford, MA, USA). Data processing included baseline correction, peak detection, peak integration, retention time alignment, and feature alignment to generate a data matrix containing retention time, m/z, and peak intensity for downstream analyses.&nbsp;</p>Genetic modificationwangluling63@outlook.com<p>Log-phase Candida glabrata samples were collected and immediately processed for metabolomic analysis. A total of 12 biological samples were included in this study.Equal aliquots of the supernatant from each sample were pooled to prepare quality control (QC) samples for monitoring analytical stability throughout the LC–MS analysis.&nbsp;</p>Metabolomicspooled quality control sampleMetabolomicsVanquish Horizonuntargeted analysisCandida glabratametabolomics investigationgenetic modificationThermo Scientific Q Exactive HFdata-dependent acquisitionCell Pelletexperimental sampleuntargeted metabolite profilingpooled quality control sampleMetabolomicsVanquish Horizonuntargeted analysisCandida glabratametabolomics investigationgenetic modificationThermo Scientific Q Exactive HFdata-dependent acquisitionCell Pelletexperimental sampleuntargeted metabolite profiling<p>Mass spectrometric analysis was performed on a Q Exactive HF-X Orbitrap mass spectrometer (Thermo Scientific) equipped with an electrospray ionization (ESI) source operating in both positive and negative ion modes. Full-scan data were acquired over an m/z range of 70–1050. Instrument parameters included a sheath gas flow rate of 50 arb, auxiliary gas flow rate of 13 arb, heater temperature of 425°C, capillary temperature of 325°C, spray voltages of +3.5 kV and −3.5 kV, S-Lens RF level of 50, normalized collision energies of 20%, 40%, and 60%, and resolutions of 60,000 for Full MS and 7,500 for MS/MS acquisition.</p>PenconazoleSulfanilic AcidGardnutine9H-Purine-9-OlArgininosuccinic AcidCaprolactamDipropylene Glycol Dimethyl EtherGenipic AcidPc(34:2)ProlylprolineN,N-Dimethyltetradecylamine-N-OxideOleyl Sarcosine2,3-DiaminopyridineDeoxycholic AcidL-Histidine1,2-Dimyristoleoyl-Sn-Glycero-3-PhosphocholineProlyl-AsparagineN-(4-Hydroxycinnamoyl)TyrosineCitrullineErucoylacetoneAsn-Ala-Asp1,5-Naphthalenediamine6-Hydroxyhexanoic Acid(+/-)-(Z)-2-(5-Tetradecenyl)CyclobutanoneN-Methyl-N'-NitrosoguanidineDelta-ValerobetaineArenarine D4-HydroxybenzaldehydeLimonene-1,2-EpoxideFurfuralLauryl Diethanolamide(2E,8E)-Piperamide-C9:2PilocarpineProstaglandin E37-Methylpyrimido[4,5-D]Pyrimidin-4-Amine2-Methyl-3,5-Dihydro-2H-1,5-Benzoxazepin-4-One(-)-Medicarpin-3-O-GlucosideSoyasaponin Bb4-MethylcatecholL-Theanine6-MethylquinolineN-CyclohexanecarbonylpentadecylamineGlutaric AcidAdenosine Monophosphate5-Butyl-6-(Hydroxymethyl)-4-Methoxypyran-2-One6-Methylmercaptopurine4-MethylstyreneIsophoroneN-Methyl-L-Glutamic AcidOleamidePro-GlyCinnamic AcidVindolineN-Eicosapentaenoyl GlycineAcetoacetanilideAdp4-(4-Hydroxyphenyl)-2-Butanone O-[2,6-Digalloylglucoside]L-OrnithineEtomidateN-BenzyloxycarbonylglycineHexadecanedioic AcidN-(2-Hydroxyethyl)AcetamideGlyceryl PalmitoleateOxaceprol(2S)-2-Amino-5-[(2R)-2-Amino-3-Sulfanylpropanoyl]Oxy-5-Oxopentanoic AcidGentamicin C4-MethoxybenzaldehydeFapy-Adenine3-Pyrimidin-2-Yl-Propionic Acid2,4-QuinolinediolNae(16:0)AnisoleSpb(20:0_3O)Hydroxylated LecithinAsn-ValIndoleContignasterolProstaglandin A2Prostaglandin A1Benzamide6-Amino-N-[6-Keto-6-(6-Ketohexylamino)Hexyl]Hexanamide12-Aminododecanoic Acid4-MethylpyrimidineIle-Glu2-(4-Benzylpiperazine-1-Carbonyl)-5-Propylpyrazolo[1,5-A]Quinoxalin-4-OneTyr-GlyGamma-GlutamylisoleucineVenlafaxineN-(2-Furoyl)Glycine4'-O-Methylkanzonol WIle-GlyAdenosineSecnidazole2-Acetyl-4,5-Dihydrothiazole2-(3-Carboxy-3-Aminopropyl)-L-HistidineNadMenthofuranDl-P-Chlorophenylalanine Methyl Ester HydrochlorideS-(2-Carboxypropyl)GlutathioneDeoxyguanylic AcidButyryl Trihexyl Citrate5-Aminopentanoic AcidRiboprineLauramine Oxide(+/-)-FuraneolSpb(14:0_2O)Pro-Pro-Pro3,4,5-Trimethylphenyl MethylcarbamateMethionyl-Cysteine2-Pyridyl(2-Quinolyl)Methanone1,4-Diaminobutane2-[(4-Ethoxyphenyl)Methylene]-3,4-Dihydro-1(2H)-Naphthalenone3-(7'-Methylthio)HeptylmalateBuprenorphineO-XyleneAcetaminophen5-Methyl FurfuralCabergolineL-ProlinamideGamma-D-Glutamylglycine2-(4-Hydroxyphenyl)Naphthalic AnhydridePc(O-16:1)(E)-2-(4-Methylpent-3-Enyl)-6-Oxohept-2-Enoic AcidNeocarzinostatin ChromophoreLysylasparagineGeranyl Acetone1,2-DipalmitoylphosphatidylcholineTrans-3-Indoleacrylic Acid2,5,7,8-Tetramethyl-2-(2'-Carboxyethyl)-6-HydroxychromanAdenosine 5'-DiphosphateMg(18:1(9Z)/0:0/0:0)N-[2-Hydroxy-2-(4-Nitrophenyl)Ethyl]AcetamidePolicapramIndole-3-CarboxaldehydeGlu-Val3-(2,6-Dioxocyclohexyl)Propanenitrile7-MethylguanineN-Acetyl-L-ArginineN-(3-Amino-2-Hydroxy-3-Oxopropyl)-L-ValineCoproporphyrin IiiRimantadineD-(+)-TryptophanL-GlutathioneTanacetol BN-(Carboxyacetyl)-L-TryptophanArachidoyl EthanolamideMethyl D-AlaninateBenzophenonePhosphoenol PyruvateImidazole Lactic AcidLys-ProPro Phe9,12-Octadecadiynoic Acid10-Hydroxydecanoic AcidPc(17:0/0:0)AdrenochromeZ-Llnle-ChoGly-Gln-ProGlutamic AcidBenzofuranVal Thr TrpLys-TyrMuramic AcidPsilostachyinPc(14:1/0:0)Ala-Tyr4-O-Caffeoyl-3-O-Feruloylquinic AcidKapaEstriol 16Alpha-(Beta-D-Glucuronide)3-Hydroxy-3-(2-Pyridylmethyl)Indolin-2-OneGenisteinEthyl (5-(4-Chlorophenyl)-1,3,4-Oxadiazol-2-Yl)Acetate2,4-Thiazolidinedicarboxylic Acid, 2-Methyl-Glu IleSpb(19:0_2O)LunatoneN-[6-[(2-Fluorophenyl)Methylsulfanyl]Pyridazin-3-Yl]Thiophene-2-Carboxamide[(3S,4S,5S,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Oxan-2-Yl] Octadec-9-Enoate2-O-MethyladenosineEthylphthalyl Ethylglycolate3-KetosphingosineThymineO6-Carboxymethyl-2'-Deoxy-Guanosine1-MethyladenosineDeoxycholylarginineColupox B4-HydroxyisoleucineSarcosineMaltolDodecanoic AcidEpothilone DGamma-Glu-LeuZalcitabineGamma-GlutamylcysteineGeldanamycin6-Hydroxy-2-(3-Hydroxy-2,4-Dimethoxyphenyl)-1-Benzofuran-3-CarbaldehydeN,N-DimethylguanosineDibutyl SebacatePro-Leu1-MononitroglycerinL-ThreoninePiperonyl Butoxide4-Amino-3-Phenylbutanoic Acid4-MethylanilinePyridoxalFlavin Adenine Dinucleotide (Fad)Leu-LysLysope(16:1(9Z)/0:0)Tetrahydrothiophene SulfoxideHexylitaconic Acid F1-Oleoyl-2-Myristoyl-Sn-Glycero-3-Phosphocholine1-MethylguanosineLubiminol13-DihydrodaunorubicinN-Octyl-2-PyrrolidoneOctopamineRuscogenin(11R,16S)-MisoprostolTriethanolaminePro-Arg1-Acetyl-Beta-CarbolineN-Nervonoyl TryptophanCytosine Arabinoside MonophosphateUridine Monophosphate (Ump)3-Hydroxy-4-MethoxyxanthoneLevocarnitine8-Amino Caprylic AcidLpc(12:0-Sn1)DhurrinLpc(17:1-Sn1)6-Ethylchenodeoxycholic AcidPalmitoyl SerinolTyr-AlaL-ValineUracilVidarabineGpcho(16:0/14:1)CuminaldehydeGln-Phe-ArgPe(O-16:1/2:0)2-Methylpropanamine3-Methylbenzaldehyde3-Hydroxy-1-Methyl-3-(2-Oxopropyl)Indol-2-OneOctopineLys Leu GlnPe(16:1/16:1)Pro-AlaCer(8:1_2O/3:0)Cytosine1H-Indene-3-CarboxamideDephospho-Coa2-PicolineTriton X-100Gpcho(14:1/14:1)Sm(8:1_2O/17:2)N6-Methyladenosine(13E)-11A-Hydroxy-9,15-Dioxoprost-13-Enoic AcidPhenylalanyl-AlanineValyl-Prolyl-Glycyl-Valyl-Glycine5,7-Megastigmadien-9-Ol Glucoside12-Methyltridecanoic AcidPc(16:1/0:0)Gamma-GlutamylphenylalanineKynurenic AcidSpongouridinCholine PhosphateAllocholic AcidDiallyl Trisulfide3,3'-Thiobispropanoic AcidCrotalineDeoxycarnitine3-Hydroxy-L-Proline4-Methylpyridine-3-Sulfonic AcidN-Lactoyl-Phenylalanine2-(Ethylamino)EthanolAsp-LeuAlpha-Hederin2-(1H-Indol-3-Yl)AcetaldehydeLacosamide9-(4-Fluoro-3-(Hydroxymethyl)Butyl)GuaninePc(32:0)BicinePe(Pgf2Alpha/20:0)2-Morpholin-4-Yl-5-(3-Thiophen-2-Ylprop-2-Enoylamino)Benzoic Acid1-Dodecyl-2-Pyrrolidinone1-[2-(3,5-Dimethoxyphenyl)Propan-2-Yloxycarbonyl]Pyrrolidine-2-Carboxylic AcidP-TolualdehydeGlu Cys LeuN-[3-(Dimethylamino)Propyl]DodecanamideGpcho(18:4/16:1)(R)-1-O-[B-D-Apiofuranosyl-(1->2)-B-D-Glucopyranoside]-1,3-Octanediol2-Hydroxy-4,7-Dimethoxy-2H-1,4-Benzoxazin-3(4H)-OneGly-Val5-Methyl-1H-BenzotriazoleDeoxyadenosinePyridoxal Phosphate5,7Alpha-Dihydro-1,4,4,7A-Tetramethyl-4H-IndeneTaurine GlutamateKetone EsterThr-Asn2-(3-Methyloxan-4-Yl)Ethanol12A-Hydroxydalpanol(E)-1-(4-Methylquinazolin-2(1H)-Ylidene)GuanidineValtrate4-O-P-Coumaroylquinic AcidGlycerophospho-N-Arachidonoyl EthanolamineCysteinylglycineValylhydroxyprolinePc(Pgf1Alpha/14:1(9Z))27-NorcholestanehexolN-Acetyl-L-PhenylalanineSer Leu2-Aminooctanedioic AcidPc(32:2)N-AcetyltyramineThr-LeuCholine GlycerophosphateArg-LeuProtoporphyrin IxHymenoxon7-EthylguanineCyclo[Dha-Unk-Dl-Val-Dl-Xithr]S-HydroxymethylglutathioneN-AcetylputrescineO-AcetylcarnitineCarteolol4-Methyl-2-Phenyl-1H-Pyridazine-3,6-DioneXanthinePlatelet-Activating FactorDihomo-Alpha-Linolenic AcidL-GlutamineCholineS-Ethyl-L-CysteineAldobiouronic Acid D32,3-Dihydrobenzofuran2-Amino-2-Methylpropan-1-Ol4-(4-Methoxyphenyl)-2-(4-Pyridinyl)PyrimidineArg-Phe2R-Hydroxymethyl-3S-HydroxypyrolidineIndoline2-Phenylethyl Beta-D-GlucopyranosideDivinyl SulfideLpc(18:2-Sn1)Heptyl 4-Hydroxybenzoate(S)-5'-Deoxy-5'-(Methylsulfinyl)Adenosine2-AminopurineGly IleGpcho(16:1/16:1)Val-GlyS-AdenosylhomocysteineMethyl L-LeucinateN'-(2-Cyano-3-Fluoro-N-Methylanilino)EthanimidamideAvocadene 2-Acetate5-Amino-2-(Dimethylamino)Benzoic Acid11-(5-Heptyl-3-Methylfuran-2-Yl)UndecanoylcarnitineHeptadecasphinganine1,2,4-NonadecanetriolThr-ProGpcho(16:0/16:1)Epigallocatechin2-PhenylacetohydrazideN,N-Dimethyldecylamine N-Oxide4-Methoxybenzyl O-(2-Sulfoglucoside)N-(3,5-Dimethylpyrazin-2-Yl)BenzenesulfonamideLinoleoyl EthanolamideGlutathione Episulfonium IonUlipristal AcetatePiperidineGemeprost3-(3,4-Dihydroxyphenyl)-N-[2-(3,4-Dihydroxyphenyl)Ethyl]Prop-2-Enamide1-Fluoro-3-(2-Methylphenyl)UracilArmillaramide(10Z)-Nonadec-10-Enoylcarnitine9-Hexadecenoylcholine1-[(2R,3R,4S,5R)-3,4-Dihydroxy-5-(Hydroxymethyl)Oxolan-2-Yl]Oxypyrimidin-2-OneAlfentanilCetylmannosideGlucosylsphingosinePc(15:1/15:1)3-Hydroxy-2,6,6-Trimethylcyclohexene-1-Carboxylic AcidLeu Lys AspSer-Lys6-(4-Methoxyphenyl)Pyrimidine-2,4-DiaminePalustrineRiboflavin1,2-Dioctanoyl PcGpcho(16:0/20:5)N(6)-6(R,S)-Lipoyl-L-LysineEstradiol 17-SulfateGlu-Leu4-PiperidinecarboxamideCichoriin3-Amino-2-Methoxynonadec-5-En-4-OlQuinolineCis-4-HeptenalGlu Cys ValN6,N6,N6-Trimethyl-L-LysineHistidylprolineIndole-3-Acetic AcidChlorobenzeneNorcholic AcidPidotimod6-MethoxyquinolineTyrosylhydroxyproline2,3-Dinor-Txb1Gln-Ile-LysAnisperimusL-ProlinePhenolN-Methylanthranilic AcidDiglycidyl EtherIsoambrettolidePc(18:1/0:0)3-(((4-Chlorophenyl)Sulfonyl)Methyl)-N-HydroxybenzimidamideButyrylcarnitinePantothenic Acid1-Aminocyclopropane-1-CarboxylateN-AcetylsphinganineArg-ValSolutol Hs 15L-Cys-GlyGlycitein4,6-Dihydroxy-3-Methyl-2,3-Dihydroisoindol-1-OneGpcho(18:4/14:1)Blumealactone AL-FormylkynureninePe(O-12:0/4:0)1,9-DimethylguanineDihydrocapsaicin5-(2-Methylpropyl)-3,6-Dioxo-2-Piperazinepropanoic AcidN1-[2-(Methylthio)-6-(Trifluoromethoxy)Phenyl]-2,2-DimethylpropanamideSpb(20:0_2O)Vitamin D2FudosteineN-MethylsphingosineBisisodiospyrinUrobilinogenN-AcetyltryptamineBis-(Acetyl)DiaminopentaneGlucosaminic AcidRollinecin ABeta-Alanyl-L-ArginineN-Alpha-MethylhistamineLys-Ile6-Methylpteridine-2,4-DiamineEpicatechin 3-O-(4-Methylgallate)Spisulosine(2R,3Z)-PhycocyanobilinVulgarole25-HydroxycholecalciferolMidodrine(1(10)E,4A,5E)-1(10),5-Germacradiene-12-Acetoxy-4,11-DiolVal Trp ValFenvalerate2-Methylindole1-MethylguaninePe(19:1(9Z)/0:0)Vitamin B7N-Linoleoyl IsoleucineAcidissiminol Epoxide4-Methyl-5-Thiazoleethanol2-Oxobutanoic AcidN-Arachidonoyl Arginine4-Oxo-2-NonenalIsoindolineDiethanolamineSwainsonineThiamineN,N-DiisopropylethylamineCiticholine4-EthynylanilineAlpha-Methylene-Gamma-ButyrolactonePhe-LysKetoisophorone4,5-Di-O-Caffeoylquinic AcidL-Tyrosine4-Methyl-1H-BenzotriazolePinolenic Acid4-HydroxynicotinamideN6-(Delta2-Isopentenyl)-Adenosine 5'-Monophosphate2-Keto-6-AcetamidocaproateGlu Cys GluTetrahca(2E,4E)-N-(1-Hydroxy-3-Methylpentan-2-Yl)-2-Methylhexa-2,4-DienamideClitocineIsoguanosine5'-Deoxy-5-FluorocytidineSpermidine2-Acetylpyridine2-Hydroxycinnamic Acid2-NaphthylaminePc(O-15:1)Val-ProM-Xylene5-MethylthioriboseLdgts(14:1)Cys Glu(Z)-6-Methyl-2-(4-Methylpent-3-Enyl)Hept-2-Enedioic AcidThr-Ala-Tyr3,7-Dihydropurin-6-One4,4'-DihydroxyantipyrineBeta-IononeArg-GluThr-Ser-IleN-(3-Chloro-4-Methylphenyl)-2-(5-Pyrazin-2-Yl-1,3,4-Oxadiazol-2-Yl)AcetamideOctadecanamideD-Pyroglutamic AcidBeta-D-GlucosamineS-(2-Carboxyethyl)-L-CysteineIle LysZoniporideDocosahexaenoic AcidAstromicinAsp-Ile-Ile3-Ethyl-N-Propan-2-Yl-[1,2,4]Triazolo[4,3-B]Pyridazin-6-AminePyridoxineGuanosine 5'-MonophosphateKeto-3-Deoxy-D-Manno-Octulosonic Acid2-Aminohexanedioic AcidGln-Val2-Aminoheptanedioic Acid5'-Deoxy-5'-MethylthioadenosinePc(15:1/0:0)ZidovudineHistidinalLpc(18:3-Sn1)9H-Pyrido[3,4-B]Indole-1-MethanolCucurbic Acid3-Ketosphinganine2-Methyl-3-Pyrimidin-2-Yl-Propionic AcidAmiloride(1R,3S)-3-(6-Aminopurin-9-Yl)Cyclopentan-1-OlGpetn(17:1/17:1)Glu Cys Ile8-HydroxyadenineBenzoic AldehydePipecolic AcidN-Epsilon-Acetyl-L-Lysine10E-Heptadecenoic AcidPterinAsn-Lys(-)-3-Cyanomethyl-3-Hydroxy-1H-Indol-2(3H)-OneGrayanotoxin4-(Methoxymethyl)-1,3-Dimethyl-1H-Pyrazolo[3,4-B]Pyridin-6-Ol7E-Mycosinyl AcetateNadhGeranyl AcetateTryptopholCorchorifatty Acid DL-Lysine1-Methoxy-2-Propylamine1-Phenylpropane-1,2-DioneCys-GlyBenzyl AlcoholSulfamethoxazole N1-Glucuronide(+/-)-Propionylcarnitine4-Dodecan-6-Ylbenzenesulfonic AcidPeg N7Arg-IleSaphenic Acid Methyl EsterVal-Leu3-Penten-2-OlSphinganineNicotinic Acid MononucleotideCer(8:1_2O/5:0)Citiolone(3S,8As)-3-[(1R)-1-Hydroxyethyl]Hexahydropyrrolo[1,2-A]Pyrazine-1,4-DionePhe SerLpc(16:2-Sn1)Gamma-GlutamylfelinylglycineCamphorGpcho(15:1/18:4)VelvionePhe AspKioukasin A2'-O-MethylphaseollinisoflavanEphedrine1-Anilino-9,10-Dioxo-2-Anthroic AcidAcexamic AcidTrans-Zeatin Riboside Monophosphate5-HydroxytryptophanEthyl 2-(Tert-Butyl)-3-Chloro-5-Cyano-6-HydroxyisonicotinateN-([1,2,4]Triazolo[4,3-A]Pyridin-8-Yl)Pyrazine-2-Carboxamide3''-O-AcetylafzelinN-(2-Phenylethyl)Acetamide11-Aminoundecanoic Acid3-GonalN-Acetyl-L-Methionine1,2,10-Trihydroxydihydro-Trans-Linalyl Oxide 7-O-Beta-D-GlucopyranosideStyrenePe(P-16:0/3:0)2-Oxopiperidine-3-CarbohydrazideTuliposide AL-Pyridosine4-HydroxyprolineButylisopropylaminePhenylacetoneLauryldiethanolamineThiamine PyrophosphatePe(Pge1/P-18:0)LinoleamideIle-ProAcridin-9-Amine1,2,4-Benzotriazin-3-Amine 1-OxideGlyceryl Palmitate1-(6-Morpholin-4-Ylpyrimidin-4-Yl)-N-(Pyridin-2-Ylmethyl)Imidazole-4-CarboxamideSpb(16:0_2O)3-Phenyl-1H-Pyrazole-4-CarbaldehydeRobalzotanDeoxyuridineAlpha-MethyltryptamineDihydrozeatinEstranePe(19:0/0:0)Leucylproline6-NitroveratraldehydePhosphatidylcholine Lyso 18:0Cer(8:1_2O/4:0)4-Guanidinobutanoic AcidCer(14:3_2O/4:0)BenzylpenicilloylLeukotriene C4 Methyl EsterFarnesyl AcetoneIsovitexinNorleucineLpc(20:1-Sn1)MaltotetraoseAllyl Cinnamate5-L-Glutamyl-L-AlaninePhytosphingosineMethacholineGpetn(14:1/20:4)1-Nitro-5,6-Dihydroxy-DihydronaphthaleneLpe(14:1)N-Acetyl-B-GlucosaminylaminePe(O-8:0/10:0)FluocortinPonasterone AGamma-Glutamyl-Beta-CyanoalanineHomoarginineThiazolineCyclohexa-1,2,4,5-TetraenePe(Pge2/P-18:1(9Z))Coumarin6-Hydroxy-2-AminopurineCyclophosphamide8-Hydroxy-4(6)-Lactarene-5,14-DiolSpermine(Gamma-Glutamyl-Gamma-Glutamyl)-S-MethylcysteinePhe-ArgHypoxanthine13E-DocosenamideMethyl JasmonateHomoserine5-Aminolevulinic AcidQuisqualic AcidSm(8:1_2O/18:2)Methyl NicotinateMethionine Methyl EsterGln-Leu-Lys6-AzauridineN,N-DimethylanilineBeta-D-RibosylnicotinateCis-And Trans-Ethyl 2,4-Dimethyl-1,3-Dioxolane-2-AcetateCetefloxacin7-Aminoheptanoic Acid3-Methylamino-1,2-Propandiol(E)-26,27-Dinorergosta-4,22-Dien-3-OneGlu-Ile2-Methyl-4-(Pyridin-2-Yl)But-3-Yn-2-Ol3-Methoxypropylamine1-Pentanesulfenothioic AcidTimonacicGly-Phe3-(3-Indolyl)-2-Oxopropanoic AcidMethyl 2-Amino-3-(1H-Indol-3-Yl)PropanoateL-SerineZ-Lly-FmkGarcinoic AcidSpb(16:1_2O)DidanosineN-Fructosyl IsoleucineLumichrome2-Aminohexadecanoic Acid1-Hydroxy-2,2,5,5-Tetramethylpyrrolidine-3-CarboxamideChapelieric AcidGonal2-Aminobenzenesulfonic AcidLys-Gly13,14-Dihydroprostaglandin F2AlphaPe(Pgd2/P-18:1(11Z))2,5-DimethylbenzaldehydeSerylprolineIndole-3-AcetamideTyr-Ser-AlaPrehumulinic AcidNicotinamide Mononucleotide2-[(2S)-2,6-Diaminohexanoyl]Oxyacetic AcidBis(2-Ethylhexyl) SebacateLeptomycin BL-MethionineN-Oleoyl ArginineGlycyl-L-LeucineNicotinamideT-4-Aminocrotonic Acid (Taca)Proliferin11,17-Dihydroxy-3,20-Dioxopregn-4-En-21-Yl AcetateSorbitan PalmitateLycoperodine IGamma-Glutamyl-S-MethylcysteineD-DopachromeCoenzyme AMethyl L-TyrosinateN'-HydroxymethylnorcotinineAsp-IleGlu-Gln-Tyr3,4-MethylenedioxyamphetamineL-AcetylcarnitineNoradrenochromePro-ValS-AdenosylmethionineIsavuconazole4-Amino-5-Hydroxymethyl-2-Methylpyrimidine9-Octadecynoic AcidCyclohexanecarboxylic AcidAla PheThymidinePolyoxyethylene 40 MonostearatePantetheine3Beta-Hydroxy-(22E,24R)-Ergosta-5,8,22-Trien-7-OneGlu Phe(4S,8R)-8,9-Dihydroxy-P-Menth-1(6)-En-2-OneSucrose Acetate IsobutyrateN,N-Dimethylarginine11-Hydroxy-9-Tridecenoic Acid3,4-Difluoro-N-(2-Methoxyquinolin-8-Yl)Benzamide1-(Beta-D-Ribofuranosyl)-1,4-DihydronicotinamideLaurolactamErucamideN-Methyllysine20-Hydroxyecdysone2'-Deoxyadenosine 3'-MonophosphateN6-(1,2-Dicarboxyethyl)-Amp(+)-1-Methylpropyl 3-(Methylthio)-2-Propenyl Disulfide1H-Indol-3-Yl(Pyridin-2-Yl)MethanolNicotinic AcidArg Cys GluLys-Ser-LysOxypurinolDimethyl PhosphateMet-Glu-GluPaz-Pc(S,S)-Butane-2,3-DiolPro IleHexadecanamideN-(3-Methyl-[1,2,4]Triazolo[4,3-A]Pyridin-8-Yl)Pyrazine-2-Carboxamide9,10-Epoxyoctadecanoic AcidLpc(18:1)Gly-TyrN6-Carbamoyl-L-ThreonyladenosineFuscaxanthone AEpinephrineAsp-ValAvocadeneGpcho(12:0/18:4)1-[3,4-Dihydroxy-5-(Hydroxymethyl)Oxolan-2-Yl]-4-Hydroxyhydropyridin-2-OnePentaethylene GlycolB-OctylglucosideLeucogenenolCerebronic AcidAcetoleinNorvalineDaidzeinBiotinyl-5'-AmpGamithromycinfalseNon-targeted metabolomic profiling reveals Flo8-associated metabolic alterations in Candida glabrataFlo8 is a transcriptional regulator involved in multiple physiological processes in Candida glabrata. To investigate the metabolic changes associated with Flo8, non-targeted metabolomic profiling was performed using the reference strain and a FLO8 deletion mutant. Intracellular metabolites were extracted from cells cultured under the same conditions and analyzed by liquid chromatography–mass spectrometry. Comparative metabolomic analysis revealed extensive alterations in metabolic pathways related to vitamin B6 metabolism, amino acid metabolism and glutathione metabolism. In particular, the FLO8 deletion mutant exhibited altered levels of pyridoxal 5′-phosphate and its related vitamers, together with changes in glutathione-associated metabolites. This dataset provides a global view of Flo8-dependent metabolic regulation and supports further investigation of the relationship between PLP homeostasis, mitochondrial function, and iron metabolism in C. 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