MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/m_MTBLS15272_LC-MS_positive_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/m_MTBLS15272_LC-MS_negative_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/a_MTBLS15272_LC-MS_positive_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/s_MTBLS15272.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/a_MTBLS15272_LC-MS_negative_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_UA159_4.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_UA159_1.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_QC03.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_QC02.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_rcrR_4.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_UA159_1.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_rcrR_1.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_UA159_4.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_rcrR_2.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_rcrR_4.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_UA159_6.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_rcrR_2.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_rcrR_6.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_UA159_3.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_UA159_2.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_rcrR_5.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_rcrR_3.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_QC01.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_UA159_5.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_QC03.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_QC02.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_UA159_5.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_rcrR_5.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_QC01.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_UA159_2.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_rcrR_6.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_UA159_6.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/POS/POS_Hf28JLY_rcrR_3.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_rcrR_1.wiff.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272/FILES/RAW_FILES/NEG/NEG_Hf28JLY_UA159_3.wiff.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15272At the same time, the metabolites were identified by searching database, and the main databases were the HMDB (http://www.hmdb.ca/), Metlin ( https://metlin.scripps.edu/)  and Majorbio Database . MetaboLightsPublicLiquid Chromatography MS - positive - hilicLiquid Chromatography MS - negative - hilicThe LC-MS/MS analysis of sample was conducted on a Thermo Scientific Q Exactive HF-X system equipped with an ACQUITY HSS T3 column (100 mm × 2.1 mm i.d., 1.8 μm; Waters, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). The mobile phases consisted of 0.1% formic acid in water:acetonitrile (95:5, v/v) (solvent A) and 0.1% formic acid in acetonitrile:isopropanol:water (47.5:47.5, v/v) (solvent B). The flow rate was 0.40 mL/min and the column temperature was 40℃. The injection volume was 3 μL.RcrR: A Multifunctional Transcriptional Regulator Orchestrating the cariogenic virulence of Streptococcus mutans.he xuanKunming Medical UniversityBiofilmmass spectrometry assay 50 mg solid sample was added to a 2 mL centrifuge tube and a 6 mm diameter grinding bead was added. 400 μL of extraction solution (methanol: water = 4:1 (v:v)) containing 0.02 mg/mL of internal standard (L-2-chlorophenylalanine) was used for metabolite extraction. Samples were ground by the Wonbio-96c ( Shanghai wanbo biotechnology co., LTD) frozen tissue grinder for 6 min (-10°C, 50 Hz), followed by low-temperature ultrasonic extraction for 30 min (5°C, 40 kHz). The samples were left at -20°C for 30 min, centrifuged for 15 min (4°C, 13000 g), and the supernatant was transferred to the injection vial for LC-MS/MS analysis.Streptococcus mutanshttps://www.ebi.ac.uk/metabolights/MTBLS15272Huo Lijun. Kunming Medical University. huolijun1039@kmmu.edu.cn.he xuan. Kunming Medical University. hx707400@163.com.The pretreatment of LC/MS raw data was performed by Progenesis QI (Waters Corporation, Milford, USA) software, and a three-dimensional data matrix in CSV format was exported. The information in this three-dimensional matrix included: sample information, metabolite name and mass spectral response intensity. Internal standard peaks, as well as any known false positive peaks (including noise, column bleed, and derivatized reagent peaks), were removed from the data matrix, deredundant and peak pooled.Treatmenthx707400@163.comStreptococcus mutans isolates were preserved at -80°C prior to being transported to a service provider for untargeted metabolomic profiling.MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicsuntargeted analysisAcid toleranceThermo Scientific Q Exactive HF-XRcrRBiofilmexperimental blankStreptococcus mutansuntargeted metabolite profilingThermo Scientific Vanquish UHPLC SystemMetabolomicsuntargeted analysisAcid toleranceThermo Scientific Q Exactive HF-XRcrRBiofilmexperimental blankStreptococcus mutansuntargeted metabolite profiling The mass spectrometric data were collected using a Thermo Scientific Q Exactive HF-X equipped with an electrospray ionization (ESI) source operating in positive mode and negative mode. The optimal conditions were set as followed: source temperature at 425℃; sheath gas flow rate at 50 arb; Aux gas flow rate at 13 arb; ion-spray voltage floating (ISVF) at -3500V in negative mode and 3500V in positive mode, respectively; Normalized collision energy , 20-40-60V rolling for MS/MS. Full MS resolution was 60000, and MS/MS resolution was 7500. Data acquisition was performed with the Data Dependent Acquisition (DDA) mode. The detection was carried out over a mass range of 50-1200 m/z.Benzoyl-Dl-Arginine-NaphthylamidePs(15:0/14:0)Pa(20:5(5Z,8Z,11Z,14Z,16E)-Oh(18R)/18:3(6Z,9Z,12Z))Trp Ala Arg3-Hexanoyl-Nbd Cholesterol9,10-Epoxystearic AcidFlumethasoneIndicanCaprolactam(10E,12Z)-(9S)-9-Hydroperoxyoctadeca-10,12-Dienoic AcidGuanosine 5'-Diphosphate (Gdp)AdluponeDibutyl SebacateMethyl 3,5-Di-Tert-Butyl-4-HydroxybenzoateMetaphosphoric AcidPro-LeuDg(A-17:0/Lte4/0:0)1-(Beta-D-Glucopyranosyloxy)-3-OctanonePa(Txb2/I-14:0)2-Anthraquinonesulfonic Acid2-(Ethoxymethyl)PhenolLys Glu GlnDeoxycholic AcidLinolenic AcidD-Fructose-1,6-DiphosphateD-GlucosaminideGardneramineChlorobenzene5(S),6(R)-Dihete7-[(1R,2R,3R,5S)-3,5-Dihydroxy-2-[(3S)-3-Hydroxyoctyl]Cyclopentyl]HeptanoylcarnitineFlavin Adenine Dinucleotide (Fad)5-Hydroxycapsanthin-5,6-EpoxideEthyl MyristateManoyl OxidePs(15:0/18:4(6Z,9Z,12Z,15Z))Anisperimus2-Oxa-4,7,12-Triazatridecan-13-Oic Acid, 9-Hydroxy-5-(1-Methylethyl)-3,6-Dioxo-8,11-Bis(Phenylmethyl)-1-(2-Pyridinyl)-, 3-Pyridinylmethyl Ester, (5S,8S,9S,11S)-2-Hydroxy-4-Methoxy-3,5-Bis(3-Methylbut-2-Enyl)-6-Pentylbenzoic AcidCer(D18:1/Pge2)3-Phenyl-1-Propanol3-GonalAsiatic AcidEthyl 2-Methyl-3-Pentenoate5-(Hydroxyphenyl)-Gamma-Valerolactone-O-SulphateStyreneNicotinamide Adenine Dinucleotide PhosphateDelta-ValerobetaineVal Glu SerLauryl DiethanolamideGeranylgeraniolOphiopogonoside AFraxidinSoyasapogenol EPhthalic AcidLauryldiethanolamineLysope(0:0/24:1(15Z))Diguanosine TriphosphateUdp-N-AcetylmuraminateLinoleamidePc(16:1(9Z)/24:1(15Z))L-NorleucinePhosphateHexamethoxymethyl MelamineLysopc(22:2(13Z,16Z)/0:0)1-Acetyl-Beta-CarbolineCyclopropanecarboxamide, 1,2,2,3,3-Pentamethyl-3-Benzoyloxy-11-Oxo-12-Ursen-28-Oic Acid2,4-Hexadienyl PropionateDecapropylene Glycol2,3-EpoxyphylloquinoneNiacinamideDg(18:0/18:2(9Z,12Z)/0:0)LuseogliflozinIle Arg LeuCytidine 3'-MonophosphateNicotinamide Adenine DinucleotideEstraneBeta-D-Glucosyloxydestruxin BMg(0:0/18:3(6Z,9Z,12Z)/0:0)Ps(18:0/18:3(6Z,9Z,12Z))Bisindolylmaleimide IOleamide4-O-Methyl-A-D-Glucosyl-(1->2)-B-D-Xylosyl-(1->4)-D-XyloseCer(D18:0/18:0)Hexylamine(5Z,7Z,10Z,13Z,16Z,19Z)-4-Hydroxydocosa-5,7,10,13,16,19-HexaenoylcarnitineBis(2-Ethylhexyl) AdipatePe(14:1(9Z)/14:1(9Z))1-Cyclohexyl-3-[[1-(Hydroxymethyl)Cyclopropyl]Methyl]Urea8-Amino-7-Oxononanoic AcidTris(1-Chloro-2-Propyl) PhosphateD-Aspartic AcidHexadecanedioic AcidMjdbissp00000001Mjdbissp00000002Neuromedin B (1-3)Mjdbissp000000091-(2-Furanyl)-1-ButanoneGalactosylceramide (D18:1/18:1(9Z))Dehydrotumulosic Acid7-(3,4-Diethoxyphenyl)-3-[(4-Fluorophenyl)Methyl]Thieno[3,2-D]Pyrimidin-4-One(3S,3'S,5R,5'R,6R)-3,6-Epoxy-5,6-Dihydro-3',5,8'-Trihydroxy-Beta,Kappa-Caroten-6'-OneAla Glu GlyLaurocapramOleaside AChaps25-Hydroxyvitamin D2-25-GlucuronideMethyl 3-[[2-[4-(Dimethylamino)Phenyl]Imidazo[1,2-A]Pyrazin-3-Yl]Amino]Benzoate1-PiperideineFalecalcitriolPyridine2-Aminooctadecane-1,3-DiolHt-2 Toxin(8R,9R,10S,13R,14S)-1,2,3,4,5,6,7,8,9,10,11,12,14,15,16,17-Hexadecahydrocyclopenta[A]Phenanthrene-13-Carbaldehyde19-Hydroxyprostaglandin E1BrassinolideSerylserineCer(D16:1/20:5(7Z,9Z,11E,13E,17Z)-3Oh(5,6,15))3-Hydroxytetradecanedioic Acid3-HydroxysintaxanthinVeraguensinDg(24:1(15Z)/15:0/0:0)Lysope(24:1(15Z)/0:0)N-Nervonoyl CysteineAlpha-Tocopherol SuccinateCorchoionoside BN-Acetyl-D-GalactosamineGalactosylsphingosine4-Oxo-2-Nonenal(2S)-2-(4-Chloroanilino)Propanoic AcidPalmitoleamide7-[3-Hydroxy-2-(3-Hydroxyoctyl)-5-Oxocyclopentyl]Heptanoic Acid16,16-Dimethyl Prostaglandin E1Acetyl Tributyl CitrateAdenosineN,N-Diisopropylethylamine4-Trimethylammoniobutanoic AcidBax Inhibitor Peptide V5NadPe(14:1(9Z)/14:0)TacrolimusAla-Ala-Ala1,3-BenzodioxolylbutanamineGanoderic Acid ThetaMetoprolol Tartrate3-Rha-Ara-Glcua-Soyasapogenol BDg(20:1(11Z)/18:4(6Z,9Z,12Z,15Z)/0:0)(10R,13S,17S)-17-Hydroxy-13-Methyl-10-[(4-Methylphenyl)Methyl]-17-Prop-1-Ynyl-2,6,7,8,12,14,15,16-Octahydro-1H-Cyclopenta[A]Phenanthren-3-OneNicotinate Adenine Dinucleotide PhosphateDeoxyguanylic AcidPeimineButyryl Trihexyl CitrateDodecanamideL-PhenylalanineCyclic Adp-Ribose3,7-Dihydroxy-25-Methoxycucurbita-5,23-Dien-19-AlErythro-8,10-PentatriacontanediolPhenytoin QuinonePe(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))HumulolYuzu LactonePe(14:0/20:3(6,8,11)-Oh(5))1-Adamantanecarbonyl-Rf-Nh2Leu Pro ArgLauramine OxideCarpaineOrotidylic AcidN-Isobutyloctadeca-Trans-2-Trans-4-DienamidePs(18:2(9Z,12Z)/16:0)DeforolimusArg Cys TyrPhosphonoacetic AcidVal Glu AlaCysteinyl-MethionineElaidic Acid9(Z),11(E)-Conjugated Linoleic AcidPg(Pgd2/I-12:0)DtdpDihydronicotinamide Formycin DinucleotideMethyl 2-Amino-3-PhenylpropanoateD-Mannitol 1-PhosphateFahfa(16:0/13-O-16:0)Lys Cys HisCucurbitacin BMg(0:0/18:1(12Z)-O(9S,10R)/0:0)Hebevinoside IxWithanolide BMannose 1-PhosphateN-Myristoyl Arginine6-Aminocaproic AcidGlycerophosphocholineNonapropylene GlycolPs(16:1(9Z)/18:0)SenegeninAmikacinPc(6 Keto-Pgf1Alpha/2:0)Mmdma5-MethylcytidineTrans-3-Indoleacrylic Acid4-Piperidinone, 1-Hydroxy-2,2,6,6-Tetramethyl-1-MonomyristinPro LeuSerratolTributyl CitrateAcetophenonePpg N11Mg(18:1(9Z)/0:0/0:0)N-Ethyl-N-MethylcathinoneCyclo(D-Trp-D-Asp-Pro-D-Ile-Leu)(+)-Threo-2-Amino-3,4-Dihydroxybutanoic AcidUrea CitrateFrangulanineN,N-DimethylacetamideGlycyrrhetinic AcidEchinocystic AcidStachyflinCystine-GlutamateHis Gln SerOctadeca-10,12-Dienoic AcidSoyasapogenol B 3-O-[A-L-Rhamnosyl-(1->4)-B-D-Galactosyl-(1->4)-B-D-Glucuronide]Ile Gly Leu3-O-Beta-D-Glucosyl-BrassicasterolGinsenoside F3Guanosine 5'-MonophosphateS-Adenosylmethionine9,10-Epoxy-18-Hydroxy-Octadecanoic AcidN-[1-(2-Ethylphenyl)Sulfonylpiperidin-4-Yl]-2-Methoxy-N-(Oxan-4-Yl)Acetamide4-DodecenoylcarnitineGamma-GlutamylalanineMppa (Methyl Pyropheophorbide-A)Glucose-Uridine-C1,5'-Diphosphate5'-Deoxy-5'-MethylthioadenosineLys His GluTyrosine MethylesterTerpestacinPs(15:0/16:0)Beta-CitraurinHericene BDioctyl PhthalatePe(14:0/16:0)[3-[3-(Oxolan-2-Yl)-1,2,4-Triazol-4-Yl]Azetidin-1-Yl]-Pyrimidin-4-YlmethanoneDiethyl Phthalate16-B1-PhytoprostaneBenzophenonePolyoxyethylene 40 MonostearateDg(18:1(12Z)-O(9S,10R)/0:0/I-18:0)N-Methyl-2-(4-Methylphenyl)Sulfonyl-2-Azaspiro[4.4]Nonane-4-CarboxamideIndoline(E)-Ethenoxyimino-Oxido-Pyrrolidin-1-Ylazanium2-Phenylethyl Beta-D-GlucopyranosideAbeadoHovenidulcioside A2LopirazepamStachyoseAdenosine 3',5'-Diphosphate9,12-Octadecadiynoic AcidPipecolic Acid9-Hexadecenoic AcidSebacic AcidN,N,N',N',N'',N''-Hexakis(Methoxymethyl)-1,3,5-Triazine-2,4,6-TriamineVitamin K1ErucamideOctaethyleneglycol Monododecyl EtherGlutamateUrsolic AcidQuassin17-AminogeldanamycinCarnitine4,5-DiphenylimidazoleAustinoneolChenodeoxycholylarginineFloionolic AcidCorchorifatty Acid DL-LysineHeptadecasphinganine5'-Cmp2-Aminobicyclo[2.2.1]Heptane-2-Carboxylic Acid1,2-Ethanediol Monoricinoleate(8R,9R,10S,13S,14S)-13-Methyl-1,4,5,6,7,8,9,10,11,12,14,15,16,17-Tetradecahydrocyclopenta[A]PhenanthreneArginyl-Prolyl-ProlineMs-275Butyl 4-[(2-Ethoxy-4-Oxo-6,7,8,9-Tetrahydroquinolizine-1-Carbonyl)Amino]Benzoate1,3-DicyclohexylureaHexadecanamideN-Methyl-D-Aspartic AcidL-Glyceraldehyde 3-Phosphate16-Hydroxyhexadecanoic AcidRaffinose19-NorandrosteroneDigitoxigenin 3-[Glucosyl-(1->6)-Glucosyl-(1->4)-2,6-Dideoxyribohexoside]TernatinPe-Nme(14:1(9Z)/14:1(9Z))Docosanedioic AcidPs(15:0/15:0)Dioctyl SuccinateCer(D18:2(4E,14Z)/20:5(7Z,9Z,11E,13E,17Z)-3Oh(5,6,15))1,2-Dioleoyl-Sn-Glycerol7-Oxo-N-[3-(1-Propan-2-Ylpiperidin-4-Yl)Oxypropyl]Azepane-2-CarboxamidePg(I-13:0/20:4(8Z,11Z,14Z,17Z)-2Oh(5S,6R))(Z)-11-HexadecenalD-Glucose 6-Phosphate6-(Alpha-D-Glucosaminyl)-1D-Myo-InositolDeoxycholylarginineUdp-4-Dehydro-6-Deoxy-D-Glucose4-[1-[(3,4-Dimethoxyphenyl)Methyl]Pyrrolidin-3-Yl]-1-MethylsulfonylpiperidineNomilinTobramycinTrichloroethanol GlucuronidePyrido(3,4-D)Pyridazine-1,4-Dione, 8-Amino-5-Chloro-2,3-Dihydro-7-Phenyl-1-(2,4-Dimethylphenyl)-3-[(3-Morpholin-4-Yloxolan-3-Yl)Methyl]UreaFosbretabulinfalseRcrR: A Multifunctional Transcriptional Regulator Orchestrating the cariogenic virulence of Streptococcus mutansWe systematically investigated RcrR's contribution to cariogenic virulence using isogenic wild-type, DeltarcrR deletion mutant, and complemented strains. We assessed bacterial growth, biofilm architecture, acid tolerance, and H+-ATPase activity. To delineate the global regulatory network, we integrated untargeted metabolomics with data-independent acquisition (DIA)-based quantitative proteomics. Deletion of rcrR impaired bacterial growth, resulted in a structurally loose biofilm with significantly reduced water-insoluble extracellular polysaccharides (EPS) and extracellular DNA (eDNA), and markedly downregulated the transcription of glucosyltransferase genes gtfB, gtfC, and gtfD. Under acidic conditions (pH 5.5), the DeltarcrR mutant showed substantially reduced acidogenicity and aciduricity, accompanied by significantly diminished H+-ATPase activity. Multi-omics analyses revealed that rcrR deletion depleted critical glycolytic intermediates, most notably fructose-1,6-bisphosphate (FBP), and broadly suppressed the expression of proteins involved in carbohydrate metabolism, ABC transport systems, and quorum sensing pathways. Our findings demonstrate that RcrR functions as a central regulator coordinating energy metabolism, nutrient transport, and cell signaling to drive the full spectrum of S. mutans cariogenic virulence. This identifies RcrR as a promising molecular target for the development of novel preventive and therapeutic strategies against dental 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