The metabolites were identified by searching databases, and the main databases were the HMDB (http://www.hmdb.ca/) and the self-compiled Majorbio Database (MJDB) of Majorbio Biotechnology Co., Ltd. (Shanghai, China), with MS mass error set to less than 10 ppm, to obtain metabolite information. Upload the searched data matrix to the Meiji Cloud platform (cloud. Majorbio. com) for analysis.Please update this protocol description
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["The liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an UHPLC-Q Exactive HF-X System equipped with an ACQUITY UPLC BEH C18 (100 mm × 2.1 mm i.d., 1.7 µm; Waters, Milford, USA) at Majorbio (Shanghai, China). The mobile phases consisted of 0.1% formic acid in water: acetonitrile (2:98, v/v) (solvent A) and 0.1% formic acid in acetonitrile (solvent B). 2µL injection volume eluted through the column at a flow rate of 0.40 mL/min at 40℃.Please update this protocol description
"],"publication":["Mulberry Cultivation for Saline-Alkali Land Amelioration: Metagenomic and Metabolomic Perspectives on Soil Biogeochemistry and Leaf Metabolic Profiling."],"submitter_affiliation":["Shandong Institute of Sericultureï¼Shandong Academy of Agricultural Sciences"],"submitter_name":["Yinyu Gu"],"organism_part":["leaf"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["A 100 mg solid tissue was transferred into a 2 mL centrifuge tube along with a 6 mm grinding bead. Metabolite extraction was performed with 400µL of extraction solution (methanol: water = 4:1 (v: v) containing four internal standards ( 0.02 mg/mL L-2-chlorophenylalanine, etc.). The mixtures were ground by the Wonbio-96c ( Shanghai Wanbo Biotechnology Co., LTD) frozen tissue grinder for 6 min (-10°C, 50 Hz), followed by low-temperature ultrasonic extraction for 30 min (5 °C, 40 kHz).
After incubation at −20 °C for 30 min, the samples were centrifuged for 15 min (4 °C, 13000 g), and the supernatant was used to the injection vial for LC-MS/MS analysis. 20 µL of supernatant from each sample was mixed together as quality control (QC). One QC sample was injected every 5-10 samples throughout instrumental analysis to monitor the stability of the analysis.Please update this protocol description
"],"organism":["Morus alba"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15262"],"author":["Yinyu Gu. Shandong Institute of Sericultureï¼Shandong Academy of Agricultural Sciences. guyy70@163.com."],"data_transformation_protocol":["PThe raw data was performed by Progenesis QI v3.0 (Waters Corporation, Milford, USA) software for baseline filtering, peak identification, integration, retention time correction, and peak alignment to obtain a data matrix with retention time, mass to charge ratio, and peak intensity.lease update this protocol description
"],"study_factor":["Group"],"submitter_email":["guyy70@163.com"],"sample_collection_protocol":["The samples were collected on June 5, 2026 from the 3-year-old hybrid mulberry variety Luza1 at the experimental field of Shandong Sericulture Research Institute (37.14°N, 121.14°E) in Haiyang, Shandong, and Qingfu Agriculture Co., Ltd. (37.04°N, 119.35°E) in Changyi, Shandong, China. Six healthy-looking, medium-growth plants around and in the middle of the mulberry field were randomly selected, leaf tissues from identical mulberry plants were gathered for metabolomic sequencing; the first fully unfolded mulberry leaf from each plant was selected; three leaves with intact shapes, uniform sizes, and no deformities were picked and combined into one composite leaf sample. The collected leaf samples were immediately placed in liquid nitrogen for subsequent sequencing. Please update this protocol description
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","Multi-omics study","untargeted analysis","Thermo Scientific Vanquish Flex UHPLC System","Thermo Scientific Q Exactive HF-X","Morus alba","leaf","mulberry leaf metabolites analysis","experimental sample","untargeted metabolite profiling"],"curator_keywords":["Metabolomics","Multi-omics study","untargeted analysis","Thermo Scientific Vanquish Flex UHPLC System","Thermo Scientific Q Exactive HF-X","Morus alba","leaf","mulberry leaf metabolites analysis","experimental sample","untargeted metabolite profiling"],"mass_spectrometry_protocol":["Mass spectrometer tuning parameters were as follows: ion source temperature = 425°C; sheath gas flow = 50 arb; auxiliary gas flow = 13 arb; ion-spray voltage floating (ISVF) = −3500 V (negative ionization mode) and 3000 V (positive ionization mode). Rolling normalized collision energies of 20, 40, and 60 V were applied for MS/MS fragmentation. Data-dependent acquisition (DDA) mode was adopted for data collection, with mass spectra recorded across an m/z range of 70-1050.Please update this protocol description
"],"additional_accession":[]},"is_claimable":false,"name":"Mulberry Cultivation for Saline-Alkali Land Amelioration: Metagenomic and Metabolomic Perspectives on Soil Biogeochemistry and Leaf Metabolic Profiling","description":"We conducted field trials combined with metabolomic sequencing to evaluate the saline-alkali soil remediation efficiency of mulberry trees, as well as the metabolic responses of mulberry leaves under saline-alkali stress.","dates":{"publication":"2026-08-06","submission":"2026-08-06"},"accession":"MTBLS15262","cross_references":{}}