MetaboLights

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were retained if ≥80% nonzero values in at least one group. Compounds were identified by matching accuracy m/z mass (m/z error <10 ppm), and MS/MS spectra with an in-house database established with available authentic standards.MetaboLightsPublicLiquid Chromatography MS - alternating - hilicFor hydrophilic interaction liquid chromatography (HILIC), separation was achieved on an ACQUITY UPLC BEH Amide column (2.1 mm × 100 mm, 1.7 µm; Waters, Ireland) maintained at 25°C. The flow rate was 0.5 mL/min with a 2 μL injection volume. Mobile phases A: water with 25 mM ammonium acetate and 25 mM ammonium hydroxide, and B: acetonitrile. The gradient program was as follows: 0-0.5 min, 95% B; 0.5-7 min, linear decrease to 65% B; 7-8 min, linear decrease to 40% B; 8-9 min, 40% B; 9-9.1 min, linear increase to 95% B; 9.1-12 min, 95% B. Gut Microbiome Dysbiosis Is Associated with Aldosterone Overproduction in Idiopathic Hyperaldosteronism.chongqing medical universityyuanliang jiangblood plasmamass spectrometry assayPlasma samples were thawed at 4°C and 100 μL aliquots were mixed with 400 μL cold (-20°C) protein precipitation solvent (LC-MS grade methanol [Fisher Chemical, USA] and acetonitrile [Merck, USA], 1:1, v/v). After centrifugation at 14000 g for 20 min at 4°C, the supernatant was collected and dried under vacuum. The residue was reconstituted in 100 μL acetonitrile/water (1:1, v/v) and centrifuged at 14000 g for 15 min at 4 °C. Then supernatants were analyzed in randomized sequence with pooled Quality Control (QC) samples injected every 7 samples to monitor system stability and data reliability.Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS15210Yifan He.Yunjie Xiong.Ying Jing.Qifu Li.Shuangshuang Zhu.Yuanliang Jiang. chongqing medical university. jyl9611@163.com.Shumin Yang. The First Affiliated Hospital of Chongqing Medical University. 443068494@qq.com.Jinbo Hu.Qinglian Zeng.Linqiang Ma.Shuangxin Qi.Qi Zhang.Raw data were converted to mzXML format using ProteoWizard, followed by peak alignment, retention time correction, and peak area extraction with XCMS. Peak picking employed centWave m/z = 10 ppm, peakwidth = 10-60 s, prefilter = c (10, 100). Peak grouping used bwi = 5, m/zwid = 0.025, minfrac = 0.5. CAMERA annotated isotopes and adducts.Disease statusjyl9611@163.comPlasma samples were collected from fasting participants in the morning prior to treatment. Blood was drawn into EDTA tubes, centrifuged to separate plasma, aliquoted, and stored at −80 °C until untargeted LC–MS analysis. Samples were obtained from 29 patients with idiopathic hyperaldosteronism and 30 healthy controls enrolled at the First Affiliated Hospital of Chongqing Medical University.Metabolomicspooled quality control sampleMetabolomicsVanquishblood plasmauntargeted analysiscase-control studyHomo sapiensIdiopathic HyperaldosteronismOrbitrap Exploris 480metabolite profilingexperimental samplepooled quality control sampleMetabolomicsVanquishblood plasmauntargeted analysiscase-control studyHomo sapiensIdiopathic HyperaldosteronismOrbitrap Exploris 480metabolite profilingexperimental sampleElectrospray ionization (ESI) settings were: ion source gas1 (Gas1) as 50, ion source gas2 (Gas2) as 2; ion transfer tube temperature, 350°C; spray voltage: +3500 V/-2800V. Full-scan MS parameters: mass range m/z 70-1200 Da, resolving power 60000, maximum injection time 100ms. Data-dependent MS/MS acquisition used the same m/z range and resolution, with a dynamic exclusion time within 4 s.falsePlasma metabolomic profiling reveals altered metabolic pathways associated with idiopathic hyperaldosteronismThis study investigated plasma metabolic alterations associated with idiopathic hyperaldosteronism using untargeted liquid chromatography coupled with high-resolution mass spectrometry. Plasma samples from patients with idiopathic hyperaldosteronism and healthy controls were analyzed using a Vanquis UHPLC system coupled to an Orbitrap Exploris 480 mass spectrometer.2026-08-072026-07-30MTBLS15210