Metabolite identification was achieved by comparison against version 17 of the ExpBioInsilico MS Dial public libraries (https://systemsomicslab.github.io/compms/msdial/main.html), containing negative and positive spectra respectively. This would be equivalent to levels
2 and 3 annotation confidence for analytes with and without recorded MS/MS spectra respectively, following the schematic of Schymanski et al. (2014).
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Metabolite analysis was performed using liquid chromatography–mass spectrometry (LC–MS) by connecting an Agilent 1290 Infinity II (Agilent Technologies, Waldbronn, Germany) to an electrospray ionization (ESI) source with a TripleTOF 6,600 high resolution mass spectrometer (Sciex, Darmstadt, Germany). For chromatographic separation, 10 μL were injected into an Agilent Zorbax Eclipse XDB C18 column (2.1mm × 100 mm, particle size 1.8μm) heated to 40°C. The mobile phase A consisted of 0.1% FA aqueous solution and B consisted of ACN (also 0.1% FA), starting with 90% A held for 1 min, increased to 99% B within 7 min and held for additional 3.9 min. This was followed by a decrease back to 90% A within 0.1 min and held for 3 min. The full analysis time was 15 min. The flow
rate was set at 0.5 mL/min.
"],"publication":["Carbon starvation enhances microbiologically influenced corrosion in marine offshore infrastructures. 10.3389/fmicb.2026.1908395."],"submitter_affiliation":["BAM"],"submitter_name":["Jan Lisec"],"organism_part":["culture fluid","solvent"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Metabolomic profiling was conducted on samples collected from the circulating water of the experimental systems to investigate metabolic changes associated with microbial activity and corrosion processes. At the end of the experiment, water samples were collected (from all replicates) and immediately mixed with methanol at a ratio of 0.5mL sample to 1mL methanol (Kotu et al., 2024). After thorough mixing, the extracts were kept at −20°C until subsequent analysis.
"],"organism":["sea water","blank"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15254"],"author":["Jan Lisec. Federal Institute For Materials Research and Testing. jan.lisec@bam.de.","Sara Taghavi Kalajahi. Federal Institute For Materials Research and Testing. Sara.Taghavi-Kalajahi@bam.de."],"data_transformation_protocol":["ProteiWizard msconvert was used to convert raw data to mzML. mzML files were used to generate QC-plots. MSDial processing was done on wiff files directly.
"],"study_factor":["Nutrient","Cell","Flow"],"submitter_email":["jan.lisec@bam.de"],"sample_collection_protocol":["Metabolomic profiling was conducted on samples collected from the circulating water of the experimental systems to investigate metabolic changes associated with microbial activity and corrosion processes at the end of the experiment.
"],"omics_type":["Metabolomics"],"study_design":["ultra-performance liquid chromatography-mass spectrometry","sea water","Metabolomics","ProteoWizard msconvert","MSDial","blank","liquid mobile phase","untargeted analysis","microbial induced corrosion","untargeted metabolite profiling","experimental sample","culture fluid","Agilent 1290 Infinity II UHPLC","solvent","AB SCIEX TripleTOF 6600","Seawater"],"curator_keywords":["ultra-performance liquid chromatography-mass spectrometry","sea water","Metabolomics","ProteoWizard msconvert","MSDial","blank","liquid mobile phase","untargeted analysis","microbial induced corrosion","experimental sample","untargeted metabolite profiling","culture fluid","Agilent 1290 Infinity II UHPLC","solvent","AB SCIEX TripleTOF 6600","Seawater"],"mass_spectrometry_protocol":["A TripleTOF 6,600 high resolution mass spectrometer (Sciex, Darmstadt, Germany) was used. Instrument calibration was performed after every 5th sample to ensure mass accuracy < 2 ppm. The recorded mass range was 80–1,200 Da in positive and negative
ion mode, using a source temperature of 300°C and recording MS/MS scans in information-dependent acquisition (IDA) mode.
"],"additional_accession":[]},"is_claimable":false,"name":"Carbon starvation enhances microbiologically influenced corrosion in marine offshore infrastructures","description":"an exploratory mechanistic Q17 A study to investigate how carbon availability influences microbial community structure and corrosion behavior in multispecies biofilms relevant to offshore infrastructure. Sediment from the North Sea was used as inoculum, and steel coupons were exposed under blank (no added carbon), lactate, and yeast extract conditions.","dates":{"publication":"2026-09-16","submission":"2026-08-05"},"accession":"MTBLS15254","cross_references":{}}