MetaboLights

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features detected by untargeted LC-MS analysis were annotated based on accurate mass, retention time, and, where available, tandem mass spectrometry fragmentation information. MS/MS spectra acquired from pooled quality-control samples were used to support metabolite annotation and identification.MetaboLightsPublicLiquid Chromatography MS - positive - reversed-phase-chromatographyLiquid Chromatography MS - negative - reversed-phase-chromatographyChromatographic separation was performed using a Thermo Scientific Vanquish UHPLC system equipped with a Hypersil GOLD aQ column (100 × 2.1 mm, 1.9 μm). The column temperature was maintained at 40 °C and the flow rate was 0.2 mL/min. The mobile phases consisted of 0.1% formic acid in water (A) and methanol (B), using reversed-phase chromatography.Time-resolved quantitative microbiome profiling and metabolomics reveal domain-specific responses during Burkholderia strain B23 colonization in the citrus rhizosphere.Yunzeng ZhangYangzhou Universitysolventrhizospheremass spectrometry assayMetabolites were extracted from citrus rhizosphere soil samples for untargeted LC-MS metabolomic analysis. Extracted samples were prepared for chromatographic analysis, and pooled quality-control samples and solvent blanks were included to monitor analytical reproducibility and background signals.Citrusblank samplehttps://www.ebi.ac.uk/metabolights/MTBLS15457Yunzeng Zhang. Yangzhou University. yzzhang@yzu.edu.cn.Jinyan Yu.LC-MS spectral data acquired in positive and negative ionization modes were processed separately and converted to mzXML format for downstream data processing and repository submission. MS/MS spectra acquired from pooled quality-control samples were exported in MGF format for metabolite annotation.Time after inoculationyzzhang@yzu.edu.cnRhizosphere soil samples were collected from citrus plants immediately before B23 inoculation (0 dpi) and at 1, 3, and 9 days post inoculation (dpi). Five biological replicates were collected at each sampling time point and used for untargeted metabolomic analysis.MetabolomicsCitrusThermo Scientific Vanquish UHPLC SystemMetabolomicsblank sampleuntargeted analysisAgricultural Inoculantssolventexperimental blankQ Exactive HFmzML formatrhizosphereuntargeted metabolite profilingThermo Scientific Vanquish UHPLC SystemCitrusMetabolomicsblank sampleuntargeted analysisAgricultural Inoculantssolventexperimental blankQ Exactive HFmzML formatrhizosphereuntargeted metabolite profilingMass spectrometric analysis was performed using a Thermo Scientific Q Exactive HF mass spectrometer coupled to the UHPLC system. Samples were analyzed separately in positive and negative ionization modes over an m/z range of 100–1500. Data-dependent tandem mass spectrometry was additionally performed on pooled quality-control samples to obtain MS/MS spectra for metabolite annotation.falseUntargeted metabolomics reveals temporal rhizosphere metabolic responses during colonization of citrus by Burkholderia strain B23This study investigated temporal changes in the citrus rhizosphere metabolome during colonization by the beneficial bacterium Burkholderia vietnamiensis B23. Rhizosphere soil samples were collected from uninoculated plants and at 1, 3, and 9 days post inoculation (dpi) with B23, representing the initial, active, and relatively stable stages of rhizosphere colonization. Untargeted metabolomics was performed to characterize changes in rhizosphere metabolites associated with B23 establishment. The dataset was generated to determine how the rhizosphere metabolic environment responds over time to the introduction of a beneficial bacterial strain and to support integrated analyses of microbial colonization, resident rhizosphere microbiome dynamics, and metabolite changes. The study provides a time-resolved metabolomic dataset for investigating the ecological processes associated with the establishment of an introduced beneficial bacterium in the plant rhizosphere.2026-08-252026-08-25MTBLS15457