Identification and integration was based on retention times from authenticated standards.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - hilic"],"chromatography_protocol":["Cell extracts were analyzed by ultra-high-performance liquid chromatography (UHPLC) coupled to a Q Exactive Plus mass spectrometer (UHPLC-MS) with electrospray ionization and randomized sample injection. LC separation used an XBridge BEH Amide XP column and a gradient of solvent A (aqueous, pH 9.4) and solvent B (acetonitrile). Two flow paths were operated in tandem, staggered by 20 minutes. The 5 μl injection was run at 150 μl min−1.
"],"publication":["Metabolite assay of T cells that are antigen-specially activated by viscoelastic synthetic antigen presenting cells."],"submitter_affiliation":["University of California Los Angeles"],"submitter_name":["Youcheng Yang"],"organism_part":["T cell"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["After washing, metabolite extracts were collected, centrifuged at 17,000g, dried under nitrogen gas, and reconstituted in HPLC-grade water (10–15 μl per 106 cells).
"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15444"],"author":["Youcheng Yang. University of California Los Angeles. youcheng@g.ucla.edu.","Li Song. University of California, Los Angeles. songli@ucla.edu."],"data_transformation_protocol":["Data was collected with Xcalibur and analyzed using MAVEN
"],"study_factor":["SynVAC viscoelasticity"],"submitter_email":["youcheng@g.ucla.edu"],"sample_collection_protocol":["2 × 106 ESO-T cells activated for 2 days were vacuum filtered onto nylon membranes (0.45 μm). Each filter was submerged, cell-side down, in 400 μl of precooled extraction solvent (40:40:20 acetonitrile/methanol/water) at −20 °C for 20 minutes.
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","normal","targeted analysis","T cell","solvent blank","Homo sapiens","University of California, Los Angeles","Thermo Scientific Q Exactive Plus","Thermo Scientific Accela UHPLC System"],"curator_keywords":["Metabolomics","normal","targeted analysis","T cell","solvent blank","Homo sapiens","University of California, Los Angeles","Thermo Scientific Q Exactive Plus","Thermo Scientific Accela UHPLC System"],"mass_spectrometry_protocol":["The mass spectrometer operated in negative- and positive-ion modes with a resolution of 140,000 and a scan range of m/z 60–2,000.
"],"additional_accession":[]},"is_claimable":false,"name":"Metabolite assay of T cells that are antigen-specially activated by viscoelastic synthetic antigen presenting cells","description":"Adoptive T cell receptor (TCR)–based immunotherapy offers a powerful strategy to target solid tumors. However, the physical properties of the antigen-presenting interface regulate T cell metabolic programming remains poorly defined. Here we develop synthetic viscoelastic activating cells (SynVAC), artificial antigen-presenting cells whose stress-relaxation kinetics are chemically tuned independently of stiffness and of peptide–MHC (pMHC) and co-stimulatory ligand density. SynVAC-pMHC provides a defined, scalable platform that outperforms monocyte-derived dendritic cells to enrich antigen-specific CD8+ T cells. Fast-relaxing SynVAC-pMHC amplifies TCR signaling and mTOR activity, drives metabolic remodeling, and programs antigen-specific T cells toward an energetically favorable state with improved memory-like features after expansion. TCR-T cells expanded using fast-relaxing SynVAC-pMHC exhibit superior persistence and durable antitumor activity in melanoma and ovarian cancer models. These results establish cell-mimetic viscoelasticity as a key biophysical cue governing T cell metabolism and differentiation and offer a fully synthetic strategy for manufacturing potent TCR-T cells.
","dates":{"publication":"2026-09-03","submission":"2026-08-24"},"accession":"MTBLS15444","cross_references":{}}