MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/m_MTBLS15301_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/m_MTBLS15301_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/s_MTBLS15301.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/a_MTBLS15301_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/a_MTBLS15301_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410198_Human_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410193_control_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410202_Human_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410200_Human_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410196_control_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410191_QC01.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410197_Human_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410192_control_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410191_QC01.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410199_Human_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410196_control_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410193_control_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410191_QC02.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410194_control_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410191_QC02.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410191_control_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410194_control_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410198_Human_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410191_control_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410191_QC03.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410200_Human_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410202_Human_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410195_control_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410197_Human_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410199_Human_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410195_control_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/POS/POS_MKG1410201_Human_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410191_QC03.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410201_Human_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301/FILES/RAW_FILES/NEG/NEG_MKG1410192_control_2.rawprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15301<p>Metabolite features were annotated by database searching following LC-MS data preprocessing. Metabolite identification and annotation were performed by comparison against the Human Metabolome Database (HMDB), METLIN, and the self-compiled Majorbio Database (MJDB; Majorbio Bio-Pharm Technology Co., Ltd., Shanghai, China). Identified metabolites were subsequently subjected to biochemical pathway annotation and enrichment analysis using the KEGG database.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using a UHPLC system coupled to a Q Exactive HF-X mass spectrometer. An ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm i.d., 1.8 μm; Waters, USA) was used. The mobile phases consisted of 0.1% formic acid in water/acetonitrile (2:98, v/v) as solvent A and 0.1% formic acid in acetonitrile as solvent B. The flow rate was 0.40 mL/min, the column temperature was maintained at 40°C, and the injection volume was 5 μL.</p>Nano-Kinetic Implant Surface Mediates Osteo-Antibacterial Synergy through Electromagnetic-Mechanical Coupling.Yangmengfan ChenWest China HospitalWhole Organismmass spectrometry assay<p>A 100 μL aliquot of each liquid bacterial suspension sample was transferred into a 1.5 mL centrifuge tube and mixed with 800 μL extraction solution consisting of acetonitrile:methanol (1:1, v/v) containing four internal standards at 0.02 mg/mL, including L-2-chlorophenylalanine. Samples were vortex-mixed for 30 s and subjected to low-temperature ultrasonication for 30 min at 5°C and 40 kHz. Samples were then incubated at -20°C for 30 min to precipitate proteins, followed by centrifugation at 13,000 × g for 15 min at 4°C. The supernatant was collected and dried under nitrogen. The residue was reconstituted in 100 μL acetonitrile:water (1:1, v/v), sonicated for 5 min at 5°C and 40 kHz, and centrifuged at 13,000 × g for 10 min at 4°C. The resulting supernatant was transferred to sample vials for LC-MS/MS analysis. A pooled quality-control (QC) sample was prepared by mixing equal volumes of all study samples and was processed using the same procedure as the analytical samples.</p>Staphylococcus aureushttps://www.ebi.ac.uk/metabolights/MTBLS15301Yangmengfan Chen. West China Hospital. chenyangmengfantj@163.com.<p>Raw LC-MS data were preprocessed using Progenesis QI software (Waters Corporation, Milford, USA). The resulting data matrix contained sample information, metabolite features and mass-spectral response intensities. Internal-standard peaks and known false-positive features, including noise and column-bleed peaks, were removed. Features detected in at least 80% of samples within any experimental group were retained. Missing values were imputed using the minimum value in the data matrix. Signal intensities were normalized using sum normalization to reduce variation arising from sample preparation and instrumental instability. Features in QC samples with a relative standard deviation (RSD) greater than 30% were excluded, and the resulting data were log10 transformed for downstream statistical analysis.</p>Treatmentchenyangmengfantj@163.com<p>Samples consisted of Staphylococcus aureus bacterial suspensions from two groups: laboratory reference S. aureus and periprosthetic implant-associated S. aureus recovered from periprosthetic implant surface lavage fluid. Samples were collected for untargeted LC-MS/MS metabolomic profiling to compare metabolic differences between the laboratory control and periprosthetic implant-associated bacterial groups.</p>MetabolomicsMetabolomicsuntargeted metabolomicsUHPLC system (model not specified)untargeted analysisQ Exactive HF-X Mass Spectrometerbacterial suspensionperiprosthetic joint infectionStaphylococcus aureusWhole OrganismMetabolomicsuntargeted metabolomicsUHPLC system (model not specified)untargeted analysisQ Exactive HF-X Mass Spectrometerbacterial suspensionperiprosthetic joint infectionStaphylococcus aureusWhole Organism<p>Mass spectrometric analysis was performed using a Q Exactive HF-X mass spectrometer equipped with an electrospray ionization (ESI) source. Data were acquired separately in positive and negative ionization modes. The source temperature was set to 400°C, with a sheath gas flow rate of 40 arbitrary units and an auxiliary gas flow rate of 10 arbitrary units. The spray voltage was 3.5 kV in positive ion mode and 2.8 kV in negative ion mode. MS/MS acquisition was performed using stepped normalized collision energies of 20, 40 and 60. Data were acquired in Data Dependent Acquisition (DDA) mode over an m/z range of 70–1050.</p>Pc(6 Keto-Pgf1Alpha/14:0)9H-Purine-9-OlPe(18:1/0:0)RiboflavinP-Coumaraldehyde2,6-Diamino-4-Hydroxy-5-FormamidopyrimidineXestoaminol CProlyl-Tyrosine5-Nitro-2-(3-Phenylpropylamino)Benzoic AcidGpcho(16:0/20:5)Cyclo(Arg-Gly-Asp-D-Phe-Val)4-O-Methyl-12-O-Tetradecanoylphorbol 13-AcetateSchidigeragenin BGlu-LeuN1,N12-DiacetylspermineDiosbulbinoside Fposatirelin3-Amino-2-Methoxynonadec-5-En-4-OlKinetin3-Hydroxypropyl MethacrylateDeoxycholic AcidL-Histidine7,8-DiaminopelargonateChlorobenzeneProlyl-AsparagineN-Acetyl-D-MannosamineLisinopril, Epsilon-Biotinamidocaproyl-L-Homocysteic Acid8-Methylsulfinyloctyl IsothiocyanateL-ProlineLeu-AspDecyl AlcoholGpcho(20:4/16:0)Elymoclavine(+/-)-(Z)-2-(5-Tetradecenyl)CyclobutanoneN1-AcetylspermidineGpcho(20:5/20:4)3-(N-N-Octadecylcarbamoyloxy)-2-Methoxypropyl-2-Thiazolinium Ethyl PhosphateEstradiol 17-O-OctadecanoateCapsicum Annuum Fluorescent Chlorophyll CataboliteIngenol3',4',5'-Trimethoxytricetin 7-[Rhamnosyl-(1->6)-Glucoside]Pilocarpine(1,1-Diamino-4-Sulfamoyloxybutyl) Sulfamate12-O-Retinoylphorbol-13-AcetateGluconapoleiferin(6R)-5,10-MethenyltetrahydrofolateFlavin MononucleotideCyclo(His-Pro)Pz-PeptideSarmentosinCyclo(Leu-Pro)Aegle Marmelos Alkaloid CPg(Pgf1Alpha/I-15:0)EstramustineN-AcetylcadaverineLinatineCefditoren PivoxilOleamideL,L-Cyclo(Leucylprolyl)Cinnamic Acid12-Hete1-Hexadecanoyl-Sn-Glycero-3-PhosphoethanolaminePro-GluAminocarbRitonavir5-Hydroxyindoleacetylglycine4-Trimethylaminobutyraldehyde(Z)-9-Cycloheptadecen-1-One3-Hydroxybutyric AcidTaurine AspartateDihydro-Alpha-Ionone11Beta-HydroxyprogesteroneAllo-Hydroxycitric Acid LactoneN-AcetyltryptamineFusidic AcidTriacanthineJosamycinOxdralazineP-Hydroxyl-EthotoinIfosfamideLys-IleSpisulosineN-Palmitoyl Aspartic AcidCyclo(Phenylalanyl-Prolyl)Methyl 2-Amino-4-MethylpentanoateMethylguanidineL-ThyronineCyclo-Prolylglycine1-Methylguanine1-Oleoyl-2-Palmitoyl-Sn-Glycero-3-PhosphocholineAsparaginyl-ValinePhenethylamine GlucuronideSulforaphane-Cysteine-GlycineHydroxylated LecithinIndole2-Hydroxyadenine4-Methyl-5-ThiazoleethanolIsovaleronitrileN-ArachidonylethanolamineGeranylcitronellolLovastatinS-Allyl-L-CysteineAngoroside C5-Heptyl-2-Furanheptanoic AcidFlavoxanthinIsopentenyladenineGlutaminylcysteineZatebradineHistidylargininePhthalic AnhydrideIsoindolineBorapetosideTempo-MaleimideL-CarnitineN-(2R-Hydroxyhexadecanoyl)-2S-Amino-9-Methyl-4E,8E-Octadecadiene-1,3R-DiolCysteinyl-GlutamineL-NmmaAlpha-IononeThiamineN,N-Diisopropylethylamine4-Trimethylammoniobutanoic AcidDolasetronTomatidineAcetylphosphateCarindoneNadPersinNeplanocin AL-TyrosineGpcho(20:4/18:0)Pg(Pgf1Alpha/18:2(9Z,12Z))ValyltyrosineDimethyl PhthalatePeimineDeoxyguanylic AcidClarithromycinPhe-ProDodecanamideL-Phenylalanine2'-Deoxymugineic AcidPa(Pgj2/I-16:0)TrihexyphenidylN-Isobutyloctadeca-Trans-2-Trans-4-DienamideN-ChlorophenylalanineProguanilSpermidineSphingosineAflatoxin B1Exo-8,9-Epoxide-GshL-Acetopine7,8-Dihydrovomifoliol 9-[Apiosyl-(1->6)-Glucoside]Harmalol2-Hydroxycinnamic Acid2-NaphthylamineThreonylserinePg(Pgf1Alpha/18:2(9Z,11Z))1,4-DiaminobutaneN-Palmitoyl HistidineValeric AcidExatecanDimpMethenamine1,3-Butadiene2-(5,8-Tetradecadienyl)CyclobutanoneTropolone AClomocyclineSpinosin1-(1-(3-Methoxyphenyl)Cyclohexyl)PiperidineN-Iminoethyl-L-OrnithineUridine 5'-DiphosphateIsodeoxycholic AcidNeocuproine4-Coumaric AcidGuanineMammeisinOctadecanamideD-Pyroglutamic AcidQuestiomycin AAcetophenoneMepacrineKetotifen-N-GlucuronideVaccenic AcidIndole-3-CarboxaldehydeNitrobenzeneGlu-ValPisumoside AN-Linoleoyl Histidine5-(3-Pyridyl)-2-HydroxytetrahydrofuranEuscaphic AcidN-Formyl-L-Glutamic AcidN-Acetylneuraminic Acid(1Xi,3Xi)-1,2,3,4-Tetrahydro-1-Methyl-Beta-Carboline-3-Carboxylic AcidLichenin7-MethylguanineN-Acetyl-L-ArginineApigenin 4'-[Feruloyl-(->2)-Glucuronyl-(1->2)-Glucuronide]2-Aminoheptanedioic AcidPgf2A EthanolamideL-HomocarnosineCotinine GlucuronideDocosanamideD-(+)-TryptophanNovobiocinL-Glutathione2-Amino-3-Methyl-1-ButanolDesmediphamEnniatin BFenamic AcidPro-Lys3-Methyl-1-ButylamineH-Phe-D-Met-Arg-Phe-Nh2Imidazole Lactic AcidBetaine6-LactoyltetrahydropterinN-Methyl-L-ProlineHebevinoside ILys-Pro8-HydroxyadenineStachyoseN-Acetyl-S-(2-Carbamoylethyl)-L-CysteineOxymetholone(3Beta,22R,23R,24S)-3,22,23-Trihydroxystigmastan-6-One(2S)-3-Hydroxy-1-[(2S)-Pyrrolidine-2-Carbonyl]Pyrrolidine-2-Carboxylic AcidPipecolic AcidHyaluronan Biosynthesis, Precursor 1Pisumionoside1-Palmitoyl-Sn-Glycero-3-PhosphocholinePro-Ser17-AminogeldanamycinGlutamic AcidPro-Thr2-((3,5-Dihydroxyphenyl)Amino)Acetic Acid2-HeptadecylfuranIsoquinolineMuramic AcidL-LysineGlabrin DCyanidin 3-(6''-Acetylglucoside)Mangiferin1-Phenylpropane-1,2-DioneFarnesiferol ACis-Uvariamicin IvSulfamethoxazole N1-GlucuronideGly-Lys1-MethylhistidineFencloracAnthranilic AcidArg-IleTriethylhexanoinInosine Pyruvate3-KetosphingosineBlebbistatinVinylacetylene3-Acetyl-2,5-DimethylfuranO-Methyl-4-[(2',3',4'-Tri-O-Acetyl-Alpha-L-Rhamnosyloxy)Benzyl]CarbamateCys-Arg-Glu-Lys-AlaThymineActodiginO6-Carboxymethyl-2'-Deoxy-GuanosineN-Acetyl-1,6-DiaminohexaneIsonicotinic Acid22-Hydroxydocosanoic AcidCyclomorusinApcSarcosine4-OxofenretinideOxazineDermatan L-IduronateGallocatechinIle-AspZalcitabine(5Alpha,6Beta,14Alpha,20R,22R)-5,6,14,20,27-Pentahydroxy-1-Oxowith-24-EnolideFlumethasoneTetraethylene GlycolDibutyl SebacateL-HomoserineMg(20:5)Fusarin CDg(Pgj2/A-15:0/0:0)AminodeoxykanamycinPa(6 Keto-Pgf1Alpha/20:2(11Z,14Z))AllobarbitalTrigonellineGpcho(14:0/20:0)Gly-LeuLeu-Lys11-Aminoundecanoic AcidDeoxyloganinArachidic AcidStyreneSalicylanilidePravastatin Lactone2-Aminobutyric Acid4-HydroxyprolineGeranylgeraniol1,2-Benzisothiazol-3(2H)-OneCinncassiol AGamma-Aminobutyric AcidVigabatrinNopalinic AcidIle-ProOctopamineN-Oleoyl HistidineObtusifolinPro-ArgCyclopropanecarboxamide, 1,2,2,3,3-Pentamethyl-Pg(Pgf2Alpha/18:2(9Z,11Z))Glu-GlnCis-MurisolinoneNiacinamideAspirinTenuazonic AcidSerotoninDihydrozeatinMagnoflorineLevocarnitine1-AcetylimidazoleN1,N8-DiacetylspermidineEstrane(2Xi,6Xi)-7-Methyl-3-Methylene-1,2,6,7-OctanetetrolErythrono-1,4-LactonePaullinic AcidLeucylprolineMacrophorin C4-Guanidinobutanoic AcidL-ValineUracilVidarabineCyclo-Dopa 5-O-GlucosideTryptamine3-Indolepropionic Acid5-(12-Heptadecenyl)-1,3-BenzenediolAla-IleVirginiamycin M12-PentadecylfuranCarmofur5-Megastigmen-7-Yne-3,9-Diol 9-GlucosideQuinaldic AcidCreatineOphthalmic AcidCoagulin R 3-Glucoside5-L-Glutamyl-L-Alanine3-Hydroxypicolinic AcidKbt 1585 HydrochlorideOxiglutationeIsoleucylhydroxyprolinePa(Pgf1Alpha/10:0)Pro-AlaN-Acetyl-B-GlucosaminylaminePro-PheMyristic AcidBeclometasone DipropionateDcmpPonasterone AFumonisin Ak13B,6A-Dihydroxy-Alpha-Ionol 9-[Apiosyl-(1->6)-Glucoside]Syringetin5-Hydroxyprimaquine10,11-Dihydro-20-Dihydroxy-Ltb45-(3',4'-Dihydroxyphenyl)-Gamma-Valerolactone-3'-O-GlucuronideBisoprolol1-PiperideinePiromidic AcidSpermineNalpha-Acetyl-L-LysineBrassinolideAlpha-EstradiolPhe-Arg2-Phenyl-5-(5-Methyl-3-Pyridinylmethyl)PyridineGamma-GlutamylphenylalaninePg(Pgf1Alpha/A-15:0)Kynurenic AcidTeroxirone1-(3-Trifluoromethylphenyl)PiperazineNotoginsenoside R2Asparaginyl-Proline1-(2-Aminoacetyl)-N-(4-Nitrophenyl)Pyrrolidine-2-CarboxamideHis-AlaN8-AcetylspermidineAlpha-Linolenic AcidVernolic AcidFolinic AcidFozivudine TidoxilNonadecylresorcinol GlucuronideBeta-D-RibosylnicotinateNemifitideFluocortin ButylSer-ProIndole-3-Lactic AcidMatairesinosideCetefloxacin1-Ethyl-2-PyrrolidinoneTrp-AlaGlu-IleN-Lactoyl-PhenylalanineSilvestrolValganciclovir, (S)-D-Ala-D-Ala2-HydroxyphenethylamineGly-PheVerbascoseInosinePhenytoinLumichromeCalcitroic Acid4-[(2,4-Dihydroxy-3,3-Dimethylbutanoyl)Amino]Butanoic Acid(3B,5A,25R)-3-Hydroxyspirostan-6-One 3-[2-Acetylarabinosyl-(1->6)-Glucoside]GonalEthyl 2-Amino-3-SulfanylpropanoateEthenoadenosineAla-Glu3-Formyl RifamycinP-Tolualdehyde2,5-DimethylbenzaldehydeAconitic AcidCaffeineTyramine6-[(2S,3S,4R,5R)-5-(6-Aminopurin-9-Yl)-3,4-Dihydroxyoxolan-2-Yl]Oxane-2,5-Dione7A,12A-Dihydroxy-3-Oxo-4-Cholenoic AcidMethyl 2-Amino-3-Phenylpropanoate4-DeoxyphysalolactoneCis-15-Octadecenoic AcidGly-Val24,24-DfhvPs(Monome(11,3)/Dime(13,5))L-MethionineSer-AlaDihydrouracilGatifloxacinMelezitoseDihydrodigoxinAquifoliunine EiiiN-Myristoyl Arginine6-Aminocaproic AcidErgothioneine4',5-Dihydroxy-7-Methoxy-6-MethylflavonePhenyl PhosphonothioateTrilostanePyrazofurinTyr-ProS-(Pga2)-GlutathioneZeaxanthinOlitorin6-O-OleuropeoylsucroseAcevaltrateN,N'-Bis(G-Glutamyl)-3,3'-(1,2-Propylenedithio)DialanineN-[(3A,5B,7A,12A)-3,12-Dihydroxy-24-Oxo-7-(Sulfooxy)Cholan-24-Yl]-GlycineBemcentinibHeptaethylene Glycol Monododecyl EtherMethionine SulfoxideVeratrinePenitrem AAlanine LactateAsp-IleCytochlorAclacinomycin S5-Butyl-5-Cycloheptenylbarbituric AcidN-AcetylhistidineOctadeca-10,12-Dienoic AcidSoyasapogenol B 3-O-[A-L-Rhamnosyl-(1->4)-B-D-Galactosyl-(1->4)-B-D-Glucuronide]Physagulin GN-Acetylputrescine2-PhenylethylamineAbscisic AcidPhysagulin APro-ValCholineN-Acetyl-Glucosamine 1-PhosphateS-Ethyl-L-CysteineAsteltoxin2,3-DihydrobenzofuranN-Acetyl-1-MethylhistidineDiphenylurea9-Octadecynoic AcidGlu-LysMonocrotaline2-Amino-2-Methylpropan-1-OlCrosatoside AAmrinoneHexadecenal6'-O-FormylmarminL-Isoleucine5-Hydroxyindolin-2-One Sulfate2-(4-Hydroxyphenylazo)Benzoic AcidVal-SerIndolineN-CarbamoylsarcosineGpcho(18:0/20:5)N-Stearoyl Histidine(2S,3R)-2-Amino-3-[(2S)-2-Amino-3-Hydroxypropanoyl]Oxybutanoic AcidNandroloneHovenidulcioside A1N,N-DimethylarginineHomodolicholideGlycocholic Acid 3-Sulfate1-(Beta-D-Ribofuranosyl)-1,4-DihydronicotinamideErucamidePentanamideCefoxitinEtiracetamHistamineNicotinic Acid2-(1,2,3,4-Tetrahydroxybutyl)-6-(2,3,4-Trihydroxybutyl)PyrazineGpcho(18:2/18:2)Ethyl CelluloseAdenosine, 8-(Butylamino)-N-Cyclopentyl-Tetrahydrofurfuryl AcetateHeptadecasphinganine20-Cooh-Leukotriene E4Thr-ProKaempferol 3-Arabinofuranoside 7-RhamnofuranosideTropantiol6,8-DihydroxypurineHexadecanamideAnsamitocin P-3Gamma-Glutamyl-2-Aminobutyric AcidRhodamine 6G2-Chlorooctadecanoic Acid8-PentanoylneosolaniolDigitoxigenin 3-[Glucosyl-(1->6)-Glucosyl-(1->4)-2,6-Dideoxyribohexoside]PiperidinePro-ProN2-Succinyl-L-OrnithineKb 2Paramethasone AcetateLpc(18:0)Hydroxytyrosol13,16-Docosadienoic AcidPhenylacetic Acid6,7,4'-Trihydroxyisoflavone4-ChlorophenylImidazoleacetic Acid1-Methyl-2-PyrrolidinoneBiopterinDaidzeinBiotinyl-5'-AmpPyridoxal 5-PhosphatefalseNano-Kinetic Implant Surface Mediates Osteo-Antibacterial Synergy through Electromagnetic-Mechanical CouplingThis untargeted LC-MS/MS metabolomics study investigates metabolic differences between laboratory reference Staphylococcus aureus and periprosthetic implant-associated Staphylococcus aureus in bacterial suspension samples associated with implant surface lavage. The metabolomics dataset forms part of a study evaluating a remotely activated movable magnetic topographic surface (MMTS) designed to disrupt bacterial biofilms under an external electromagnetic field (EMF). Metabolites were profiled in both positive and negative electrospray ionization modes to characterize differential metabolic features associated with the two bacterial groups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