MetaboLights

application/xml

ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/m_MTBLS15207_GC-MS_positive_high-polarity_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/a_MTBLS15207_GC-MS_positive_high-polarity.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/s_MTBLS15207.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/FILES/RAW_FILES/Q5_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/FILES/RAW_FILES/DMSO_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/FILES/RAW_FILES/Q5_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/FILES/RAW_FILES/Q5_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/FILES/RAW_FILES/DMSO_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207/FILES/RAW_FILES/DMSO_2.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15207Feature identification and metabolite annotation were performed using Chroma TOF software (version 4.3x, LECO Corporation). The raw data processing workflow included peak extraction, baseline filtering and correction, deconvolution, peak alignment, integration, and spectrum matching. Compound identification was achieved by comparing mass spectra against the NIST mass spectral library. Retention indices (RI) were calculated using a series of n_alkane standards (C9_C23) and were used as an additional criterion to support metabolite annotation. MetaboLightsPublicGas Chromatography MS - positive - high-polarityGC-MS analysis was performed using an Agilent 8890 gas chromatograph system coupled with an Agilent 5977B mass spectrometer. The system was equipped with a DB-Wax column (30 m × 250 μm × 0.25 μm; Agilent). In the SPME cycle of the PAL rail system, the incubate temperature was 60 °C, with a preheat time of 15 min, an incubation time of 30 min, and a desorption time of 4 min. The injection was performed in splitless mode. Helium was used as the carrier gas, with a front inlet purge flow of 3 mL/min and a constant column flow rate of 1 mL/min. The oven temperature program was as follows: initial temperature 40 °C held for 4 min, raised to 245 °C at a rate of 5 °C/min, and held for 5 min. The injection temperature was 250 °C, and the transfer line temperature was 250 °C.Untargeted metabolomics analysis of volatile organic compounds emitted by Streptomyces sp. 30177 treated with C8-HSL.qin xiaoSouth China Normal Universitywhole cellmass spectrometry assayTake sample into the 20 mL headspace bottle, Add 1 mL of saturated sodium chloride solution [containing internal standard solution (2-octanol 10 mg/L stock in H2O) at a volume ratio of 100:1]; All samples were analyzed by gas chromatograph system coupled with a spectrometer(GC_MS).Streptomyces sp. 30177https://www.ebi.ac.uk/metabolights/MTBLS15207Dan Wang. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. No. 66, Jinying Road, Tianhe District, Guangzhou, Guangdong Province, China. wangdanzh@gdaas.cn.Wenjie Gu. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. No. 66, Jinying Road, Tianhe District, Guangzhou, Guangdong Province, China. guwenjie0818@163.com.Chroma TOF 4.3X software of LECO Corporation and Nist database were used for raw peaks exacting, the data baselines filtering and calibration of the baseline, peak alignment, deconvolution analysis, peak identification, integration and spectrum match of the peak areaTreatment19854815383@163.comStreptomyces sp. 30177 was co_cultured with C8_HSL, with Streptomyces sp. 30177 cultured alone serving as the control. The cell pellets were collected for untargeted metabolomics analysis of volatile organic compounds (VOCs).MetabolomicsMetabolomicswhole cell8890Buntargeted analysisAgilent 5977B MSDC8-HSLStreptomycesVolatile Organic Compoundsexperimental blankStreptomyces sp. 30177Metabolomicswhole cell8890Buntargeted analysisAgilent 5977B MSDC8-HSLStreptomycesVolatile Organic Compoundsexperimental blankStreptomyces sp. 30177The mass spectrometer was an Agilent 5977B equipped with an electron impact (EI) ion source operating in positive ion mode (EI+). The ionization energy was -70 eV. The ion source and quadrupole temperatures were set to 230 °C and 150 °C, respectively. Mass spectra were acquired in full scan mode over the m/z range of 20–400. A solvent delay of 2.37 min was applied.falseUntargeted metabolomics analysis of volatile organic compounds emitted by Streptomyces sp. 30177 treated with C8-HSLThis study aimed to analyze the volatile organic compounds (VOCs) produced by Streptomyces sp. 30177 in response to C8-HSL treatment.2026-07-302026-07-30MTBLS15207