MetaboLights

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features were annotated using MS-DIAL based on accurate mass and MS/MS spectral information. MS/MS spectra were matched against publicly available reference spectral databases, including GNPS, MassBank, MassBank Europe, CASMI, and other MS/MS spectral libraries available in MS-DIAL. Metabolite annotations were assigned based on the matching results of the detected features with reference spectra.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseChromatographic separation was performed using an Agilent 1290 Infinity UHPLC system equipped with an Agilent Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm). The column temperature was maintained at 40°C and the flow rate was 0.3 mL/min. The mobile phases consisted of 0.1% formic acid in water (A) and acetonitrile (B).Hepatic untargeted metabolomic profiling associated with the immunoprotective effects of banana flower polysaccharides.Ting ChenState Key Laboratory of Food Science and Resources, Nanchang Universitylivermass spectrometry assayLiver tissue (40 mg) was homogenized with 200 μL of ultrapure water and 800 μL of methanol. The samples were vortexed for 2 min and incubated overnight at 4°C. The samples were then centrifuged at 14,000 rpm for 15 min. The resulting supernatants were filtered through a 0.22 μm organic membrane and stored at −80°C until LC-MS analysis. Quality control (QC) samples were prepared as pooled aliquots of the study samples and analyzed together with the biological samples.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS15462Ting Chen. Nanchang University. 3295642117@qq.com.Raw LC-MS data files in Agilent .d format were processed using MS-DIAL to perform peak detection, peak alignment, and feature extraction, generating a metabolite abundance table. The resulting abundance table was subsequently imported into SIMCA for multivariate statistical analysis, including OPLS-DA, calculation of variable importance in projection (VIP) values, and permutation testing. Differential metabolites were screened based on a VIP value > 1 and a P value < 0.05.Experimental group3295642117@qq.comLiver tissues were collected from mice after completion of the corresponding experimental treatments. Approximately 40 mg of liver tissue from each mouse was collected for metabolomics analysis. Samples were immediately processed for metabolite extraction or stored at −80°C until metabolite extraction and LC-MS analysis.MetabolomicsGut microbiotabiological sampleMetabolomicsMus musculusliveruntargeted analysisreverse-phase LCHost metabolic remodelingpositiveAgilent 6545 Q-TOFAgilent 1290 Infinity UHPLCAgilent 1290 Infinity UHPLC systemImmune homeostasisnegativeBanana flower polysaccharidespooled QCelectrospray ionization (ESI)Agilent Eclipse Plus C18Agilent 6545 Quadrupole Time-of-Flight (Q-TOF) mass spectrometerGut microbiotabiological sampleMetabolomicsMus musculusliveruntargeted analysisreverse-phase LCHost metabolic remodelingpositiveAgilent 6545 Q-TOFAgilent 1290 Infinity UHPLCAgilent 1290 Infinity UHPLC systemImmune homeostasisnegativeBanana flower polysaccharidespooled QCelectrospray ionization (ESI)Agilent Eclipse Plus C18Agilent 6545 Quadrupole Time-of-Flight (Q-TOF) mass spectrometerMass spectrometric analysis was performed using an Agilent 6545 Quadrupole Time-of-Flight (Q-TOF) mass spectrometer equipped with an electrospray ionization (ESI) source. Data were acquired over an m/z range of 100–1000 in both positive and negative ionization modes.low score: Docosatetraenoic acidlow score: PFCA-H; C22H2F42O2low score: LTB4-[d4]low score: PFCA-H; C15H2F28O2low score: sclareollow score: 3'-Dephosphocoenzyme Alow score: Glutathionelow score: MMV688372low score: PFCA-H; C13H2F24O2low score: Sphingomyelin d18:1-C17:0low score: Isotectorigenin, 7-Methyl Etherlow score: Phosphatidylcholine 18:0-20:4low score: Xanthinelow score: 11.14.17-eicosatrienoic acidlow score: PI 36:4low score: PE 44:10low score: Phosphatidylethanolamine lyso 20:0low score: Palmitic acidlow score: Stearic acidlow score: Hydroxyprogesteronelow score: maltotrioselow score: LPC 16:0low score: (2-aminoethoxy)[2-hydroxy-3-(octadecanoyloxy)propoxy]phosphinic acidlow score: Phosphatidylcholine 14low score: Methylxanthoxylinlow score: 16:0 PS (1,2-dihexadecanoyl-sn-glycero-3-phospho-L-serine (sodium salt))low score: Taurinelow score: PS 42:10low score: D-Glucose-6-phosphatelow score: Phosphatidylcholine lyso 17:0low score: Dihydroxy (3Alpha,12Alpha)Pregnan-20-Onelow score: Guanosine 5'-diphosphate-D-mannoselow score: HEPTADECANOATElow score: Flavin adenine dinucleotidelow score: Germinalinelow score: CDP-ETHANOLAMINElow score: LTD4-[d5]low score: Chlordiazepoxidelow score: Glutathione (oxidized)low score: Kaempferol glucuronidelow score: NCGC00380958-01!2-[[2-[2-[2-[2-[[2-[2-(2-hydroxypropanoylamino)-3-methylbutanoyl]oxy-3-methylbutanoyl]amino]-3-methylbutanoyl]oxypropanoylamino]-3-methylbutanoyl]oxy-3-methylbutanoyl]amino]-3-methylbutanoic acidlow score: Glucocheirolinlow score: (Methylsulfinyl)hexyl glucosinolatelow score: LPE 16:0low score: 2-(hydroxymethyl)-6-(6-hydroxy-6-methyl-3-propan-2-ylcyclohex-3-en-1-yl)oxyoxane-3,4,5-triollow score: PFCA-H; C17H2F32O2low score: Phosphatidylinositol lyso 18:1low score: URIDINE 5'-DIPHOSPHOGALACTOSElow score: 4-hydroxy-5-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-1H-benzo[f][2]benzofuran-3-onelow score: Phosphatidylcholine 17:0-18:2low score: PC 40:9low score: 6-Phosphogluconic acid Barium salt hydratelow score: PFCA-H; C21H2F40O2low score: Argopsinlow score: PFCA-H; C14H2F26O2low score: 3-(3,4-dihydroxyphenyl)-5,7-dihydroxy-6,8-bis(3-methylbut-2-enyl)chromen-4-onelow score: PFCA-H; C12H2F22O2low score: Arachidonic acidlow score: Oleic acidlow score: 5-Hydroxymethylcytidinelow score: Ortophosphatelow score: FUSIDIC ACIDlow score: FLAVIN ADENINE DINUCLEOTIDElow score: [2-hydroxy-3-[hydroxy-[2,3,4,5,6-pentahydroxycyclohexyl]oxyphosphoryl]oxypropyl] octadecanoatelow score: D-Fructose-6-phosphate disodium salt hydratelow score: (E)-Hexadec-9-enoic acidlow score: octadecanoic acidlow score: PI 38:4low score: NCGC00380367-01_C21H24O5_7-Benzofurancarboxylic acid, 2,3-dihydro-2-(1-hydroxy-1-methylethyl)-4-methoxy-6-(2-phenylethyl)-low score: Erioderminlow score: Phosphatidylcholine alkenyl 16low score: PFCA-H; C16H2F30O2low score: LPC 18:1low score: 5-(5-methoxycarbonyl-5,8a-dimethyl-2-methylidene-3,4,4a,6,7,8-hexahydro-1H-naphthalen-1-yl)-3-methylpentanoic acidlow score: (S)-LACTATElow score: Taurocholic acidlow score: PFCA-unsaturated; C21HF39O2low score: Butylparabenlow score: NCGC00381195-01!(2E,6E,12E)-19-(2-amino-2-oxoethyl)-9,11-dihydroxy-8-methoxy-10,12,14-trimethyl-15-oxohenicosa-2,6,12-trienedioic acidlow score: Phosphatidylinositol lyso 20:4low score: URIDINE 5'-DIPHOSPHO-N-ACETYLGALACTOSAMINElow score: PFCA-H; C20H2F38O2low score: neobavaisoflavonelow score: Palmitic Acidlow score: 10-Nonadecenoic acid (19:1(n-9))low score: GLUTAMINElow score: Phosphoric acidlow score: (2S,3S)-3,5,7-trihydroxy-6-methyl-2-(3,4,5-trihydroxyphenyl)-2,3-dihydrochromen-4-onelow score: Phosphatidylinositol lyso 16:0low score: PFCA-H; C19H2F36O2low score: Sulfobacin Blow score: LPE 18:1low score: Harmanlow score: Linoleic acidlow score: Phosphatidylethanolamine lyso 18:0low score: 9-HODElow score: Gly-Glylow score: (2E,4E)-1-[(2R,6S,14S,22S,25R)-25-(3,3-dimethyloxiran-2-yl)-15-methyl-1,3,13,15-tetraazaheptacyclo[18.4.1.0?,?.0?,??.0?,??.0??,??.0??,??]pentacosa-7,9,11,16(21),17,19-hexaen-3-yl]hexa-2,4-dien-1-onelow score: 3-GlcA-28-Glc oleanolic acidfalseBanana flower polysaccharides promote immunoprotection through gut microbiota-associated host metabolic remodelingThe gut microbiota is increasingly recognized as an important mediator of the systemic effects of dietary polysaccharides, but how microbiota remodeling is associated with host metabolic responses remains poorly understood. Here, we investigated hepatic metabolic remodeling associated with the immunoprotective effects of banana flower polysaccharides (BFP) in cyclophosphamide (Cy)-induced immunosuppressed mice. Hepatic untargeted metabolomic profiling was performed to characterize metabolic alterations associated with BFP treatment and microbiota-related interventions. The resulting data provide a resource for investigating host metabolic changes associated with BFP-mediated immunoprotection and gut microbiota remodeling.2026-09-012026-08-26MTBLS15462COLNVLDHVKWLRT-QMMMGPOBSA-NCKLJMWTZIZZHCS-REOHCLBHSA-NCGJIPMVTBQUUQL-BQYQJAHWSA-NCOUZJTKJINDPEN-UHFFFAOYSA-NCHHLANXNIQPKBE-UHFFFAOYNA-NCPINTEKYWNYXNP-UHFFFAOYNA-N