Features were retained if ≥80% nonzero values in at least one group. Compounds were identified by matching accuracy m/z mass (m/z error <10 ppm), and MS/MS spectra with an in-house database established with available authentic standards.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - hilic"],"chromatography_protocol":["For hydrophilic interaction liquid chromatography (HILIC), separation was achieved on an ACQUITY UPLC BEH Amide column (2.1 mm × 100 mm, 1.7 µm; Waters, Ireland) maintained at 25°C. The flow rate was 0.5 mL/min with a 2 μL injection volume. Mobile phases A: water with 25 mM ammonium acetate and 25 mM ammonium hydroxide, and B: acetonitrile. The gradient program was as follows: 0-0.5 min, 95% B; 0.5-7 min, linear decrease to 65% B; 7-8 min, linear decrease to 40% B; 8-9 min, 40% B; 9-9.1 min, linear increase to 95% B; 9.1-12 min, 95% B.
"],"publication":["Gut Microbiome Dysbiosis Is Associated with Aldosterone Overproduction in Idiopathic Hyperaldosteronism."],"submitter_affiliation":["chongqing medical university"],"submitter_name":["yuanliang jiang"],"organism_part":["blood plasma"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Plasma samples were thawed at 4°C and 100 μL aliquots were mixed with 400 μL cold (-20°C) protein precipitation solvent (LC-MS grade methanol [Fisher Chemical, USA] and acetonitrile [Merck, USA], 1:1, v/v). After centrifugation at 14000 g for 20 min at 4°C, the supernatant was collected and dried under vacuum. The residue was reconstituted in 100 μL acetonitrile/water (1:1, v/v) and centrifuged at 14000 g for 15 min at 4 °C. Then supernatants were analyzed in randomized sequence with pooled Quality Control (QC) samples injected every 7 samples to monitor system stability and data reliability.
"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15210"],"author":["Yifan He.","Yunjie Xiong.","Ying Jing.","Qifu Li.","Shuangshuang Zhu.","Yuanliang Jiang. chongqing medical university. jyl9611@163.com.","Shumin Yang. The First Affiliated Hospital of Chongqing Medical University. 443068494@qq.com.","Jinbo Hu.","Qinglian Zeng.","Linqiang Ma.","Shuangxin Qi.","Qi Zhang."],"data_transformation_protocol":["Raw data were converted to mzXML format using ProteoWizard, followed by peak alignment, retention time correction, and peak area extraction with XCMS. Peak picking employed centWave m/z = 10 ppm, peakwidth = 10-60 s, prefilter = c (10, 100). Peak grouping used bwi = 5, m/zwid = 0.025, minfrac = 0.5. CAMERA annotated isotopes and adducts.
"],"study_factor":["Disease status"],"submitter_email":["jyl9611@163.com"],"sample_collection_protocol":["Plasma samples were collected from fasting participants in the morning prior to treatment. Blood was drawn into EDTA tubes, centrifuged to separate plasma, aliquoted, and stored at −80 °C until untargeted LC–MS analysis. Samples were obtained from 29 patients with idiopathic hyperaldosteronism and 30 healthy controls enrolled at the First Affiliated Hospital of Chongqing Medical University.
"],"omics_type":["Metabolomics"],"study_design":["pooled quality control sample","Metabolomics","Vanquish","blood plasma","untargeted analysis","case-control study","Homo sapiens","Idiopathic Hyperaldosteronism","Orbitrap Exploris 480","metabolite profiling","experimental sample"],"curator_keywords":["pooled quality control sample","Metabolomics","Vanquish","blood plasma","untargeted analysis","case-control study","Homo sapiens","Idiopathic Hyperaldosteronism","Orbitrap Exploris 480","metabolite profiling","experimental sample"],"mass_spectrometry_protocol":["Electrospray ionization (ESI) settings were: ion source gas1 (Gas1) as 50, ion source gas2 (Gas2) as 2; ion transfer tube temperature, 350°C; spray voltage: +3500 V/-2800V. Full-scan MS parameters: mass range m/z 70-1200 Da, resolving power 60000, maximum injection time 100ms. Data-dependent MS/MS acquisition used the same m/z range and resolution, with a dynamic exclusion time within 4 s.
"],"additional_accession":[]},"is_claimable":false,"name":"Plasma metabolomic profiling reveals altered metabolic pathways associated with idiopathic hyperaldosteronism","description":"This study investigated plasma metabolic alterations associated with idiopathic hyperaldosteronism using untargeted liquid chromatography coupled with high-resolution mass spectrometry. Plasma samples from patients with idiopathic hyperaldosteronism and healthy controls were analyzed using a Vanquis UHPLC system coupled to an Orbitrap Exploris 480 mass spectrometer.","dates":{"publication":"2026-08-07","submission":"2026-07-30"},"accession":"MTBLS15210","cross_references":{}}