After analyzing all the target substances' chromatographic peaks, the integrals were calculated. A standard curve was constructed using the standards to perform quantitative analysis, and the on-machine detection concentration was obtained. Further, based on the sample sampling volume, the specific content data of the target substances in the samples were calculated.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase"],"chromatography_protocol":["Using a Kinetex C8 column (100 Å, 2.6 µm, 2.1 mm × 100 mm), flow rate of 0.3 mL/min, column temperature of 40 °C, autosampler temperature of 4 °C, and injection volume of 1 μL. Mobile phase A was 0.1% formic acid in water, and mobile phase B was 50% methanol-isopropanol containing 0.1% formic acid. The gradient program was as follows: 0–1 min: A/B = 80:20 (v/v). 6
min: A/B = 60:40 (v/v); 9–10 min: A/B = 80:20 (v/v).
"],"publication":["Targeted metabolomics of mouse feces with F. rodentium supplementation."],"submitter_name":["jiang fei"],"submitter_affiliation":["Xiangya Hospital, Central South University"],"organism_part":["feces"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["1. Perform all steps on ice throughout the experiment;
2. Transfer 20 mg of solid sample into a 2 mL centrifuge tube, add two steel beads, and add 800 μL extraction solution (containing internal standard). Vortex for 60 seconds;
3. Place in tissue grinder and grind at 55 Hz for 60 seconds;
4. Repeat once;
5. Centrifuge at 4000 g for 10 minutes at 10°C;
6. Take 40 μL supernatant and mix with 20 μL of 200 mM 3-NPH;
7. Add 20 μL of 120 mM EDC·HCl–6% pyridine solution and mix thoroughly;
8. Incubate at 40°C in a constant-temperature metal bath, shaking at 1200 rpm for 30 minutes;
9. After reaction, cool on ice for 3 minutes;
10. Centrifuge at 12000 g for 10 minutes at 4°C;
11. Take 50 μL supernatant and dilute to 200 μL with 150 μL formic acid solution (v/v, containing 0.1% formic acid), vortex for 60 seconds;
12. Centrifuge at 12000 g for 10 minutes at 4°C;
13. Filter the supernatant through a 0.22 μm filter membrane, collect the filtrate, and aliquot into bottles with a minimum volume of 50 μL per bottle.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15335"],"author":["jiang fei. Xiangya Hospital, Central South University. feijiangfj@csu.edu.cn."],"data_transformation_protocol":["The data were processed using the quantitative software OS (version: 4.7, SCIEX) to obtain the mass spectrometry analysis data of different samples.
"],"study_factor":["CFU/mL"],"submitter_email":["feijiangfj@csu.edu.cn"],"sample_collection_protocol":["mouse feces. ctrl, ps F. rodentium.store -80 degree
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","ProteoWizard msconvert","Mus musculus","colorectal cancer","targeted analysis","experimental blank","AB SCIEX QTRAP 6500","SCIEX","AB SCIEX","feces"],"curator_keywords":["Metabolomics","ProteoWizard msconvert","Mus musculus","colorectal cancer","targeted analysis","experimental blank","AB SCIEX QTRAP 6500","SCIEX","AB SCIEX","feces"],"mass_spectrometry_protocol":["The SCIEX Citrine Triple Quad™ mass spectrometer was operated using the OS software (version: 4.7, SCIEX) in ESI (Electrospray Ionization) negative mode with Multiple Reaction Monitoring (MRM) data acquisition mode. Ion source parameters were as follows: ion spray voltage (IS) set to -4500 V in negative mode; gas1 (GS1) and gas2 (GS2) flow rates at 50 psi and 50 psi respectively; desolvation temperature at 550°C; curtain gas (CUR) at 30 psi; and collision gas (CAD) at 10.
"],"additional_accession":[]},"is_claimable":false,"name":"Targeted metabolomics of mouse feces with F. rodentium supplementation","description":"Targeted metabolomics of mouse feces with F. rodentium supplementation","dates":{"publication":"2027-08-13","submission":"2026-08-13"},"accession":"MTBLS15335","cross_references":{}}