MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/m_MTBLS15361_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/m_MTBLS15361_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/a_MTBLS15361_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/a_MTBLS15361_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/s_MTBLS15361.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_iv_2_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_iv_1_NEG.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_PBS_4_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_PBS_3_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_iv_2_NEG.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_iv_3_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_iv_4_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_iv_3_NEG.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_PBS_1_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_iv_4_NEG.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_PBS_4_NEG.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_PBS_3_NEG.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_PBS_2_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_PBS_2_NEG.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_POS/all_iv_1_POS.mzXMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361/FILES/DERIVED_FILES/LCMS_NEG/all_PBS_1_NEG.mzXMLprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15361<p>Metabolites were annotated by matching accurate mass, retention time and MS/MS fragmentation spectra against the Novogene in-house spectral library. Metabolite annotations were further linked to the HMDB, KEGG, LIPID MAPS and PubChem databases. Metabolite identification confidence was assigned according to the available MS1, MS/MS and retention-time matching information.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using a Thermo Fisher Scientific Vanquish UHPLC system equipped with a Thermo Scientific Hypersil GOLD C18 column (100 × 2.1 mm, 1.9 µm). The column temperature was maintained at 40°C and the flow rate was 0.2 mL/min. Mobile phase A was 0.1% formic acid in water and mobile phase B was methanol. The gradient was: 0-1.5 min, 98% A and 2% B; 3 min, 15% A and 85% B; 10 min, 0% A and 100% B; 10.1-12 min, 98% A and 2% B.</p>Novel tripeptide Gly-Arg-Tyr derived from Ileibacterium valens promotes intramuscular fat deposition via FTO/m6A/YTHDF2-mediated mitotic clonal expansion.Zhejiang UniversityShizhe Xiangblood serummass spectrometry assay<p>A 100 µL aliquot of each serum sample was mixed with 400 µL of 80% methanol. Samples were vortexed, incubated on ice for 5 min, and centrifuged at 15,000 × g for 20 min at 4°C. The supernatant was diluted with mass-spectrometry-grade water to a final methanol concentration of 53%, followed by centrifugation at 15,000 × g for 20 min at 4°C. The resulting supernatant was collected for LC-MS analysis. A pooled quality-control sample was prepared by mixing equal volumes of all experimental samples. A 53% methanol solution was used as the blank.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS15361Shizhe Xiang. Zhejiang University. shizhexiang@zju.edu.cn.Xinxia Wang. Zhejiang University. xinxiawang@zju.edu.cn.<p>Mass spectrometry data were converted to mzXML format and processed using MSnbase, mzR and XCMS. Data processing included peak detection, peak alignment, retention-time correction and peak-area integration. Peak intensities were normalized using the total peak area. Features with excessive missing values or poor reproducibility in quality-control samples were removed before statistical analysis.</p>Treatmentshizhexiang@zju.edu.cn<p>HFD-fed C57BL/6J mice were orally administered live Ileibacterium valens or sterile PBS for 10 weeks. At the end of the experiment, mice were fasted and sacrificed. Whole blood was collected and centrifuged to obtain serum. Serum samples were immediately stored at -80°C until untargeted LC-MS metabolomics analysis.</p>MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicsMus musculusuntargeted metabolomicsIncreased intramuscular fatuntargeted analysisThermo Scientific Q Exactive HF-Xblood serumIleibacterium valensexperimental sampleThermo Scientific Vanquish UHPLC SystemMetabolomicsMus musculusuntargeted metabolomicsIncreased intramuscular fatuntargeted analysisThermo Scientific Q Exactive HF-Xblood serumIleibacterium valensexperimental sample<p>Mass spectrometric analysis was performed using a Thermo Fisher Scientific Q Exactive HF-X mass spectrometer equipped with an electrospray ionization source. Data were acquired separately in positive and negative ion modes over an m/z range of 100-1500. The spray voltage was 3.5 kV, sheath gas flow rate was 35 psi, auxiliary gas flow rate was 10 L/min, capillary temperature was 320°C, S-lens RF level was 60, and auxiliary gas heater temperature was 350°C. Data-dependent MS/MS acquisition was used.</p>5,2',5'-Trihydroxy-3,7,8-trimethoxyflavone 2'-acetateCorticosteronePG(18:2(9Z,12Z)/16:1(9Z))PE-NMe2(16:0/18:1(11Z))Perfluorodecanoic acidCoumestrolGinkgoic acidAltiloxin AL-ErythrulosePolyprenyl phosphateNumersterol AGlucose chloridePC(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:0)Deoxylapachol10-HDoHEPicrasin D2,5-Dihydroxybenzenesulfonic AcidPE(P-16:0/18:2(9Z,12Z))ErucoylacetonePhlomistetraol CPI(20:4(5Z,8Z,11Z,14Z)/18:0)3-ethylphenyl SulfateN-Methylisoleucine1,4-Dihydro-2-methylbenzoicacidcis-7,10,13,16-Docosic acidtraenoic acidKoninginin BPerfluoroheptanoic acidn-Butyl-beta-D-fructopyranosideProstaglandin E23-hydroxy-C10-homoserine lactoneN-Oleoyl-L-Serine2-hydroxyoctanoic acidTOFAPropylenediamine tetra-acetic acidPerfluorooctanoic acidMevalonic acid(2E,6Z)-8-beta-D-glucopyranosyloxy-2,6-dimethyl-2,6-octadienoic acidSecobarbital2-Docosanamidoacetic acid4-MethylcatecholPE(18:0/P-18:0)Metaldehyde6-Methyl-5,6-dihydro-4H-1,3-thiazin-2-amineEbracteatoside C3-(3,4-Dihydroxyphenyl)-1-propanol 3'-glucoside1-palmitoyl-sn-glycerol 3-phosphatecis-2-Dodecenoic acidKainic acidD-alpha-Hydroxyglutaric acid1-StearoylglycerophosphoglycerolLignocerylferulateAllolithocholic acidPristanic acidXanthurenic acidcis-1,2-Dihydro-3-ethylcatecholEpoxycladine C2-Methyl-trans-aconitateHenicosane11,12-DihydrolactaroviolinTectoionol ASuccinyladenosineOleandrosePhosphatidylinositol(34:2)SM(d19:1/16:0)Plakortolide I3a,7a,12a,19-Tetrahydroxy-5b-cholanoic acidL-phenylalanyl-L-histidineBis(3-azidopyridinium)-1,10-decane perchlorate4-Mercaptobutyramidine2-Methyl GraminePlakortolide NCordianol C2-Phenylethyl-2,6-diacetyl beta-D-glucopyranosideKaurenoic acid(1S,2S,4R,8R)-p-Menthane-1,2,8,9-tetrolLysoPE(20:5(5Z,8Z,11Z,14Z,17Z)/0:0)Feigrisolide A(plusmn)12(13)-EpOMEBarogeninFenthion oxonN-Lauroylglycineo-CresolErythramideN-Lauroyl Glutamic acidN(alpha)-Benzyloxycarbonyl-L-leucineFumaric AcidEthyl CaffeateUric acident-16b,19-Kauranediol 19-acetateL-Hexahydro-3-imino-1,2,4-oxadiazepine-3-carboxylic acidN-(1.3-dihydroxyoctadeca-4.14-dien-2-yl)docosanimidic acidCer(d18:1/20:0)Delavayin BDiisopropyl sulfide5-Phenylvaleric acidN-[1,3-dihydroxyoctadec-4-en-2-yl]-2-hydroxytetracosanamideD-Erythrose 4-phosphateCoagulin QLysoPA(i-24:0/0:0)norajmalinePyronine YLysoPE(22:5(7Z,10Z,13Z,16Z,19Z)/0:0)3-Hydroxycapric acidSisomicinAla-LeuMethyl beta-D-Galactopyranoside7-Hydroxy-10-deoxyeucommiolTetradecanedioic acidp-Hydroxyl-ethotoinGlycolithocholic acid 3-sulfate16-Hydroxyhexadecensaure5-DoxylFAHFA 36:11H-Pyrrole-2,5-dione, 1-[4-(2-benzoxazolyl)phenyl]-5-(8,11-Pentadecadienyl)-1,3-benzenediolFAHFA 36:35-(10,13-Nonadecadienyl)-1,3-benzenediolN-Acetyl-L-tyrosineCPA(18:2(9Z,12Z)/0:0)Hexylresorcinol2-[4-hydroxy-3-(sulfooxy)phenyl]acetic acid2-DodecanoneMopidralazineIsolauric acidTerephthalic acidAnobinMaoesin EGluroside(6R,9R)-9-Hydroxy-4-megastigmen-3-oneBestatinPE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/16:0)LysoPI(18:2(9Z,12Z)/0:0)Abietic acid(20R)-17,20-Dihydroxypregn-4-en-3-oneBakers yeast extractFlucytosineSclareolideArg Gly Asp3-(8,11,14-Pentadecatrienyl)phenolEriosematin FTetradecylsulfateSulfotyrosinePterosin N1-Heptadecanoyl-Glycero-3-Phosphate4alpha-Acetyldictyodial3,4-dihydroxybutyrateLysoPE(22:4(7Z,10Z,13Z,16Z)/0:0)PGK1PE(18:1(9Z)/P-16:0)ROCCELLIC ACIDGlutamic acid glutamate16:0 LYSO-PEPE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/P-16:0)N-MethyldihydroquinicinolTaurolithocholic acid12-(2,3-Dihydroxycyclopentyl)-2-dodecanonePetroselinic acid4,6-dinitro-o-cresolBenzonaphthazepineXanthosine(3R)-3,11-dihydroxyundecanoic acidGlcCer(d18:1/16:0)homo-gamma-Linolenic acidAlpha-Muricholic acid3-Methoxy-4-hydroxyphenylethyleneglycol sulfate13E-Labdene-8alpha,15-diol 15-acetate(plusmn)-IbuprofenArtemether2-Benzyl-4-chlorophenolent-AbacavirPerfluorononanoic acidN2,N6-DiacetyllysineCarbazochrome sulfonateGlcCer(d18:1/24:1(15Z))Perfluorobutanoic acid3-(3,4-Dihydroxyphenyl)lactic acid12,14-OctacosanedioneN-Oleoyl GlutamineLepidiumterpenyl esterGlycocholic acid4-Hydroxy-1H-indole-3-acetonitrileGibberoketosterol B2'-DeoxyuridineIndole-3-carboxaldehyde5-Carboxymethyl-2-hydroxymuconate semialdehydeStearic acid17,24-epoxy-25-hydroxy-21-methoxy-3,4-secobaccharanetrans-10-Nonadecenoic acid(C19-1T)Nigrolineaxanthone H2-octanamidoacetic acidAlbifylline3h-Sialic acidPE-NMe2(18:1(9Z)/14:0)11-deoxy-PGE2Methyl palmitoleate10-(Phosphonooxy)decyl methacrylateBisphenol APhe-Ala-GlyLycodineDimethyl mercaptosuccinateAmphorogynine DPyroglutamyl-IsoleucineMonobutyl phthalateTaurochenodeoxycholic acidN-PalmitoyltaurineAntrocinnamomin B(3R)-3,15-dihydroxypentadecanoic acidN-Stearoyltaurine2,3- Dimethoxyphenol sulfateDivinorin FTALAPEROXIDE DGelcoholDocosanoic acidHistidylprolineamideLignoceric acidAllopurinol ribosideHendecanoic acidN4-Acetyl-SulfamethoxazoleNabiloneOleoylglycerone phosphateAlismorientol A5,6-di-Me-PDCxi-3-Hydroxy-5-phenylpentanoic acid O-beta-D-Glucopyranoside(8E,10S,12Z,15Z)-10-Hydroperoxyoctadeca-8,12,15-trienoateBlumenol C glucosideMachaeroceric acid7,15-epoxycaryophyllane-3,5beta-diolIriomoteolide 3aPE(P-18:1(11Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))Dimethylphosphate6-Methoxy-3-(2-thiazolyl)-1H-indole[8]-Paradyl acetateSalicyl alcoholPhenolic steroidPE-NMe2(15:0/22:2(13Z,16Z))HOMOVANILLIC ACID SULFATE16alpha-Hydroxysteroid2,3,5,6-Tetrachloro-4-pyridinolPE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/P-18:0)TaurinePhenol sulfatePerindoprilat9,10-EOT9-(2,3-dihydroxypropoxy)-9-oxononanoic acidPisiferinHeliohoustineTetracosahexaenoic acidPhellodendric acid ASafranalTridecanoylglycine4-nitroquinoline 1-oxideGastrodinTridecanedioic acidGeranyl acetoacetateboheminealpha-Ketoglutaric acidIsostearic acidMyristoleic acid(2E)-4-Hydroxy-3-methylbut-2-en-1-yl trihydrogen diphosphate1-Oxa-2-oxo-3-methylcycloheptane1-NonanethiolGanoderic acid NPentoxifylline3-Epilitsenolide D13-DeazaneplanocinHordatine Bcis-11-Eicosenoic acid7-MethylguanosineHexosyl LPE 18:29(S)-HpODEGlycerophospho-N-Oleoyl Ethanolamine2-Naphthalenesulfonic acidPhaeofuran B(-)-Bornyl acetateMyristic Acid Alkyne12-HHTrE6alpha-malonyloxymanoyl oxide5alpha-CyprinolN-Acetylglutamine4-Vinylphenol sulfateLagopodin A2-Hydroxydocosanoic acid3-tetradecanoyl-sn-glycerolPulicatin DCarbocyclic thromboxane A2N,N'-Diacetylbenzidine6-Thioinosinic acidPE-NMe2(16:0/18:2(9Z,12Z))Curcumol2-(Pentyloxy)ethyl acetateDopamineRosarinIlicifolinoside ANodosinLysoPE(0:0/22:2(13Z,16Z))11'-Carboxy-alpha-chromanolLauryl sulfateGHB-GlucPE(18:1(11Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))Perfluoropentanoic acid5-PentadecylresorcinolSerotonin sulfateIle IleMytilin A2-Hydroxyhexadecanoic acidMycophenolic acidDG(15:0/18:2(9Z,12Z)/0:0)11-Mercaptoundecanoic acidPyridoxal hydrochrolideLysoPE(0:0/20:0)3-(3-Hydroxyphenyl)propionic acid sulfatealpha-[3-(Nitrosoamino)propyl]-3-pyridinemethanolLysoPA(15:0cycw5/0:0)alpha-Caryophyllene alcohol acetate12-hydroxystearic acid4-Nitrobenzoic acid(4S*,6S*)-Dihydroxy-6-(14'Z-nonadecenyl)-2-cyclohexenoneN-Benzoyl-(2R,3S)-3-phenylisoserineOxandroloneNectandrin ASULFAMERAZINEMairetolide EPalmitoyl glucuronideOctyl gallateC18-Sphingosine 1-phosphate; D-erythro-Sphingosine-1-phosphateDolichoic acid-[18-20]Dihydroresveratrol 3-sulfateThyminoseA-O-MethyldihydrobotrydialoneLemnaboursideMurisolinN-Octanoyl-L-homoserine lactone4Z,7Z,10Z,13Z-hexadecatetraenoic acidIndole-3-acetyl-L-valineMulberrofuran LNeodunol(1R,5aS,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylic acid20:2(5Z,9Z)(11Me,15Me,19Me)7-O-AcetylaustroinulinHexosyl LPE 16:0L-MalateStolonic acid BAdipic acid1,2,4,5-TetrahydrotestolactoneLeu-Phe-Asn3-AcetylthiopheneKobusone2-Oxovaleric acid7alpha,12alpha-Dihydroxycholest-4-en-3-one5,9:6,9-Diepoxy-3-megastigmeneSedumoside HN-Hexanoyl-L-homoserine lactone2,8-Dihydroxyquinoline-beta-D-glucuronidePalifosfamide1-Acetoxy-4,6-tetradecadiene-8,10,12-triyneCholesteryl-beta-D-glucosideUndecylenic acidL-Glutamic acidMEDICA 16IsolysergolAgarPE-NMe2(18:0/18:1(9Z))N-Lactoyl-PhenylalanineGlochidionionoside AThreonic acidPE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/18:0)CantharidinHeptadecanoic acidSquamocin KN-Oleoyl-PhenylalanineMelodorinolArabinosylhypoxanthineVanilpyruvic acid4-Fluoro-3-phenoxybenzoic acidAglatomin BPE-NMe2(15:0/20:0)PalmitoylglycinePalmitic acidPE-NMe2(16:0/20:4(5Z,8Z,11Z,14Z))LysoPE(0:0/22:1(13Z))2-hydroxystearate2-HydroxydesogestrelNeoporrigenin BDisodium succinateGlycyl-L-histidineD-Erythronolactonebeta-D-Glucopyranosylurea1,3-Di-O-[2',2'-di-(p-phenylene) isopropylidene] glycerol(-)-VallesamidineNervonic acidCalcitroic acid2-Deoxystreptamine 4-phosphatelysophosphatidic acidEupolauridineSublateriol AHomochlorcyclizineHenryinTricycloalternarene 11aPhytosphingosine-1-PPlatydiolPlatelet-activating factorLysoPE(0:0/22:0)Niduterpenoid BPE(P-18:1(11Z)/22:2(13Z,16Z))LysoPE(0:0/24:6(6Z,9Z,12Z,15Z,18Z,21Z))Quinoline-4-carboxylic acid6alpha-hydroxy-castasterone10,16-Dihydroxyhexadecanoic acid2-PropanoylthiopheneEdaravone6-Hydroxypentadecanedioic acidHydroxyphenyllactic acidNotoginsenoside R105alpha-AndrostaneBisnorbiotinTriacetateImepitoin2'-O-methyl-5-methyluridineIndolylmethylthiohydroximateMonoisodecyl phthalateCoprocholic acid1-Stearoylglycerophosphoserine4R-Hydroxy solifenacinD-Pantothenic acid5.9-tetracosadienoic acidPrekinamycin24-epi-brassinolide5b-DihydrotestosteroneLysoPI(18:1(9Z)/0:0)N-AcetylmuramateLimonenecarboxylic acidOleyl alcoholLysoPE(P-18:1(9Z)/0:0)3,4-Dimethyl-5-propyl-2-furandodecanoic acidH-Val-Val-OH3-DeoxypentosoneAndrost-5-ene groupTerretrione BNitrothal-isopropylFicipyrone ASM(d18:2(4E,14Z)/23:1(9Z))Indoxyl sulfate3-Trifluoromethyl-4-nitrophenolIndole-3-pyruvic acidUndecanedioic acid13-Sulfo-dihydroreynosinPregnan-20-one, 17-(acetyloxy)-3-hydroxy-6-methyl-, (3b,5b,6a)-3,4-Dimethyl-5-pentyl-2-furannonanoic acidAlstonoxine B6alpha-Hydroxy-7-oxo-13,14-dihydrokolavenic acid2,4-DinitrophenylhydrazineHexafluoroisopropanolA Factoroctyl hydrogen sulfateIsovalerylglycineSecosubamolide A3-hydroxylaurateeurysterol B sulfonic acidPassifloricin ADihydrocitrininLancifodilactone Fhexacosanoic acid25(R)-HydroxyprotopanaxadiolHydnocarpic acid2-MethylisoborneolPhenylgalactosideFebrifugineValproic Acid13-Oxo-9,11-octadecadienoic acidLysoPG(18:1(9Z)/0:0)Jioglutin D16,16-dimethyl PGF2betaJioglutin E(3beta,22E,24R)-3-Hydroxyergosta-5,8,22-trien-7-oneHis-ProMeteloidinecis,cis-3-Carboxymuconic acidlysophosphatidylmyoinositolFissoldhimineSulfometuron methylNervonoylacetone11,15,19-trimethyl-5Z-eicosenoic acid28-HomobrassinolideOxyphencycliminebeta-Hydroxymyristic acidCleroindicin Epropyl linoleateVinylacetylglycine2'-O-MethyluridineDi-O-methyldemethoxycurcuminPE(P-18:1(11Z)/20:2(11Z,14Z))(+)-AspidospermidineAncistroquinone F(plusmn)alpha-CMBHCPC(20:3(8Z,11Z,14Z)/16:0)9-deoxy-9-methylene-16,16-dimethyl -PGE22-O-Acetyl-20-hydroxyecdysoneNonane-4,6-dioneSphingosine 1-phosphate (d16:1-P)(6beta,24R)-6-Hydroxystigmast-4-en-3-oneHomalomenol CMorifolineStigmastanediolGalbonolide CIrisoquinHydroxyprolyl-MethionineDehydroabietic acid7a-Epialexaflorine9-(palmitoyloxy)octadecanoic acid3,5-Dibromo-4-hydroxybenzoic acidS-(3-Methyl-2-butenyl) 2-methylpropanethioateValinopineOctadecanedioic acidClorazepate5beta-Cholestane-3alpha,7alpha,12alpha,24-tetrol2-keto palmitic acidPandangolide 1aPterodontoside DHeptane-1-thiolAlpha-N-Phenylacetyl-L-glutamineTrihydroxycoprostanoic acidSarcotin C(2E)-21-hydroxyhenicos-2-enoic acidNORLEUCINESM(d16:1/17:0)20-DihydrodydrogesteroneL-Asparagine(+)-Rauflexine5alpha-Androst-16-en-3-one4-Ethylphenol9,10,13-TriHOMESemiplenamide ESemiplenamide F4-Propylphenol2-Methoxy-10H-phenothiazineStoloniferone TNaringenin triacetate7-Hydroxymethyl-12-methylbenz[a]anthracene sulfateCarbocyclic 3-deazaadenosineAscorbic acid 2-sulfate2-Methyl-4-propyl-1,3-oxathiane4-Methylgallic acid 3-sulfate2,5-Bis(acetylamino)pentanoic acid14-oxo-DoHE(1-)Validamine 7-phosphateStoloniferone GLysoPE(16:1(9Z)/0:0)2-Amino-3-cyclohexylpropanoic acidPE(P-18:0/22:5(4Z,7Z,10Z,13Z,16Z))PE-NMe2(16:0/20:3(5Z,8Z,11Z))3-HYDROXYSUBERIC ACIDSwainsonine N-oxideHamabiwalactone BEudesmic acidMuurolane-4beta,5beta,10beta-triol5,22-dihydroporphyrinAntibiotic WF 3405Conjugated linoleic acids (CLA)N-Acetyltryptamine7-Oxo-8,15-isopimaradien-18-oic acidOleoyltaurineBotryolide D20-hydroxytrioxilin A3(6E,8E,10S,12Z)-10-Hydroxy-3-oxooctadecatrienoic acidIndole-3-acetylglycinealpha-hydroxy Farnesyl Phosphonic AcidOR-1896Caffeic acid 3'-sulfate4-Methoxyphenol sulfateChlorothalonil-4-hydroxy2,4-Di-tert-butylphenolLysoPE(0:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))Androstanediol-17gN-acetylmethionineUridineN-Palmitoyl AlanineLysoPC(20:3(5Z,8Z,11Z)/0:0)(S)-3-[(Cyanophenylmethyl)amino]-3-oxopropanoic acidHuperzine DAlangiobussinine28-HomodolicholideHanamyolSchizonepetoside BSterculic acidNALPHA-ACETYL-L-LYSINEDiacarnoxide BIsoiguesterinTrimetaphosphoric acid4beta,7alpha-dihydroxycholesterolAustralin A8-iso-13,14-dihydro-15-keto-PGF2alphaSorbitan palmitateBotcinolide2,3-Thioepoxy Madolent-16beta-Methoxy-19-kauranoic acidDiisopropyl sulfaten-Hexyl-beta-D-glucopyranosideN-Acetyl-L-glutamic acid(E,E)-Boviquinone 34,6-O-Ethylidene-D-glucoseDibutyl sulfosuccinateCPA(16:0/0:0)5-O-sinapoylquinic acidPE(16:0/20:4(8Z,11Z,14Z,17Z))3-epi-6-Deoxocastasterone10-deoxygerfelinPC(16:0/20:4(5Z,8Z,11Z,14Z))Phenylpyruvic acidPE(P-18:1(11Z)/18:0)PE(22:5(7Z,10Z,13Z,16Z,19Z)/P-18:0)Amotin3-O-(alpha-L-rhamnopyranosyl)-D-ribitolPhe-Trp-Alagamma-Glutamyltyrosine2-(4-Methoxyphenyl)ethyl hydrogen sulfateCLONIXINMethynolide(10S)-Juvenile hormone III acid diolPhenylalanylvalineEthyl N-methyl-1,2,5,6-tetrahydro-pyridine-3-carboxylateTaurocholic acid7-Hydroxy-10-ethoxydehydrodihydrobotrydialLPC 18:1LysoPC(18:1(11Z)/0:0)Butyl methacrylateGlu Ile LeuAmoritin5alpha-Pregnan-3beta,20beta-diol 20-sulfatePE-NMe2(18:0/18:2(9Z,12Z))Ribitol4-Hydroxyestradiol2-Methoxyhexadecanoic acidAspartylphenylalanineGeosminPerfluorohexanoic acidSemilepidinoside ASipholenol FGoniothalamusinSuberic acidbeta-fenchocamphoroneZearalenoneSipholenol Mp-cresol glucuronideBisdemethoxycucurminTenulinBecocalcidiolLevoglucosanPE(18:0/20:4(8Z,11Z,14Z,17Z))4-NitrophenolOctadecanamide15-methyl-15R-PGE2Questiomycin A1-heptadecylglycerone 3-phosphatePC(18:0/18:2(9Z,12Z))LysoPE(0:0/20:1(11Z))3-(Trifluoromethyl)benzoic acid1-heptadecanoyl-glycero-3-phosphoethanolamine2-O-Methylascorbic acidKessyl glycolIsobutylparabenIS_4-BROMO-PHENYLALANINEAspergillide Bcrotonyl-L-carnitineIsethionic acidVal Ile Glu3-Methylsalicylic acid3-(3,4,5-Trimethoxyphenyl)propanoic acidbutyrylglycine3E,5E-tridecadienoic acidStearidonic AcidPiscrocin EPerfluorobutane sulfonic acidLysoPC(20:1(11Z)/0:0)3-exo-hydroxy-1,8-cineoleMogroldelta4-Dafachronic acid11-deoxy-11-methylene-PGD2Cardenolide18:0 LYSO-PELysoPE(20:2(11Z,14Z)/0:0)14,19-DihydroaspidospermatineLysoPC(0:0/16:0)Steviol1-Stearoyl-sn-glycero-3-phosphateBilirubin10,20-Dihydroxyeicosanoic acidCinobufaginSterebin A(E)-4,5-Dihydro-6-(2-(4-pyridinyl)ethenyl)-3(2H)-pyridazinone(-)-MethylenolactocinEkeberin D27-O-MethylpseudomajucinJasmonic acid8-Amino-7-oxopelargonic acidcis-10-Heptadecenoic acidcantharidic acidKankanoside E4,6-Dioxoheptanoic acidHeneicosanoic acid1,7,7-trimethylbicyclo[2.2.1]heptan-2-ol benzoate9-Fluorenylmethyl carbazate4-Pyridylethylmercaptan5-Hydroxyindolin-2-one sulfatePE(P-16:0/20:4(5Z,8Z,11Z,14Z))Musclide BMethyl heptadecanoateMandelic acid sulfatesn-glycero-3-Phosphoethanolamine2-Heptadecanonetetradec-5-ynoic acidDodecanoic acidCoformycinIsoleucyl-Threonine(2E)-19-hydroxynonadec-2-enoic acidClusianonePE(18:2(9Z,12Z)/18:1(11Z))Lanceolatin B (flavonoid)5beta-Cholanic acidDG(a-17:0/12:0/0:0)5-MethoxytryptolineNigerapyrone CN-Acetyl-alpha-D-glucosamine 1-phosphatePyrisoxazolePE(18:1(11Z)/20:4(5Z,8Z,11Z,14Z))PE-NMe2(15:0/22:4(7Z,10Z,13Z,16Z))Diacetoxy-6-gingerdiolTraumatic AcidCreoside I2,3-Dihydro-5-hydroxy-2,2-dipentyl-4,6-di-tert-butylbenzofuranpseudouridinePectinolide CSM(d18:2(4E,14Z)/22:0)13(S)-HODEPectinolide DHyodeoxycholic acidDodecylbenzenesulfonic acidSulfocholic acidPE(18:2(9Z,12Z)/18:0)26-hydroxyhexacosanoic acid7,11,15-Trimethyl-3-methylene-1,2-hexadecanediol diacetate12,14-TriacontanedioneDodecyl glucosideN-Carboxyacetyl-D-phenylalanineBenzetimideLysoPE(0:0/18:0)Allantoin(-)-Hydroxycitric acidLysoPI(20:4(5Z,8Z,11Z,14Z)/0:0)NicotinoylglycineDanshenxinkun B5-HydroxytryptophanRoyal Jelly acidAngelosideNebrosteroid GNebrosteroid F1-palmitoyl-sn--glycero-3-phospho-D-myo-inositolBergeninAlloxydim2-Hydroxy-22-methyltetracosanoic acidCarvacrol methyl etherBriarellin P2-OxosuberateEicosopentanoic acidTetranor-PGF1alphaDidemnidine A5-Bromo-L-tryptophanChloral hydrateArachidic AcidRengyoside BPhomolide A1-Dodecanesulfonic acid, 1-hydroxy-3-oxo-Robustocin2'-O-methylinosineMicromelone Boxalosuccinate(7S)-HydropreneNorsanguinine1-(S)-Methylbutyl-beta-D-glucopyranoside6-Ethylchenodeoxycholic acidEthyl glucuronide12-OPDACitrininAsparaginyl-Gamma-glutamatephosphoethanolamine (P-18:0)Pentadecanoic acidN-Desethyl amodiaquineDibenzothiophene4-Hydroxymethylcatecholdocosyl ferulateN-Palmitoyl Glutamine4-Acetoxy-2-hexyltetrahydrofuranDehydroandrographolidePhenylacetylglycine5-cis-15(R)-Iloprostp-Cresyl sulfateSM(d17:2(4E,8Z)/16:0)11alpha-Hydroxyandrosta-1,4-diene-3,17-dioneCheimonophyllon CN-(3-Oxooctanoyl)-DL-homoserine lactoneDecarboxy-Norlobaric AcidAvermitilolSUBERYL GLYCINEBCA 10CPA(18:0/0:0)1,2,3,4-Tetrahydro-b-carboline-1,3-dicarboxylic acidCPA(18:1(11Z)/0:0)2-Hydroxyphytanic acid9,12,15,18,21-Tetracosapentaenoic acidRadiosuminTauro-alpha-Muricholic acid5-Heptyl-3-methyl-2-furanundecanoic acid8-Epiloganic acidIndolelactic acid11-UndecanolactoneCordobic acid 18-acetateSaussureolideCreoside II4-Hydroxyhygric acid4-Nitrobiphenyl6-Ketomyristic acidcis-7,10,13,16,19-Docosapentaenoic acid3-tert-Butyl-5-methylcatechol2-Hydroxytetracosanoic acidCitric AcidLactapiperanol C(+)-Neothramycin BOogoniol 1Dimethylmalonic acidMethylglucopyranosiduronic acidTetradecanoic acidThymidine 3',5'-cyclic monophosphate2-Methoxytetradecanoic acidMycosporine glutaminolMaoecrystal AStanozolol(plusmn)12-HETEArphamenin B1-Lyso-2-arachidonoyl-phosphatidate(2S,4R)-4-(9H-Pyrido[3,4-b]indol-1-yl)-1,2,4-butanetriol(plusmn)5,6-DHETN-Sulfonyloxy-PhIPAnnuionone B19-Oic-deoxycorticosterone4-HydroxyestronePerindopril Acyl-beta-D-glucuronide(1R,2R,4S,5S,6R,7S)4,5-epoxygermacra-9Z-en-1,2,6-triolPhenylglucuronide3-Aminosalicylic acidLysoPE(18:3(6Z,9Z,12Z)/0:0)LysoPG(18:2(9Z,12Z)/0:0)Moracin OButyl (S)-3-hydroxybutyrate glucosideascaroside C33-AndrostanolLys Trp Lys(+/-)-Ethyl 3-mercapto-2-methylbutanoateAtractyloside G12-NoralliacolidePE(P-16:0/22:5(4Z,7Z,10Z,13Z,16Z))Itoside M2-Hydroxybutyric acidOxaloglutarateLumpidin2-Hydroxyacetophenone sulfate2,2,7,7-Tetramethyl-1,6-dioxaspiro[4.4]non-3-ene3-Methoxytyramine-betaxanthin6-Deoxy-5-ketofructose 1-phosphate2-Heptyl butyrateEnalaprilat2-hydroxy-3-(sulfooxy)benzoic acidDidanosineN-[1.3-dihydroxyoctadec-4-en-2-yl]octadecanamide5-(4-methyl-5-oxo-2H-furan-3-yl)pentanoic acidLumichrome2,6-DinitrotolueneCelerin7,8-Dimethoxyflavanone6-exo-hydroxycineoleTanaparthin alpha-peroxideBesarhanamide APaecilocin DPutaminoxin D9,10-DihydroxystearateCholic acidphosphoethanolamine (P-16:0)Latrunculeic acid2,5-Dimethyl-1,4-dithiane-2,5-diolPC(18:1(11Z)/18:0)1,2-CyclohexanedioneL-3-Phenyllactic Acid2,6-Diamino-9-(2-hydroxyethoxymethyl)purineCyprofuramMenadione bisulfiteAspidospermatineGelsedilamNonanoic acidArbutinalpha-CEHC13-HOTE15-hydroxyculmorinPhthalic acid mono-2-ethylhexyl ester2-Phenyl-3,4-dihydro-2H-thiochromeneHymenoside W(S)-3-Hydroxybutanoic acidL-alpha-Phosphatidylethanolamine5-Hydroxyadamantan-2-oneCertonardosterol A414,15-EE-5(Z)-ERhodomollein XIVCis-9,10-methylenehexadecanoic acidLysoPE(20:3(11Z,14Z,17Z)/0:0)Hirsutenol E3-O-methyl-alpha-L-rhamnoseDodecanedioic acidChaksineCrocusatin EDimethachlor-ESA4-MethyldibenzothiopheneFluoresceinLipoamideLysoPG(16:0/0:0)N-Acetyl-L-alanineIrisresorcinolFiciolide GN-Decanoylglycine(4S)-4-Hydroxy-5-phosphonooxypentane-2,3-dione8phiC8SPC13-Hydroxydocosanoic acid2-Keto-L-gulonate3,3'-DiindolylmethaneThymidineAlbocycline M-1(3R)-3,9-dihydroxynonanoic acid3-Hydroxysebacic acidLimazepine APantetheineTiglylglycineLimazepine Ccis-10-Pentadecenoic acidCrocusatin GCarthamosterone B(4-tert-Butyl-phenoxy)-acetic acid1-CarboxyethyltyrosineIndole-3-acetaldehyde3-hydroxytridecanoic acidDidesethyl ChloroquineN-acetylphenylalaninelysine phosphoester2,2'-(3-methylcyclohexane-1,1-diyl)diacetic acidLauroyl peroxidealpha,alpha-DimethylanisalacetoneFAHFA(18:1(9Z)/13-O-18:0)Isocitratecyclic 6-HydroxymelatoninPE(20:4(8Z,11Z,14Z,17Z)/P-18:0)Ieodomycin BTimosaponin A1Indoxyl glucuronide2-MethylcitrateOPC-4:03-propylbenzene-1,2-diol3-HepteneoylglycineTetrahydro-2-methyl-3-furanthiolTriclosan sulfate1H-Imidazole, 2-(2-benzofuranyl)-4,5-dihydro-Ethyl(E,Z)-decadienoateEstetrolTBHQ6-Acetoxy-7-hydroxymyrcene-7-O-beta-D-glucopyranoside-2'-O-acetateAzelaic acidPartheninPhenetoleProstaglandin F2a11-deoxy-16,16-dimethyl-PGE22-GonenCIS-3-HEXENYL METHYL CARBONATEN-(2-Guanidinoethyl)-5-isoquinolinesulfonamideDeoxycholic acid10-Hydroxydecanoate2-Hydroxymyristic AcidGaliridosideDiphenylphosphateSuccimerfalseUntargeted LC-MS metabolomics profiling of mouse serum following I.v treatment compared with PBS controlsThis study investigated serum metabolic alterations associated with I.v treatment in mice. Serum samples from I.v-treated mice and PBS-treated controls were analyzed by untargeted LC-MS metabolomics in both positive and negative ionization modes. The deposited dataset contains mzXML spectral data from four biological replicates per group and supports comparative analysis of treatment-associated metabolic profiles.2026-09-302026-08-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