MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/m_MTBLS15244_LC-MS_alternating_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/s_MTBLS15244.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/a_MTBLS15244_LC-MS_alternating_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/FILES/RAW_FILES/MA2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/FILES/MA2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/FILES/RAW_FILES/MA1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244/FILES/MA1.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15244<p> Raw data were processed using MS-DIAL software for peak alignment, retention time correction, and peak area extraction. Metabolite annotation was performed using accurate mass matching (mass tolerance &lt;10 ppm) and MS/MS spectral matching (mass tolerance &lt;0.01 Da) against in-house BP-Plant database (BioProfile, Shanghai).Please update this protocol description</p>MetaboLightsPublicLiquid Chromatography MS - alternating - reverse-phase<p> During the entire analysis, samples were maintained at 4 °C in the autosampler. Chromatographic separation was performed on a SHIMADZU-LC30 UHPLC system using an ACQUITY UPLC® HSS T3 column (2.1 × 100 mm, 1.8 μm; Waters, Milford, MA, USA). The injection volume was 16 μL, column temperature was set at 40 °C, and the flow rate was 0.3 mL/min.</p><p> Mobile phase A consisted of 0.1% formic acid in water, and mobile phase B consisted of 0.1% formic acid in acetonitrile. The gradient elution program was as follows:</p><p>· 0–2 min, 0% B;</p><p>· 2–6 min, linear increase from 0% to 48% B;</p><p>· 6–10 min, linear increase from 48% to 100% B;</p><p>· 10–12 min, hold at 100% B;</p><p>· 12–12.1 min, linear decrease from 100% to 0% B;</p><p>· 12.1–15 min, hold at 0% B for re-equilibration.Please update this protocol description</p>Untargeted Metabolomics of Mouse Platelet Pellets by LC-MS/MS.kang jieBAIPUblood plasmamass spectrometry assay<p> Samples were thawed at 4 °C, and 50 mg of each sample was weighed into a grinding tube containing two steel beads. Then, 400 μL of pre-chilled methanol:water solution (4:1, v/v) was added. The samples were homogenized under low temperature using a tissue lyser (−10 °C, 5 min), followed by incubation at −20 °C for 30 min. After centrifugation at 16,000 g for 20 min at 4 °C, the supernatant was collected for LC-MS/MS analysis.Please update this protocol description</p>Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS15244kang jie. shanghaibaipu. kangjie@bioprofile.cn.<p> Raw data were processed using MS-DIAL software for peak alignment, retention time correction, and peak area extraction. Metabolite annotation was performed using accurate mass matching (mass tolerance &lt;10 ppm) and MS/MS spectral matching (mass tolerance &lt;0.01 Da) against in-house BP-Plant database (BioProfile, Shanghai).</p><p> Features with more than 50% missing values within a group were removed prior to statistical analysis. The positive and negative ion data were separately normalized by total peak area before integration. Multivariate pattern recognition was conducted using Python, and data were preprocessed with unit variance scaling (UV) before statistical analysis.Please update this protocol description</p>Treatmentkangjie@bioprofile.cn<p> Samples were thawed at 4 °C, and 50 mg of each sample was weighed into a grinding tube containing two steel beads. Then, 400 μL of pre-chilled methanol:water solution (4:1, v/v) was added. The samples were homogenized under low temperature using a tissue lyser (−10 °C, 5 min), followed by incubation at −20 °C for 30 min. After centrifugation at 16,000 g for 20 min at 4 °C, the supernatant was collected for LC-MS/MS analysis.Please update this protocol description</p>MetabolomicsMetabolomicsblood plasmauntargeted analysisThermo Scientific LTQ Orbitrap ClassicShimadzu LC-30A Nexera UHPLC systembreast cancerHomo sapiensmzmineexperimental sampleuntargeted metabolite profilingMetabolomicsblood plasmaThermo Scientific LTQ Orbitrap Classicuntargeted analysisShimadzu LC-30A Nexera UHPLC systemHomo sapiensbreast cancermzmineexperimental sampleuntargeted metabolite profiling<p>Samples were analyzed in both positive and negative ion modes using electrospray ionization (ESI) on a QE Plus mass spectrometer (Thermo Scientific) equipped with a heated electrospray ionization (HESI) source. Ionization conditions were set as follows: spray voltage of 3.8 kV (+) and 3.2 kV (−), capillary temperature of 320 °C, sheath gas at 30 units, auxiliary gas at 5 units, probe heater temperature at 350 °C, and S-Lens RF level at 50.</p><p> Mass spectrometric data were acquired over a 15-minute run time with a full scan range of 75–1050 m/z at a resolution of 70,000 (FWHM at m/z 200). Data-dependent MS/MS acquisition was performed by triggering fragmentation of the top 10 most intense precursor ions following each full scan. MS2 spectra were collected at a resolution of 17,500 with higher-energy collisional dissociation (HCD) using stepped normalized collision energies of 20, 30, and 40.lease update this protocol description</p>(20R)-5alpha-cholestane-3alpha,21-diol 3,21-disulfate dityrammonium salt(1xi,3xi)-1,2,3,4-Tetrahydro-1-methyl-beta-carboline-3-carboxylic acid(-)-Arbusculin D(2-Ethoxyethoxy)acetic acid(2alpha, 3beta, 5alpha, 6beta, 11alpha)-Cholest-7-ene-2, 3, 5, 6, 9, 11, 19-heptol(25R)-3beta-hydroxycholest-5-en-7-one-26-oate(25R)-26-Hydroxycholest-4-en-3-one(2E,4E,6E)-7-Hydroxy-4-methylhepta-2,4,6-trienal(2'E,4'Z,8E)-Colneleic acid(2alpha, 3beta, 5alpha, 6beta, 9alpha, 11alpha)-Cholest-7-ene-2, 3, 5, 6, 9, 11-hexolfalseUntargeted Metabolomics of Mouse Platelet Pellets by LC-MS/MSIn this study, platelet pellets were isolated from mouse peripheral blood through gradient centrifugation to obtain high-purity platelet samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based untargeted metabolomics was performed to comprehensively profile the global metabolic changes and endogenous small-molecule metabolites in mouse platelets. All samples were subjected to strict pre-treatment, including metabolite extraction, protein removal, and chromatographic separation, to eliminate experimental interference and ensure the accuracy of mass spectrum detection. Subsequent bioinformatics analyses, including metabolite identification, differential metabolite screening, metabolic pathway enrichment, and principal component analysis, were conducted to screen distinct metabolic signatures and key enriched signaling pathways between different groups. This study aims to elucidate the metabolic characteristics of mouse platelets under specific conditions, identify potential functional metabolite biomarkers, and provide a reliable metabolic theoretical basis for exploring the physiological regulatory mechanisms and pathological roles of platelets in related diseases.2026-08-042026-08-04MTBLS15244HMDB0030087HMDB0037942HMDB0030995HMDB0062613HMDB0029581HMDB0241541HMDB0241542