MetaboLights

application/xml

ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/m_MTBLS15171_LC-MS_negative_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/s_MTBLS15171.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/a_MTBLS15171_LC-MS_negative_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_COMP_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_COMP_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_5.7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_6.8_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_COMP_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_comp_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_5.7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_6.8_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_WT_5.7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_KO_5.7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_WT_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_6.8_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_WT_5.7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_6.8_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/PfkB_comp_6.8_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_KO_5.7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/DosR_KO_6.8_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171/FILES/RAW_FILES/Rv0998_comp_5.7_4.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15171Standards of sugar phosphates were purchased from Sigma-Aldrich: α-D-fructose-6-phosphate (F1502-1G), α-D-glucose-6-phosphate (G7250), α-D-glucose-1-phosphate (G7000-5G), calbiochem α-D-mannose-6-phosphate (444100-50MG). The metabolites in the samples were identified based on their m/z values and retention times compared to those of the standards.MetaboLightsPublicLiquid Chromatography MS - negative - hilicThe data were acquired with an Agilent 1290 Infinity II UHPLC coupled to a 6545 LC/Q-TOF system. Chromatographic separation was performed with an Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 × 100 mm, 2.7 μm (p/n 675775-924)) column. The HILIC-Z methodology was optimized for polar acidic metabolites. Column compartment was set at 50°C. For easy and consistent mobile-phase preparation, a concentrated 10 × solution consisting of 100 mM ammonium acetate (pH 9.0) in water was prepared to produce mobile phases A and B. Mobile phase A consisted of 10 mM ammonium acetate in water (pH 9) with a 5 μM Agilent InfinityLab deactivator additive (p/n 5191-4506), and mobile phase B consisted of 10 mM ammonium acetate (pH 9) in 10:90 (v:v) water/acetonitrile with a 5 μM Agilent InfinityLab deactivator additive (p/n 5191-4506). The following gradient was applied at a flow rate of 0.25 ml/min: 0 min, 96% B; 2 min, 96% B; 5.5 min, 88% B; 8.5 min, 88% B; 9 min, 86% B; 14 min, 86% B; 17 min, 82% B; 23 min, 65% B; 24 min, 65% B; 24.5 min, 96% B; 26 min, 96% B and 3-min of re-equilibration at 96% B. Lysine acetyltransferase Rv0998 coordinates transcriptional and metabolic adaptation of Mycobacterium tuberculosis to acidic pH.Imperial College LondonYi LiuWhole Organismmass spectrometry assayBacteria are metabolically quenched in extraction solution (40% acetonitrile, 40% methanol, 20% ddH2O). Samples were transferred to microtubes containing 0.1 mm acid-washed zirconia beads and lysed using a FastPrep-24 homogeniser twice at 6.0 m/s for 30 seconds, with a 5-minute interval. Supernatants were transferred to 0.22 μm spin X column filters (Costar) and filtered twice by centrifuging at 15,000 rpm for 30 minutes. After filtration, the flowthrough was mixed with equal volume of acetonitrile and centrifuged at 15,000 rpm for 10 minutes. 100 µL of mixture was loaded into polypropylene snap vials (Agilent Technologies) for LC-MS analysis.Mycobacterium tuberculosishttps://www.ebi.ac.uk/metabolights/MTBLS15171Yi Liu. Imperial College London. Centre for Bacterial Resistance Biology, Imperial College London, London, United Kingdom. yi.liu13@imperial.ac.uk.Gerald Larrouy-Maumus. Imperial College London. Centre for Bacterial Resistance Biology, Imperial College London, London, United Kingdom. g.larrouy-maumus@imperial.ac.uk.MassHunter Qualitative Analysis software was used to check the extracted ion chromatograms of the m/z values of interest and obtain their retention times, which was used to construct the personal compound database and library (PCDL) for subsequent analysis. MassHunter Profinder B8.0 was used to extract the stable isotope labelling patterns from PCDL.PHTechnical replicateBiological replicateyi.liu13@imperial.ac.uk13C-labelled 7H10 agar was prepared by substituting dextrose in the supplement with [U-13C6] glucose at the same concentration. Mtb strains were inoculated onto nitrocellulose membranes and grown on 7H10 agar for 5 days to generate biomass. The membranes were transferred to 7H10 agars plates at either pH 6.8 or pH 5.7 containing [U-13C6] glucose for overnight incubation. After incubation, bacteria were harvested from the membranes.Metabolomicscell lysatetargeted metabolomic assay13CMetabolomicstargeted analysisMycobacterium tuberculosisCyclic AMPDNA-binding transcriptional activator DevR/DosR (Mycobacterium tuberculosis H37Rv)Agilent 1290 Infinity HPLCAgilent 6545 Q-TOFWhole Organismcell lysatetargeted metabolomic assay13CMetabolomicstargeted analysisMycobacterium tuberculosisCyclic AMPDNA-binding transcriptional activator DevR/DosR (Mycobacterium tuberculosis H37Rv)Agilent 1290 Infinity HPLCAgilent 6545 Q-TOFWhole OrganismAccurate MS was performed using an Agilent Accurate Mass 6545 QTOF apparatus. Dynamic mass axis calibration was achieved by continuous infusion after the chromatography of a reference mass solution using an isocratic pump connected to an electrospray ionization source operated in negative-ion mode. The following parameters were used: gas temperature, 225°C; drying gas, 13 l min-1; sheath gas temperature, 350 °C; nebulizer pressure, 35 psi; sheath gas flow, 12 l min-1; capillary voltage, 3,500 V; nozzle voltage, 0 V; fragmentor voltage, 125 V; skimmer 45V and octupole 1 RF voltage, 750V. The data were collected in centroid 4 GHz (extended dynamic range) mode.falseLysine acetyltransferase Rv0998 coordinates transcriptional and metabolic adaptation of Mycobacterium tuberculosis to acidic pHMycobacterium tuberculosis (Mtb) survives host defences by adapting to host conditions, including acid stress, during infection. A critical survival strategy involves pH homeostasis, mediated by complex signalling systems that regulate metabolic reprogramming and stress responses. While the lysine acetyltransferase Rv0998 is known to support Mtb hypoxia adaptation and metabolic flexibility, its role in acid stress remains unexplored. Here, we investigate the consequences of rv0998 deletion in 7H9 with glucose as the major carbon source. RNA-seq analysis showed that loss of Rv0998 broadly altered the transcriptional response to acidic pH, including changes in the DosR regulon and pfkB/rv2029c. Stable isotope tracing with [U-13C6]-glucose further showed reduced fractional 13C labelling across central carbon metabolites in the rv0998 mutant, with the largest decrease at acidic pH observed in upper glycolytic and connected hexose-phosphate intermediates. Growth and glucose-labelling phenotypes of dosR and pfkB mutants provided focused examples of stress-responsive and carbon-metabolic processes associated with the broader Rv0998 response. These findings indicate that Rv0998 contributes to coordinated transcriptional and metabolic adaptation of Mtb to acidic pH and provide a basis for dissecting how stress-responsive regulatory programmes are coupled to carbon metabolism during acid adaptation.2026-09-032026-07-28MTBLS15171