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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/m_MTBLS15334_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/s_MTBLS15334.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/a_MTBLS15334_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PA3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/Ctrl4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PS4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/Ctrl2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PS2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PA5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/Ctrl5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/Ctrl3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PA2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PS5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PA1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PS1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PA4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/PS3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334/FILES/RAW_FILES/Ctrl1.zipprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15334After analyzing all the target substances' chromatographic peaks, the integrals were calculated. A standard curve was constructed using the standards to perform quantitative analysis, and the on-machine detection concentration was obtained. Further, based on the sample sampling volume, the specific content data of the target substances in the samples were calculated.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseUsing a Kinetex C8 chromatographic column (100Å, 2.6 µm, 2.1 mm × 100 mm), with a flow rate of 0.3 mL/min, column temperature of 40 ℃, auto sampler at 4 ℃, and injection volume of 1 μL. The mobile phase A is 0.1% formic acid water, and the mobile phase B is 50% methanol-isopropanol (containing 0.1% formic acid). The gradient of the mobile phase is as follows: 0-1 min: A/B is 80:20 (V/V); 6Min: A/B ratio is 60:40 (volume/volume); for 9-10 minutes, the ratio becomes 80:20 (volume/volume).Targeted metabolomics of mouse feces with sodium propionate supplementation.jiang feiXiangya Hospital, Central South Universityfecesmass spectrometry assayThe entire experiment was conducted on ice.2. Take 20 mg of solid sample and add it to a 2 mL centrifuge tube. Add two steel balls and 800 μL of extraction agent (containing an internal standard). Vortex for 60 seconds.3. Put it in the tissue grinder and grind at 55 Hz for 60 seconds.4. Repeat this once.5. Centrifuge at 4000g for 10 minutes at 10°C.6. Take 40 μL of the supernatant, add 20 μL of 200 mM 3-NPH and mix well.7. Add 20 μL of 120 mM (EDC)·HCl-6% pyridine solution and mix well.8. In a constant temperature metal shaker at 40°C, vibrate at 1200 rpm for 30 minutes.9. After the reaction, place it on ice to cool for 3 minutes.10. Centrifuge at 12000g for 10 minutes at 4°C.11. Take the supernatant, add 150 μL of 0.1% formic acid water (v/v) to make up to 200 μL, vortex and mix well for 60 seconds.12. Centrifuge at 12000g for 10 minutes at 4°C.13. Take the supernatant, pass it through a 0.22 μm filter membrane. Transfer the filtrate to a bottle, with the volume of the bottle not less than 50 μL per bottle.Mus nusculushttps://www.ebi.ac.uk/metabolights/MTBLS15334jiang fei. Xiangya Hospital, Central South University. feijiangfj@csu.edu.cn.The data were processed using the quantitative software OS (version: 4.7, SCIEX) to obtain the mass spectrometry analysis data of different samples. Sodium propionatefeijiangfj@csu.edu.cnmouse feces. ctrl, ps, sodium propiionate.MetabolomicsMus nusculusMetabolomicsProteoWizard msconvertAB SCIEX Triple Quad 6500+colorectal cancertargeted analysisexperimental blankSCIEXAB SCIEXfecesMus nusculusMetabolomicsProteoWizard msconvertAB SCIEX Triple Quad 6500+colorectal cancertargeted analysisexperimental blankSCIEXAB SCIEXfecesThe SCIEX Citrine Triple Quad TM mass spectrometer uses the software OS (version: 4.7, SCIEX), and operates in the negative ESI (Electrospray Ionization) mode, employing the multiple reaction monitoring (MRM) data acquisition method. Ion source parameters: the negative mode voltage of the ion spray (Ionspray, IS) is -4500V, the spray gas (GS1) and auxiliary heating gas (GS2) are 50psi and 50 psi respectively, and the desolvation gas temperature is 550℃; the gas curtain gas (Curtain Gas, CUR) is 30 psi, and the collision gas (Collision Gas, CAD) is 10.falseTargeted metabolomics of mouse feces with sodium propionate supplementationTargeted metabolomics of mouse feces with sodium propionate supplementation2027-08-132026-08-13MTBLS15334