MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/m_MTBLS15452_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/m_MTBLS15452_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/s_MTBLS15452.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/a_MTBLS15452_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/a_MTBLS15452_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/Sham_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/Sham_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/NR_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/NR_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/Sham_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/NR_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/Sham_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/Sham_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/NR_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/NR_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/NR_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/NR_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/Sham_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/NR_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/Sham_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/NR_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/Sham_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/NR_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/Sham_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/Sham_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/NR_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/NR_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/neg/Sham_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452/FILES/RAW_FILES/pos/Sham_1.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15452Metabolites were annotated by matching accurate molecular weights and MS/MS fragment spectra against HMDB, Massbank, LipidMaps, mzCloud, KEGG and in-house metabolite databases. A total of 424 metabolites were identified in this study.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseSamples were separated by ultra-high-performance liquid chromatography at column temperature of 40 degrees C. Gradient elution was performed in positive and negative ion modes, with an injection volume of 2 micro L. The auto-sampler was kept under low-temperature condition.The dynamic changes and mechanism of no reflow injury after myocardial ischemia-reperfusion.Peking UniversityDuo KeaiHeart tissuemass spectrometry assayAppropriate amounts of animal tissue samples were transferred into 2 mL centrifuge tubes. Metabolites were extracted using methanol-chloroform-water extraction solution, followed by grinding, room-temperature sonication and ice-bath incubation. After centrifugation, the supernatants were collected, concentrated to dryness, reconstituted with solvent containing internal standard 2-chloro-L-phenylalanine, and filtered with 0.22 micro m membrane for subsequent LC-MS analysis.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS15452ting chen. Hunan University of Chinese Medicine. chenting520@hnucm.edu.cn.ting chen. Hunan University of Chinese Medicine. 2956566536@qq.com.Raw mass spectrometry files were converted to mzXML format using MSConvert in Proteowizard software. Peak detection, filtering and alignment were accomplished with the R XCMS package. Batch effects were corrected based on QC samples, and features with RSD > 30 % were removed.Groupkeaiduoduo998@126.comAnimal tissue samples were stored at low temperature after collection. Metabolites were extracted with methanol-chloroform-water system, and 2-chloro-L-phenylalanine was added as internal standard. After grinding, sonication and centrifugation, the supernatants were concentrated, reconstituted and filtered through 0.22 micro m membrane. QC quality-control samples were prepared by mixing equal aliquots of supernatants from all test samples.MetabolomicsMetabolomicsMus musculusHeart tissueNo reflow after myocardial ischemia-reperfusionTissue sampleuntargeted analysisTranscriptomics and MetabolomicsTCA cycleThermo UHPLC systemThermo Orbitrap mass spectrometerdynamic changesMetabolomicsMus musculusHeart tissueNo reflow after myocardial ischemia-reperfusionTissue sampleuntargeted analysisTranscriptomics and MetabolomicsTCA cycleThermo UHPLC systemThermo Orbitrap mass spectrometerdynamic changesHigh-resolution Orbitrap mass spectrometer equipped with ESI ion source was adopted. Both full-scan MS and MS/MS fragment information were acquired, and detections were carried out under positive- and negative-ion modes respectively.M599T505_1M278T304M449T350M617T503M464T540M580T290_1M481T345M580T290_2M279T590M826T239_2M466T159M449T354M826T239_1M390T500M362T293M460T465_3M278T316M716T435_1M442T473M331T175M593T542_3M593T542_2M287T335M135T567M265T279M131T67M191T234M907T259_1M296T55_2M191T236M925T545_4M233T63M907T259_2M583T441_1M117T507M730T434_2M245T88M730T434_1M449T379M396T40M233T56M749T38_1M749T38_2M345T469M239T467M289T60_1M289T60_2M608T493_2M608T493_1M990T468_1M793T45_1M530T458M928T540_1M641T621_1M665T441M256T234M997T243M269T47M233T49M233T40M628T145M686T655_1M437T61_3M459T629_1M434T687M200T225M281T658_2M269T302M588T53M274T296M608T502M441T239M771T453_2M569T650M776T471_3M397T418M256T298M135T522M786T358_2M786T358_1M404T41_2M198T159M482T582M404T41_1M680T41_1M680T41_2M342T619M388T482_2M376T559M597T437_3M182T257M793T558_2M767T43_2M339T278M767T43_1M335T53_1M311T548M384T56M269T324M780T601_1M451T465M359T95M754T631M594T233M265T224M425T623M449T50M449T54M576T59M240T384M922T470_3M922T470_2M372T60M159T237M449T342M723T234_2M397T433M518T304_1M843T593_1M608T480_1M608T480_2M180T641M477T641M696T534_2M696T534_1M527T514_3M323T41M595T240_2M333T307_1M950T256_2M973T542_3M601T287_2M301T274M998T236_1M376T509M301T279M901T44_1M810T469_2M901T44_2M431T646_1M757T263_1M270T243M638T41M570T516M431T646_3M475T535_1M475T535_2M673T273M975T521_2M323T61M341T404M241T519M347T28M512T461_1M512T461_2M815T251_5M854T269_2M301T254M854T269_1M661T627_2M358T572M653T482_2M786T135M301T268M528T310M534T537M689T423_2M561T504M956T489_1M956T489_2M666T288_1M534T402_1M666T288_2M221T57M531T456_2M531T456_3M858T575M507T435M233T26M974T522_2M974T522_1M678T444_3M581T640_3M678T444_4M686T258M599T543M462T65M284T590M199T358M534T528M335T59M409T268M638T39_2M341T443M191T267M398T626M948T234_1M553T240_1M199T325M398T620M810T469_1M694T435_2M694T435_4M694T435_3M200T277M292T287M918T524_2M826T627_1M765T231M254T602M216T182M191T253M183T424M776T231_3M993T223M363T452M895T245_1M895T245_2M981T499_1M678T590M400T597_2M562T522_1M388T367M158T93M598T275M227T118_2M205T41M562T522_2M229T271M968T538_1M575T643_1M968T538_2M716T66_1M971T581_1M955T274_1M971T581_3M955T274_2M656T455_2M656T455_1M716T66_2M716T66_3M450T422_2M271T245_2M178T449M406T368M537T526_2M537T526_1M430T594M475T361_2M497T436_2M497T436_1M599T313_1M332T323M229T256M341T489M542T231M299T229M270T298M419T536_2M575T567_2M797T401_1M306T606M459T535M725T449_1M309T456M195T269M630T236_1M297T640M432T364_2M229T262M412T39_1M222T258M374T459_2M457T535_1M457T535_2M301T308M357T260M692T474_2M692T474_3M692T474_1M229T55M269T51_1M158T52M155T273M371T332M283T248M115T119M433T459_1M309T436M701T646_1M839T239M222T238M534T494_2M634T612_3M516T522M325T54_3M533T327M498T465_2M277T129M749T422_2M178T4M909T256_3M608T551_2M350T479M909T256_2M909T256_1M388T421_2M894T215_1M476T489_4M876T505M529T625_1M294T94M146T48M476T489_3M787T459_3M484T254M523T497_2M261T129M515T42M719T439_4M534T584M379T408M630T654_1M745T577M204T302M283T258M146T50M217T51M552T56M628T44_1M688T473_1M375T387M122T58M422T498_2M666T530_1M688T473_2M480T649_1M539T476_3M356T255_1M350T427M552T50M504T553M238T233M552T52M552T47M375T396M574T533M660T650_2M660T650_3M240T280M446T548M605T455_1M182T294M770T454_2M626T497M552T44M247T161M494T426M182T259M769T444_1M351T52M351T51M522T619M309T63_1M363T39M604T442_2M917T609_2M826T238M350T407M958T526_3M826T240M247T191M647T467_2M555T467_1M668T458_3M958T526_1M668T458_2M556T543_1M564T37M460T585M806T576M788T536M806T577M223T129_1M661T650_1M223T129_2M522T620M280T431M182T270M279T616M826T249M725T220M708T343_2M180T685M523T199_2M339T4M486T233_2M293T412M802T484_2M231T320M789T592_2M796T505_2M522T460_3M735T274_2M604T567M244T307M789T592_1M446T89M774T43_3M302T554M611T478_3M611T478_2M101T508M269T67M432T144M231T333M806T67_2M508T514_1M217T323M508T514_2M294T47M833T579M530T507M351T652M657T479_2M282T67M568T436M282T65M239T425M626T487M984T273M419T255_1M552T433M387T43M389T588M626T481M530T528_1M624T537_1M109T50M774T43_2M446T68M706T454_2M375T374M367T436M282T59M796T505_1M566T537_2M655T270M347T129M411T266M919T524M560T367M296T498M632T604_1M632T604_2M615T454_2M369T274M416T518M661T452_1M586T241_2M507T361M586T379M314T258M586T241_1M727T577_1M359T663M610T510M423T122_1M538T467M360T566M345T357M427T158M327T521M295T243M278T94M291T99M675T590_1M675T590_2M538T498M240T233M338T5M449T499M269T401M393T255M769T426M398T548M930T538_1M295T253M568T516M597T658_2M305T233M453T53_2M314T275M398T544M735T44_1M188T94M416T528M729T558_2M419T384M375T459M322T242M322T241M457T255M954T274_1M740T667_2M660T645_1M271T359M607T478_2M723T475_1M434T560M652T98_2M358T424M291T49M467T274M451T328_2M560T415M672T429_2M427T121M672T429_1M450T312M383T218M314T298M504T526M472T234M304T51M553T633M278T57M323T498M605T233_2M358T439M629T274M287T254M425T513M482T459M625T644_2M510T590_1M799T37_1M416T543M537T67_3M568T610_1M291T53M531T41M291T50M656T449_1M488T357M256T383M656T449_2M327T327_2M239T384M663T475_1M663T475_4M248T343M556T495M550T545_1M567T253_4M490T495M731T248M587T468_2M634T444M504T508M232T423M526T476_1M451T597M737T425_2M473T499M553T392_1M404T55M477T54M766T558_2M766T558_1M177T155M367T47_1M842T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dynamic changes and mechanism of no reflow injury after myocardial ischemia-reperfusionThis study aims to investigate the dynamic changes and correlation of no-reflow (NR) injury following myocardial ischemia and reperfusion, and to explore the underlying mechanism of NR injury through transcriptomic and metabolomic analysis. Methods: Healthy male SD rats were divided into two batches: fixed ischemia time and fixed reperfusion time. In the model group, the left anterior descending coronary artery was ligated and subsequently reperfused, while the Sham group, underwent threading without ligation. The myocardial area with NR and the ischemia area were assessed using thioflavin S and Evans blue staining, the cardiac function was measured by echocardiography, the myocardial enzyme activity was detected by automatic biochemical analyzer, and the mechanisms of NR phenomenon were analyzed via transcriptomic and metabolomic approaches, with validation through Western blot (WB) analysis. Results: The model group showed obvious NR phenomenon. During fixed reperfusion, NR and ischemia were the largest at 4 h of ischemia. In fixed ischemia, NR and ischemia area were the largest when reperfusion was performed for 24 hours. Compared with the Sham group, the myocardial enzyme activity of the rats in the vast majority of the model group was increased, and the cardiac function of the rats in all model groups was impaired. Metabolomics results showed that the main differential metabolites were citric acid and succinic acid, etc when NR occurred, and the main pathways there oxidative phosphorylation and TCA cycle. Transcriptome analysis showed that the expression levels of branched-chain aminotransferase 1 (Bcat1), 4-hydroxyphenylpyruvate dioxygenase (HPD) and glutathione transferase Zeta 1 (GSTZ1) were changed when NR occurred. The results of the enrichment of transcriptome biological functions involve tyrosine metabolism, etc., and are related to amino acid metabolites related to the TCA cycle. Metabolomics and transcriptomics results were analyzed using MetaboAnalyst 6.0 to show that when NR occurs, it affects energy metabolism and causes NR damage by regulating the TCA cycle, which is mainly related to the metabolism of amino acids such as tyrosine. WB results showed that the protein expression of Bcat1, HPD and GSTZ1 decreased after NR injury.2026-08-252026-08-25MTBLS15452