MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/m_MTBLS15358_LC-MS_alternating_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/a_MTBLS15358_LC-MS_alternating_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/s_MTBLS15358.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2R_5.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-13.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-10.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-05.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-02.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/WT_4.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2_4.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-08.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2R_2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/WT_1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2_1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/WT_6.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2_2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2R_1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-14.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-09.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/WT_3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-11.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2_5.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-06.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2R_4.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-03.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/WT_2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2_6.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2_3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/WT_5.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-07.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2R_3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Asn2R_6.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-15.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-12.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-01.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358/FILES/Cal-04.mzMLprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15358<p>Targeted metabolite quantification and identification were performed using the automation analysis software BioBud (v2.0.4). </p><p>For further biological interpretation and pathway analysis, the identified targeted metabolites were mapped and annotated using authoritative reference databases including HMDB, PubChem, and KEGG.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - hilic<p>Liquid chromatography separation was performed using an Agilent 1290 Infinity II UHPLC system. </p><p>For the reversed-phase (RP) system, an ACQUITY UPLC BEH C18 Column (1.7 μm, 2.1 mm × 150 mm) was utilized. </p><p>The RP mobile phase A was 0.1% formic acid in water, and mobile phase B was water/methanol (5:95) containing 10 mmol/L ammonium formate. </p><p>For the HILIC system, an Atlantis Premier BEH Z-HILIC Column (1.7 μm, 2.1 mm × 150 mm) was used. </p><p>The HILIC mobile phase A consisted of 10% acetonitrile in water with 10 mmol/L ammonium acetate, and mobile phase B was water/acetonitrile (10:90) with 10 mmol/L ammonium acetate. </p><p>The auto-sampler temperature was maintained at 6 °C, and the injection volume was set between 1 μL and 2 μL.</p>Metabolic plasticity of lipid storage and utilization governs fungal persister formation within macrophages.Peking union medical college hospitalYi LisyntheticCellmass spectrometry assay<p>Metabolites were extracted by adding 500 μL of extraction agent (methanol:water = 3:1, containing internal standards) to 25 mg of the cell pellet sample. </p><p>Two 3.2 mm beads were added, and the mixture was homogenized at 40 Hz for 4 minutes. </p><p>The samples were then sonicated in an ice-water bath for 5 minutes, and this homogenization-sonication cycle was repeated three times. </p><p>Following incubation at -40 °C for 1 hour, the samples were centrifuged at 12000 rpm and 4 °C for 15 minutes. </p><p>A 400 μL aliquot of the supernatant was transferred and dried under vacuum. </p><p>The residue was reconstituted in 200 μL of methanol:acetonitrile:water (1:1:2, v/v/v), filtered, and the liquid was transferred to an autosampler vial for analysis. </p><p>A series of calibration standard solutions and QC samples were also prepared for the quantitative assay and method validation.</p>syntheticCandida glabratahttps://www.ebi.ac.uk/metabolights/MTBLS15358Su Yanyu.Li Yingxing.Yi Li. Peking Union Medical College Hospital. liyi67@pumch.cn.<p>Raw data files generated by LC-MS/MS were processed using the SCIEX Analyst Work Station Software (version 1.7.3). </p><p>During data preprocessing, metabolites were filtered to retain only those with no more than 50% missing values in a single group or across all groups. </p><p>Missing values were then imputed using the minimum value multiplied by a random number between 0.1 and 0.5. </p><p>Prior to statistical and multivariate pattern recognition analyses, the data were scaled and logarithmically transformed using an in-house R script and the ropls package (v1.34.0).</p>TreatmentStrainliyi67@pumch.cn<p>Three experimental groups of <em>Candida glabrata</em> strains were prepared for targeted metabolomics analysis: wild-type (WT), the ΔASN2 mutant, and the asparagine-supplemented ΔASN2 mutant (ASN2R). Fungal cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) for 3 hours to induce metabolic responses. For the ASN2R group, the DMEM culture medium was additionally supplemented with 1 mM L-asparagine. Each of the three conditions consisted of 6 independent biological replicates. Following the 3-hour incubation period, the fungal cells were rapidly harvested by centrifugation, washed with cold PBS to halt metabolism, and the resulting cell pellets were immediately snap-frozen in liquid nitrogen and stored at -80°C until subsequent metabolite extraction.</p>MetabolomicssyntheticMetabolomicsAB SCIEX Triple Quad 6500+targeted analysisliquid chromatography-mass spectrometryAgilent 1290 Infinity II UHPLCCandida glabrataexperimental blanktargeted metabolite profilingCellsyntheticMetabolomicsAB SCIEX Triple Quad 6500+targeted analysisliquid chromatography-mass spectrometryAgilent 1290 Infinity II UHPLCCandida glabrataexperimental blanktargeted metabolite profilingCell<p>Mass spectrometry data were acquired using an AB Sciex Triple Quad 6500+ mass spectrometer equipped with an IonDrive Turbo V ESI ion source. </p><p>The data acquisition was conducted in Multiple Reaction Monitoring (MRM) mode. </p><p>The specific ion source parameters were set as follows: IonSpray Voltage at +5500 V / -4500 V to accommodate both positive and negative ionization modes. </p><p>Curtain Gas was set at 35 psi, Temperature at 400 °C, Ion Source Gas 1 at 50 psi, and Ion Source Gas 2 at 50 psi.</p>D-FructoseL-PhenylalanineGlycolic AcidD-Ribulose 5-phosphateGlucaric acidUridine-5'-diphosphateGuanosine-5'-triphosphateL-Argininosuccinic AcidMannose 6-phosphateAdenosine 5'-diphosphoriboseThiamine pyrophosphateThymidine-5'-phosphateL-ThreonineUridine diphosphate glucoseRibose-5-PhosphateL-Histidinealpha-Ketoglutaric acid2-Oxobutanoic acidL-Leucine3',5'-cyclic AMPL-Citrullinealpha-Ketoisovaleric acidGuanosine Diphosphate-FucoseNicotinamide ribosideVanillylmandelic acidGlucose-1-phosphateL-ProlineL-MethionineD-Glucuronic acidFlavin mononucleotideGuanosine Diphosphate-MannoseUridine-5'-monophosphateNicotinamideTrehalose-6-phosphateGlucosamineN-Acetylglucosamine 6-phosphateD-Glucose-6-phosphate2-Picolinic acidPyruvic acidGlycineGuanineGamma-Aminobutyric AcidL-AsparagineRibulose 1,5-bisphosphateAdenosine-5'-MonophosphateMevalonic acidPyroglutamic acidL-TryptophanCoenzyme AGlucosamine 6-phosphateGlutathioneCreatinineFructose-6-phosphatePhosphoenolpyruvic acid6-Phosphogluconic acidCitric acidS-AdenosylmethionineL-GlutamineAdenosine-5'-diphosphateUridine diphosphate glucuronic acidNicotinamide adenine dinucleotide phosphateCholineLipoamideL-ValineUracilSuccinyl-CoADihydronicotinamide adenine dinucleotide phosphateGuanosineGlyceraldehyde 3-phosphateCreatineL-OrnithineL-IsoleucineS-Adenosyl-homocysteineErythrose 4-phosphateOrotic acidItaconic acidGlycerophosphoric acidMethylmalonic acidBetaineMaleic acidL-AlanineMalic acidSuccinic semialdehydeCytosineGlyceric acid3-Phosphoglyceric acidFlavin adenine dinucleotide6-phosphogluconolactone2,3-Diphosphoglyceric acidGluconic acidN-Acetyl-Neuraminic AcidL-Cystine3-Ureidopropionic acidNicotinamide adenine dinucleotid2-Phosphoglyceric acidOxalic acidOxalacetic acidFructose-1,6-diphosphateUridineSuccinic acidHypoxanthineL-Aspartic acidL-LysineNicotinamide mononucleotide2'-Deoxyadenosine-5'-monophosphateGuanosine-5'-MonophosphateL-ArginineD-Glucose2-Oxoadipic acidHippuric acidGuanosine-5'-diphosphateL-Cysteic acid4-Hydroxyphenylpyruvic acidIsocitric acidL-CysteineUDP-N-acetylglucosamine2-Aminobenzoic acidL-Lactic acidQuinolinic acid2-Hydroxybutyric acidcis-Aconitic acidDeoxyuridine monophosphateL-Glutamic acidAdenosineAdenosine-5'-triphosphateIndole-3-acetic acidN,N-DimethylglycineAdenineSedoheptulose 7-phosphate1,4-Dihydronicotinamide adenine dinucleotideAcetyl-CoAPhosphoribosyl pyrophosphate3-Hydroxypropionic acid2-Hydroxyglutaric acidHomogentisic acidN-Acetylglucosamine 1-phosphateD-RiboseL-TyrosineFumaric acidCitraconic acidL-SerineInosineTrans-Aconitic acid3-Hydroxybutyric acidGlutaryl-CoAGlutathione Disulfidefalsetargeted metabolomic analysis of carbon and energy intermediates in WT Candida glabrata, ΔASN2_Cgl, and asparagine-supplemented ΔASN2_Cgl cellsTargeted metabolomic analysis of carbon and energy intermediates in WT Candida glabrata, ΔASN2_Cgl, and asparagine-supplemented ΔASN2_Cgl cells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