MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/m_MTBLS15285_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/s_MTBLS15285.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/a_MTBLS15285_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/A11.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/A13.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/B15.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/A15.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/B17.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/C12.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/B12.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/B14.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/C16.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/A17.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/A12.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/C11.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/C13.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/B11.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/C17.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/A16.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/C15.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285/FILES/targeted_bile_acid_mzML/B13.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15285<p>Bile acids were identified using authentic reference standards and the Metware Database (MWDB). Identification was based on reference-standard retention times, chromatographic peak shapes, and characteristic precursor-to-product ion transitions monitored in multiple reaction monitoring mode. A targeted panel of 82 bile acids was included. Quantification was performed using analyte-specific external calibration curves and internal standards.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phase<p>Chromatographic separation was performed using a SCIEX ExionLC AD UHPLC system equipped with a Waters ACQUITY UPLC HSS T3 C18 column (100 mm × 2.1 mm i.d., 1.8 µm). Mobile phase A consisted of water containing 0.01% acetic acid and 5 mmol/L ammonium acetate, and mobile phase B consisted of acetonitrile containing 0.01% acetic acid. The flow rate was 0.35 mL/min, the column temperature was maintained at 40 °C, and the injection volume was 3 µL. The gradient program was as follows: 5% B at 0 min, 40% B at 1 min, 50% B at 7 min, 75% B at 12 min, 95% B at 14 min, and 5% B at 16 min.</p>Vitamin D deficiency disrupts a host–microbiota bile acid axis in aged mice to drive Faecalibaculum rodentium expansion and intestinal epithelial barrier dysfunction.Xin FengCollege of Food Science and Nutritional Engineering, China Agricultural Universityfecesmass spectrometry assay<p>Fecal samples (20 mg) were homogenized with 248 µL of methanol/acetonitrile (v/v = 2:8) after the addition of 2 µL of an internal standard mixture at 10 µg/mL. The samples were shaken at 2500 r/min for 10 min and incubated at -20 °C for 10 min to precipitate proteins. After centrifugation at 12000 r/min for 10 min at 4 °C, the supernatants were passed through a protein precipitation plate and subjected to LC-MS/MS analysis.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS15285Wenbiao Shi. Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University. wenbiao.shi@cau.edu.cn.Xin Feng. College of Food Science and Nutritional Engineering, China Agricultural University. 15961877791@163.com.<p>Mass spectrometry data were acquired and initially processed using Analyst 1.6.3 software. Chromatographic peaks were integrated and corrected using MultiQuant 3.0.3 with reference to the retention times and peak shapes of authentic standards. Analyte concentrations were calculated from external calibration curves using analyte-to-internal-standard peak area ratios. Bile acid contents in solid samples were expressed as ng/g and calculated using X = c × V / (1000 × m), where c is the concentration calculated from the calibration curve, V is the extraction volume, and m is the sample mass.</p>Faecalibaculum rodentium gavage plus GR-715961877791@163.com<p>Fecal samples were collected from antibiotic-pretreated male C57BL/6J mice assigned to three experimental groups: control, Faecalibaculum rodentium gavage, and Faecalibaculum rodentium gavage combined with the bile salt hydrolase inhibitor GR-7. Samples were collected after the four-week gavage intervention, immediately snap-frozen in liquid nitrogen, and stored at -80 °C until analysis. Six biological samples from each group were included in this dataset.</p>MetabolomicsMetabolomicsAB SCIEX QTRAP 6500+Mus musculustargeted analysisSCIEX ExionLC ADbile acidFaecalibaculum rodentiumexperimental blanktargeted metabolite profilingfecesMetabolomicsAB SCIEX QTRAP 6500+Mus musculustargeted analysisSCIEX ExionLC ADbile acidFaecalibaculum rodentiumexperimental blanktargeted metabolite profilingfeces<p>Mass spectrometric analysis was performed using a SCIEX QTRAP 6500+ triple quadrupole-linear ion trap mass spectrometer equipped with an ESI Turbo Ion-Spray interface. Data were acquired in negative ion mode using scheduled multiple reaction monitoring. The source temperature was 550 °C, the ion spray voltage was -4500 V, and the curtain gas pressure was 35 psi. Declustering potentials and collision energies were optimized individually for each MRM transition. Data acquisition was controlled using Analyst 1.6.3 software.</p>falseTargeted bile acid profiling of Faecalibaculum rodentium-induced bile acid remodeling in antibiotic-treated miceThis study investigated whether Faecalibaculum rodentium remodels the intestinal bile acid pool through bacterial bile salt hydrolase activity in antibiotic-treated male C57BL/6J mice. Mice were assigned to three groups: control, Faecalibaculum rodentium gavage, and Faecalibaculum rodentium gavage combined with the gut-restricted bile salt hydrolase inhibitor GR-7. Targeted bile acid profiling was performed on fecal samples using UPLC-ESI-MS/MS in negative-ion scheduled multiple reaction monitoring mode with an ExionLC AD system coupled to a SCIEX QTRAP 6500+ mass spectrometer. The deposited dataset contains 18 biological samples, with six samples per group, and quantitative measurements from a targeted panel of 82 bile acids.2026-08-072026-08-07MTBLS15285