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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/m_MTBLS15444_LC-MS_alternating_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/a_MTBLS15444_LC-MS_alternating_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/s_MTBLS15444.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/SRB_C.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/MRB_C.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/MRB_A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/FRB_A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/IL2_B.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/FRB_C.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/Quie_C.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/Blank.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/SRB_B.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/Quie_A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/MRB_B.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/IL2_C.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/IL2_A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/Quie_B.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/FRB_B.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444/FILES/RAW_FILES/SRB_A.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15444Identification and integration was based on retention times from authenticated standards.MetaboLightsPublicLiquid Chromatography MS - alternating - hilicCell extracts were analyzed by ultra-high-performance liquid chromatography (UHPLC) coupled to a Q Exactive Plus mass spectrometer (UHPLC-MS) with electrospray ionization and randomized sample injection. LC separation used an XBridge BEH Amide XP column and a gradient of solvent A (aqueous, pH 9.4) and solvent B (acetonitrile). Two flow paths were operated in tandem, staggered by 20 minutes. The 5 μl injection was run at 150 μl min−1. Metabolite assay of T cells that are antigen-specially activated by viscoelastic synthetic antigen presenting cells.University of California Los AngelesYoucheng YangT cellmass spectrometry assayAfter washing, metabolite extracts were collected, centrifuged at 17,000g, dried under nitrogen gas, and reconstituted in HPLC-grade water (10–15 μl per 106 cells).Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS15444Youcheng Yang. University of California Los Angeles. youcheng@g.ucla.edu.Li Song. University of California, Los Angeles. songli@ucla.edu.Data was collected with Xcalibur and analyzed using MAVENSynVAC viscoelasticityyoucheng@g.ucla.edu2 × 106 ESO-T cells activated for 2 days were vacuum filtered onto nylon membranes (0.45 μm). Each filter was submerged, cell-side down, in 400 μl of precooled extraction solvent (40:40:20 acetonitrile/methanol/water) at −20 °C for 20 minutes. MetabolomicsMetabolomicsnormaltargeted analysisT cellsolvent blankHomo sapiensUniversity of California, Los AngelesThermo Scientific Q Exactive PlusThermo Scientific Accela UHPLC SystemMetabolomicsnormaltargeted analysisT cellsolvent blankHomo sapiensUniversity of California, Los AngelesThermo Scientific Q Exactive PlusThermo Scientific Accela UHPLC SystemThe mass spectrometer operated in negative- and positive-ion modes with a resolution of 140,000 and a scan range of m/z 60–2,000. falseMetabolite assay of T cells that are antigen-specially activated by viscoelastic synthetic antigen presenting cellsAdoptive T cell receptor (TCR)–based immunotherapy offers a powerful strategy to target solid tumors. However, the physical properties of the antigen-presenting interface regulate T cell metabolic programming remains poorly defined. Here we develop synthetic viscoelastic activating cells (SynVAC), artificial antigen-presenting cells whose stress-relaxation kinetics are chemically tuned independently of stiffness and of peptide–MHC (pMHC) and co-stimulatory ligand density. SynVAC-pMHC provides a defined, scalable platform that outperforms monocyte-derived dendritic cells to enrich antigen-specific CD8+ T cells. Fast-relaxing SynVAC-pMHC amplifies TCR signaling and mTOR activity, drives metabolic remodeling, and programs antigen-specific T cells toward an energetically favorable state with improved memory-like features after expansion. TCR-T cells expanded using fast-relaxing SynVAC-pMHC exhibit superior persistence and durable antitumor activity in melanoma and ovarian cancer models. These results establish cell-mimetic viscoelasticity as a key biophysical cue governing T cell metabolism and differentiation and offer a fully synthetic strategy for manufacturing potent TCR-T cells.2026-09-032026-08-24MTBLS15444