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supplied methods document did not report compound-identification databases or matching thresholds. Identified metabolites were mapped to KEGG pathways for annotation and enrichment analysis. Pathway p values were FDR-corrected, with FDR <= 0.05 used for significance; MSEA used the SMPDB library and Fisher's exact test through the R package MSEAp.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseReverse-phase liquid chromatography was performed using an Agilent 1290 Infinity UHPLC system fitted with an ACQUITY UPLC BEH C18 column (1.7 um, 2.1 x 100 mm). The service-provider document did not report the mobile phases, gradient, flow rate, column temperature or injection volume.Influenza A virus H1N1 infection alters sphingolipid metabolism in A549 cells.Nanjing Agricultural UniversityXIFENG HUA-549 cellmass spectrometry assayMetabolites were extracted from A549 cell samples using the metabolomics service provider's standard untargeted LC-MS extraction workflow. The supplied methods document did not report solvent composition, sample-to-solvent ratio, extraction time, temperature or centrifugation conditions.Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS15182XIFENG HU. Nanjing Agricultural University. xifenghu333@gmail.com.Jihui Ping. Nanjing Agricultural University. jihui.ping@njau.edu.cn.Positive- and negative-ion data were analyzed separately. Quality control was evaluated by principal component analysis. Hierarchical clustering used average linkage, and heatmap values were z-score normalized. PCA was performed in R with gmodels v2.18.1, PLS-DA with ropls, and OPLS-DA with seven-fold cross-validation and 200 permutation tests. Differential metabolites were defined using VIP >= 1 and a two-group t-test p value < 0.05.Treatmentxifenghu333@gmail.comA549 human lung adenocarcinoma cell samples comprised six mock-infected samples and six samples infected with influenza A virus H1N1 WSN strain. Three pooled quality-control samples were included to assess LC-MS analytical stability.MetabolomicsA-549 cellpooled quality control sampleMetabolomicsuntargeted metabolomicsviral infectionuntargeted analysiscell cultureHomo sapiensAgilent 1290 Infinity UHPLCSCIEX TripleTOF 6600experimental sampleA-549 cellpooled quality control sampleMetabolomicsuntargeted metabolomicsviral infectionuntargeted analysiscell cultureHomo sapiensAgilent 1290 Infinity UHPLCSCIEX TripleTOF 6600experimental sampleMass spectrometric detection was performed using a SCIEX TripleTOF 6600 instrument in separate positive- and negative-ion modes over an m/z range of 50-1500. The supplied methods document did not report the ion-source and mass-analyzer acquisition parameters.D-myo-inositol-1,4-diphosphateTaurineD-FructoseSorbitol 6-phosphateIle-AspAllantoin2'-deoxyadenosine 5'-monophosphateGamma-Glutamylcysteine.alpha.-L-Glu-GlySalmeterolsn-Glycerol 3-phosphoethanolamineIle-LysFormylanthranilic acidLithocholic acidAdenosine 5'-diphosphoriboseDeoxyinosine3-hydroxy-3-methylglutaric acidGamma-l-glutamyl-l-glutamic acid3-PhosphoserinePhosphoenolpyruvateAdenosine 3'-monophosphateAdenosine 5'-triphosphate (atp)L-LeucineD-SorbitolUridine 5'-triphosphateArctigeninPyridoxalDocosatrienoic acidGln-gluN-Acetyl-Asp-GluSuccinate2-oxoadipic acidGlycolate7-methylthioheptyl glucosinolateGarcinolPhenolHypericinGly-GluDL-tryptophanLeu-ThrL-glutathione, reducedMesaconic acid1,11-undecanedicarboxylic acidCholesteryl sulfatePodophyllotoxinRicinoleic acidThymidine 5'-triphosphate6'-sialyl-n-acetyllactosamineArachidonic Acid (peroxide free)L-methionineEthyl sulfateGlycinePenicillin GD-MannoseVal-AlaGanoderic acid aVal-IlePinocembrinOleic acidGlutaric acidN-acetyl-l-aspartic acidBeta-hydroxybutyrate1,2-dioleoyl-sn-glycero-3-phosphateZoledronic acidPro-gly4-HydroxybenzoateL-cystineCis,cis-muconic acidPentadecanoic acidGlutathioneFructose 1,6-diphosphate[6]-gingerolL-fucose-1-phosphateVal-PhePhenylalanineN-acetylaspartylglutamic acidPantothenateCyclohexanesulfamic acidD-Mannose 1-phosphatealpha-D-Galactose 1-phosphateBeta-AlanineN-AcetylmannosaminePyridoxal (Vitamin B6)AnetholeUracilPantothenic acidGlycerol 3-phosphateIndolelactic acidAcetolTaurocholateIsovaleric acid4-pyridoxic acidGlyceraldehydeCitrate3'-o-methylguanosineCytidine 5'-monophosphate.beta.-nicotinamide adenine dinucleotide(NAD)GinkgotoxinCytosineGlyceric acidValerenic acid6-acetylmorphineAsp-AspPhenaceturic acidPyridoxamine 5'-phosphateN-acetyl-l-glutamateHexadecanedioic acid3-hydroxyglutaric acidIle-LeuCis-4,7,10,13,16,19-docosahexaenoic acidL-Malic acidL-threonateMalateMyristic acidHis-MetTyr-ArgUridineHypoxanthine1-Deoxy-D-xylulose 5-phosphateHis-HisThymidine 5'-diphosphateAdenosine 5'-diphosphateD-ThreitolIndoleD-GlucuronateLomefloxacin1-oleoyl-sn-glycero-3-phosphocholineProlineDantroleneN-Acetylaspartylglutamate (NAAG)3-Methylphenylacetic acid.alpha.-d-galactose 1-phosphate7-hydroxymethotrexateCyclic adenosine diphosphate ribose1-(9z,12z-octadecadienoyl)-2-hydroxy-sn-glycero-3-phosphoethanolamineN-acetyltryptophanPropiolic acidTrans-vaccenic acidOphthalmic acidGdp-l-fucoseUdp-d-glucoseN-acetylglucosaminylasparagine2-methyl-3-hydroxybutyric acidPyruvateAdenineD-ribulose 5-phosphateUric acidD-(+)-ribonic acid .gamma.-lactone2-ketohexanoic acidGuanidoacetic acidProgoitrinPhe-serLinoleic acidInosineUdp-xyloseD-Erythrose 4-phosphate5-Amino-4-carbamoylimidazole (AICA)Hypotaurinegamma-L-Glutamyl-L-valine9,10-dihydroxy-12z-octadecenoic acidDL-tyrosineLumichromeGlutamineDL-threonineBenzoic acid2,5,7,8-tetramethyl-2-(.beta.-carboxyethyl)-6-hydroxychromanSucrose3-Hydroxycapric acidN-acetyl-l-methioninePhe-lysPalmitic acidCytidine monophosphate n-acetylneuraminic acidErythritolUridine 5'-triphosphate (UTP)Ala-GlyHistidineLys-GlyLeu-His6'-sialyllactose6-phospho-d-gluconateLeu-AlaD-aspartic acid(-)-riboflavinD-gluconate3-hydroxymyristic acidL-Ribulose5'-phosphoribosyl-5-amino-4-imidazolecarboxamide (aicar)LonicerinRauwolscineRibitolL-IditolDihydrouracilHomocitrateN-Acetyl-D-Glucosamine 6-PhosphateGlycerophosphocholineHesperidinGlutamic acidP-toluenesulfonic acid3-Methylglutaric acidGambogic acidS-adenosyl-l-homocysteineNADH3-Methoxy-4-Hydroxyphenylglycol SulfateUdp-n-acetylglucosamineAlpha-ketoisovaleric acidThiamine pyrophosphate (tpp)Uridine 5'-diphosphate (UDP)Butylated hydroxyanisoleHydroxyphenyllactic acidMyo-inositolAcamprosateIsomaltoseXanthineUridine 5'-monophosphate.beta.-nicotinamide adenine dinucleotide phosphate1,2-benzenedicarboxylic acidGuanosine triphosphateD-turanoseL-GlutamineD-glucosamine 6-phosphateVal-AsnValineCytidine 5'-diphosphateVal-AspAsparagineNovobiocinAdenosine 5'-monophosphateArtemisetinVal-LysMaleamic acidHydroxyisocaproic acid4-Aminobutyric acid(GABA)Glutathione disulfideN-acetylglutamineD-2-phosphoglyceric acidGlu-LysGln-hisGuanosine diphosphate mannose5-HydroxymethyluracilLabetalolInosine 5'-monophosphate (IMP)Glycocholic acidGlu-ThrOctadecanoic acidLeu-LeuGuanosine 5'-diphosphateAdenosine monophosphate (AMP)Oseltamivir acidD-erythro-imidazolylglycerol phosphateIndole-3-carboxaldehydeStearic acidUridine diphosphate glucose(UDP-D-Glucose)3-hydroxyoctanoic acidAla-ThrD-(+)-GalactoseOxyacanthineHistaminePro-AspIsocitrateAtorvastatinAcetyl coenzyme aProtoporphyrin ixPyruvaldehydeGly-AspAdenosine 5'-phosphosulfatePterineMitragynineTaurochenodeoxycholateLeucineDodecanoic acid16-hydroxyhexadecanoic acid1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-n-methylFosfomycinCis-9-palmitoleic acidCitramalic acid3,4-dihydroxyhydrocinnamic acid1-stearoyl-2-linoleoyl-sn-glycero-3-phosphoethanolamineUridine 5'-diphosphateD-mannose 6-phosphateDihydrothymineL-CarnosineUrocanate11,14,17-eicosatrienoic acid, (z,z,z)-Leu-Arg3-deoxy-d-glycero-d-galacto-2-nonulosonic acidCitramalateGlutathione, oxidizedL-pyroglutamic acidDL-serinegamma-L-Glutamyl-L-phenylalanineGlycerophosphate(2)Sedoheptulose 7-phosphateBiocytinGuanosine 5'-monophosphateDeoxycholic acidPyridoxal phosphateSarcosineVitamin cProgesteronefalseInfluenza A virus H1N1 infection alters sphingolipid metabolism in A549 cellsThis study investigates metabolic alterations in A549 cells infected with influenza A virus H1N1 (WSN strain). Untargeted metabolomics analysis was performed using liquid chromatography coupled with mass spectrometry (LC-MS) in both positive and negative ionization modes. The study aims to characterize metabolic changes associated with influenza virus infection, with a focus on sphingolipid metabolism.2026-07-292026-07-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