Targeted metabolite quantification and identification were performed using the automation analysis software BioBud (v2.0.4).
For further biological interpretation and pathway analysis, the identified targeted metabolites were mapped and annotated using authoritative reference databases including HMDB, PubChem, and KEGG.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - hilic"],"chromatography_protocol":["Liquid chromatography separation was performed using an Agilent 1290 Infinity II UHPLC system.
For the reversed-phase (RP) system, an ACQUITY UPLC BEH C18 Column (1.7 μm, 2.1 mm × 150 mm) was utilized.
The RP mobile phase A was 0.1% formic acid in water, and mobile phase B was water/methanol (5:95) containing 10 mmol/L ammonium formate.
For the HILIC system, an Atlantis Premier BEH Z-HILIC Column (1.7 μm, 2.1 mm × 150 mm) was used.
The HILIC mobile phase A consisted of 10% acetonitrile in water with 10 mmol/L ammonium acetate, and mobile phase B was water/acetonitrile (10:90) with 10 mmol/L ammonium acetate.
The auto-sampler temperature was maintained at 6 °C, and the injection volume was set between 1 μL and 2 μL.
"],"publication":["Metabolic plasticity of lipid storage and utilization governs fungal persister formation within macrophages."],"submitter_affiliation":["Peking union medical college hospital"],"submitter_name":["Yi Li"],"organism_part":["synthetic","Cell"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Metabolites were extracted by adding 500 μL of extraction agent (methanol:water = 3:1, containing internal standards) to 25 mg of the cell pellet sample.
Two 3.2 mm beads were added, and the mixture was homogenized at 40 Hz for 4 minutes.
The samples were then sonicated in an ice-water bath for 5 minutes, and this homogenization-sonication cycle was repeated three times.
Following incubation at -40 °C for 1 hour, the samples were centrifuged at 12000 rpm and 4 °C for 15 minutes.
A 400 μL aliquot of the supernatant was transferred and dried under vacuum.
The residue was reconstituted in 200 μL of methanol:acetonitrile:water (1:1:2, v/v/v), filtered, and the liquid was transferred to an autosampler vial for analysis.
A series of calibration standard solutions and QC samples were also prepared for the quantitative assay and method validation.
"],"organism":["synthetic","Candida glabrata"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15358"],"author":["Su Yanyu.","Li Yingxing.","Yi Li. Peking Union Medical College Hospital. liyi67@pumch.cn."],"data_transformation_protocol":["Raw data files generated by LC-MS/MS were processed using the SCIEX Analyst Work Station Software (version 1.7.3).
During data preprocessing, metabolites were filtered to retain only those with no more than 50% missing values in a single group or across all groups.
Missing values were then imputed using the minimum value multiplied by a random number between 0.1 and 0.5.
Prior to statistical and multivariate pattern recognition analyses, the data were scaled and logarithmically transformed using an in-house R script and the ropls package (v1.34.0).
"],"study_factor":["Treatment","Strain"],"submitter_email":["liyi67@pumch.cn"],"sample_collection_protocol":["Three experimental groups of Candida glabrata strains were prepared for targeted metabolomics analysis: wild-type (WT), the ΔASN2 mutant, and the asparagine-supplemented ΔASN2 mutant (ASN2R). Fungal cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) for 3 hours to induce metabolic responses. For the ASN2R group, the DMEM culture medium was additionally supplemented with 1 mM L-asparagine. Each of the three conditions consisted of 6 independent biological replicates. Following the 3-hour incubation period, the fungal cells were rapidly harvested by centrifugation, washed with cold PBS to halt metabolism, and the resulting cell pellets were immediately snap-frozen in liquid nitrogen and stored at -80°C until subsequent metabolite extraction.
"],"omics_type":["Metabolomics"],"study_design":["synthetic","Metabolomics","AB SCIEX Triple Quad 6500+","targeted analysis","liquid chromatography-mass spectrometry","Agilent 1290 Infinity II UHPLC","Candida glabrata","experimental blank","targeted metabolite profiling","Cell"],"curator_keywords":["synthetic","Metabolomics","AB SCIEX Triple Quad 6500+","targeted analysis","liquid chromatography-mass spectrometry","Agilent 1290 Infinity II UHPLC","Candida glabrata","experimental blank","targeted metabolite profiling","Cell"],"mass_spectrometry_protocol":["Mass spectrometry data were acquired using an AB Sciex Triple Quad 6500+ mass spectrometer equipped with an IonDrive Turbo V ESI ion source.
The data acquisition was conducted in Multiple Reaction Monitoring (MRM) mode.
The specific ion source parameters were set as follows: IonSpray Voltage at +5500 V / -4500 V to accommodate both positive and negative ionization modes.
Curtain Gas was set at 35 psi, Temperature at 400 °C, Ion Source Gas 1 at 50 psi, and Ion Source Gas 2 at 50 psi.
"],"metabolite_name":["D-Fructose","L-Phenylalanine","Glycolic Acid","D-Ribulose 5-phosphate","Glucaric acid","Uridine-5'-diphosphate","Guanosine-5'-triphosphate","L-Argininosuccinic Acid","Mannose 6-phosphate","Adenosine 5'-diphosphoribose","Thiamine pyrophosphate","Thymidine-5'-phosphate","L-Threonine","Uridine diphosphate glucose","Ribose-5-Phosphate","L-Histidine","alpha-Ketoglutaric acid","2-Oxobutanoic acid","L-Leucine","3',5'-cyclic AMP","L-Citrulline","alpha-Ketoisovaleric acid","Guanosine Diphosphate-Fucose","Nicotinamide riboside","Vanillylmandelic acid","Glucose-1-phosphate","L-Proline","L-Methionine","D-Glucuronic acid","Flavin mononucleotide","Guanosine Diphosphate-Mannose","Uridine-5'-monophosphate","Nicotinamide","Trehalose-6-phosphate","Glucosamine","N-Acetylglucosamine 6-phosphate","D-Glucose-6-phosphate","2-Picolinic acid","Pyruvic acid","Glycine","Guanine","Gamma-Aminobutyric Acid","L-Asparagine","Ribulose 1,5-bisphosphate","Adenosine-5'-Monophosphate","Mevalonic acid","Pyroglutamic acid","L-Tryptophan","Coenzyme A","Glucosamine 6-phosphate","Glutathione","Creatinine","Fructose-6-phosphate","Phosphoenolpyruvic acid","6-Phosphogluconic acid","Citric acid","S-Adenosylmethionine","L-Glutamine","Adenosine-5'-diphosphate","Uridine diphosphate glucuronic acid","Nicotinamide adenine dinucleotide phosphate","Choline","Lipoamide","L-Valine","Uracil","Succinyl-CoA","Dihydronicotinamide adenine dinucleotide phosphate","Guanosine","Glyceraldehyde 3-phosphate","Creatine","L-Ornithine","L-Isoleucine","S-Adenosyl-homocysteine","Erythrose 4-phosphate","Orotic acid","Itaconic acid","Glycerophosphoric acid","Methylmalonic acid","Betaine","Maleic acid","L-Alanine","Malic acid","Succinic semialdehyde","Cytosine","Glyceric acid","3-Phosphoglyceric acid","Flavin adenine dinucleotide","6-phosphogluconolactone","2,3-Diphosphoglyceric acid","Gluconic acid","N-Acetyl-Neuraminic Acid","L-Cystine","3-Ureidopropionic acid","Nicotinamide adenine dinucleotid","2-Phosphoglyceric acid","Oxalic acid","Oxalacetic acid","Fructose-1,6-diphosphate","Uridine","Succinic acid","Hypoxanthine","L-Aspartic acid","L-Lysine","Nicotinamide mononucleotide","2'-Deoxyadenosine-5'-monophosphate","Guanosine-5'-Monophosphate","L-Arginine","D-Glucose","2-Oxoadipic acid","Hippuric acid","Guanosine-5'-diphosphate","L-Cysteic acid","4-Hydroxyphenylpyruvic acid","Isocitric acid","L-Cysteine","UDP-N-acetylglucosamine","2-Aminobenzoic acid","L-Lactic acid","Quinolinic acid","2-Hydroxybutyric acid","cis-Aconitic acid","Deoxyuridine monophosphate","L-Glutamic acid","Adenosine","Adenosine-5'-triphosphate","Indole-3-acetic acid","N,N-Dimethylglycine","Adenine","Sedoheptulose 7-phosphate","1,4-Dihydronicotinamide adenine dinucleotide","Acetyl-CoA","Phosphoribosyl pyrophosphate","3-Hydroxypropionic acid","2-Hydroxyglutaric acid","Homogentisic acid","N-Acetylglucosamine 1-phosphate","D-Ribose","L-Tyrosine","Fumaric acid","Citraconic acid","L-Serine","Inosine","Trans-Aconitic acid","3-Hydroxybutyric acid","Glutaryl-CoA","Glutathione Disulfide"],"additional_accession":[]},"is_claimable":false,"name":"targeted metabolomic analysis of carbon and energy intermediates in WT Candida glabrata, ΔASN2_Cgl, and asparagine-supplemented ΔASN2_Cgl cells","description":"Targeted metabolomic analysis of carbon and energy intermediates in WT Candida glabrata, ΔASN2_Cgl, and asparagine-supplemented ΔASN2_Cgl cells","dates":{"publication":"2026-10-07","submission":"2026-08-16"},"accession":"MTBLS15358","cross_references":{"HMDB":["HMDB0000123","HMDB0000161","HMDB0000097","HMDB0000112","HMDB0000630","HMDB0000562","HMDB0000162","HMDB0000043","HMDB0000883","HMDB0000167","HMDB0000064","HMDB0000214","HMDB0000168","HMDB0000191","HMDB0000034","HMDB0000696","HMDB0000132","HMDB0000517","HMDB0000904","HMDB0000158","HMDB0000052","HMDB0001185","HMDB0003337","HMDB0000902","HMDB0000115","HMDB0000190","HMDB0000134","HMDB0000254","HMDB0000202","HMDB0000267","HMDB0000156","HMDB0000157","HMDB0000208","HMDB0000232","HMDB0000263","HMDB0000072","HMDB0000625","HMDB0001321","HMDB0000663","HMDB0001548","HMDB0000296","HMDB0001401","HMDB0000124","HMDB0001316","HMDB0258206","HMDB0001058","HMDB0001341","HMDB0000538","HMDB0001273","HMDB0059655","HMDB0000011","HMDB0001259","HMDB0000005","HMDB0000092","HMDB0000187","HMDB0000176","HMDB0000226","HMDB0000225","HMDB0000130","HMDB0001514","HMDB0000660","HMDB0000618","HMDB0001254","HMDB0001367","HMDB0001586","HMDB0001294","HMDB0002817","HMDB0001409","HMDB0001227","HMDB0000905","HMDB0000045","HMDB0000939","HMDB0000295","HMDB0001178","HMDB0000935","HMDB0252660","HMDB0001163","HMDB0000290","HMDB0000217","HMDB0001206","HMDB0001339","HMDB0000058","HMDB0001397","HMDB0001201","HMDB0000125","HMDB0000197","HMDB0000229","HMDB0000221","HMDB0001022","HMDB0000700","HMDB0000807","HMDB0000362","HMDB0000026","HMDB0341410","HMDB0000283","HMDB0304322","HMDB0002092","HMDB0000148","HMDB0001127","HMDB0000192","HMDB0000122","HMDB0001078","HMDB0000288","HMDB0000127","HMDB0000139","HMDB0001423","HMDB0000230","HMDB0001124","HMDB0000286","HMDB0001372","HMDB0000126","HMDB0000855","HMDB0000280","HMDB0002757","HMDB0000958","HMDB0000574","HMDB0001406","HMDB0000687","HMDB0000172","HMDB0000182","HMDB0000641","HMDB0000177","HMDB0000159","HMDB0000050","HMDB0001487","HMDB0001248","HMDB0000243","HMDB0000300","HMDB0001123","HMDB0000193","HMDB0000094","HMDB0000195","HMDB0001520","HMDB0000019","HMDB0002243","HMDB0000227","HMDB0000707","HMDB0000962","HMDB0000929","HMDB0002329","HMDB0001112","HMDB0000223","HMDB0000634","HMDB0000291","HMDB0000714","HMDB0000133"]}}