Metabolite features were annotated using experimental mass-to-charge ratio (m/z), retention time, and available mass-spectral annotation information. Compound annotations were cross-referenced against public metabolite databases, including HMDB and KEGG where database identifiers were available. Additional spectral-library or compound-matching information was retained where available in the processed datasets. Metabolite name, m/z, retention time, database identifier, molecular formula, and sample abundance information were compiled into polarity-specific metabolite assignment files.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - reversed-phase-chromatography","Liquid Chromatography MS - negative - reversed-phase-chromatography","Liquid Chromatography MS - negative - reverse-phase"],"chromatography_protocol":["Metabolite separation was performed by reversed-phase liquid chromatography prior to mass spectrometric detection. Separate LC-MS/MS assays were performed for ovary, oviduct fluid, and serum samples in positive and negative ionization modes. Chromatography instrument, column model, column type, and other assay-specific chromatography information are recorded in the corresponding assay metadata files.
"],"publication":["Untargeted LC-MS/MS metabolomics of ovary, oviduct fluid, and serum from dogs supplemented with folic acid."],"submitter_affiliation":["xiamen university"],"submitter_name":["Site Luo"],"organism_part":["ovary","oviduct","blood serum","solvent"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Samples were prepared for untargeted LC-MS/MS metabolomic analysis using the laboratory sample-preparation workflow established for each biological matrix. Ovary, oviduct fluid, and serum samples were processed separately, and pooled quality-control samples were prepared from representative study samples for monitoring analytical reproducibility. The resulting extracts were subjected to LC-MS/MS analysis in positive and negative ionization modes.
"],"organism":["blank sample","Canis lupus familiaris"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15493"],"author":["ZHAO Baobao. Northwest A&F University. 2023060371@nwafu.edu.cn."],"data_transformation_protocol":["Raw LC-MS/MS data were processed to generate polarity-specific metabolomic feature tables. Detected features were organized using experimental mass-to-charge ratio (m/z), chromatographic retention time, and sample abundance values. Features from individual biological samples and pooled quality-control samples were aligned and compiled into processed metabolite tables. Positive- and negative-ion datasets were handled separately and subsequently formatted as MetaboLights metabolite assignment files.
"],"study_factor":["Treatment"],"submitter_email":["lstxmu@gmail.com"],"sample_collection_protocol":["Canine ovary, oviduct fluid, and serum samples were collected from control and folic acid-supplemented groups. Biological samples were assigned to the corresponding control or folic acid treatment group prior to LC-MS/MS analysis. Pooled quality-control samples were included for analytical quality assessment, and blank samples were included where applicable. Sample identifiers, tissue types, treatment groups, and sample types are provided in the study sample metadata.
"],"omics_type":["Metabolomics"],"study_design":["ultra-performance liquid chromatography-mass spectrometry","pooled quality control sample","Metabolomics","oviduct","Waters ACQUITY UPLC H-Class System","untargeted analysis","solvent blank","experimental sample","blank sample","ovary","Canis lupus familiaris","blood serum","TripleTOF 6600","mass spectrometry","solvent","Q Exactive HF","Q Exactive"],"curator_keywords":["ultra-performance liquid chromatography-mass spectrometry","pooled quality control sample","Metabolomics","oviduct","Waters ACQUITY UPLC H-Class System","untargeted analysis","solvent blank","experimental sample","ovary","blank sample","Canis lupus familiaris","blood serum","TripleTOF 6600","mass spectrometry","solvent","Q Exactive HF","Q Exactive"],"mass_spectrometry_protocol":["Untargeted mass spectrometry was performed in both positive and negative ionization modes. Ovary samples were analyzed using a Thermo Scientific Q Exactive HF mass spectrometer, oviduct fluid samples were analyzed using an AB SCIEX TripleTOF 6600 mass spectrometer, and serum samples were analyzed using a Thermo Scientific Q Exactive mass spectrometer. Electrospray ionization was used where documented in the assay metadata. Mass spectral acquisition ranges, ionization polarity, mass analyzer, ion source, and instrument-specific information are provided in the corresponding assay metadata files.
"],"metabolite_name":["Benzoic acid","Taurine","CYCLOATE","Naringenin","p-Cresol sulfate","Zinniol","Fumaric acid (not validated)","flecainide","Cer(d18:1/23:0)","(+)-Aspidospermidine","3-(Methylthio)acryloyl-CoA; Methylthioacryloyl-CoA; 3-(Methylsulfanyl)acryloyl-CoA","PC(16:0/18:2)","Trifluoroacetic acid","Fluorenone","Oxidized Photinus luciferin","APRINDINE","PC(18:2/18:1)","O-carbamyl-dl-serine","3,5,3'-triiodothyropyruvic acid","PE(18:1/20:4)","Threonine","Aconine","PC(15:0/22:6)","3-O-Methylquercetin","N-Ethylglycine","PC(16:0/16:1)","Arsenic acid","PE(18:1/22:6)","DICHLOROMETHANE","PC(14:0/14:0)","Propynoic acid","Glutamic acid","Metaphosphoric acid","Glutathioselenol; GSSeH","Semilepidinoside A","3-Hydroxypyridine sulfate","1,3-Diarachidin","1-Hydroxy-3-methoxy-7-primeverosyloxyxanthone","edaravone","N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole","1,7-di-O-phosphonohept-2-ulose","N-Acetylornithine","PE(P-16:0/20:3)","GDP-4-amino-4,6-dideoxy-alpha-D-mannose; GDP-alpha-D-perosamine","PE(22:5/22:6)","9-mercapto-11,17-dioxoandrosta-3,5-dien-3-yl acetate","NCIOpen2_008042","Pyroglutamic acid","CE(DiMe(9,5))","PC(15:0/16:0)","FENSULFOTHION","U 6796","Alanine","Isonicotinoyl-NADP adduct; INH-NADP adduct","Phenylalanine","4'-Chloroacetanilide","LPA(12:0)","Amaranthin","5-Amino-4-chloro-2-(5-hydroxymuconoyl)-3(2H)-pyridazinone; 5-Amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone","PC(14:0/16:1(9Z))","PC(14:0/18:3(9Z,12Z,15Z))","Xanthine","Caffeic acid","Arsonoacetic acid","Ginkgolide B","Cefazolin","(Z)-5-Hexadecenoic acid","2-(4-Chloro-3,5-dimethylphenoxy)-N-(2-phenyl-2H-benzotriazol-5-yl)-acetamide","ketamine","PE(16:0/18:3(9Z,12Z,15Z))","PC(15:0/16:1)","Solasonine","MK826","Pantothenic acid","PE(20:3/22:6)","PS(22:6/22:6)","1,2,3,6-Tetragalloylglucose","Tripoxyrollin","2-(Methylthio)benzothiazole","Perfluorododecanoic acid","Diflunisal","(3-hydroxy-2-octadeca-9,12-dienoyloxypropyl) octadeca-9,12-dienoate","Diallyl tetrasulfide","SCHEMBL4364428","Cer(d18:1/24:1)","3-(4-hydroxy-3,5-diiodophenyl)-2-oxopropanoic acid","Shikimic acid","dITP","PC(16:1/16:1)","Acalyphin","THIOACETAMIDE","1,6-DINITROPYRENE","Tolytoxin","IDP","fentanyl","PC(14:0/18:2)","Phenyl vinyl sulfide","Pelargonidin 3-O-3'',6''-O-dimalonylglucoside","Theacitrin A","PC(14:0/16:0)","Octaethylene glycol","Hydriodic acid","PE(P-18:1/22:4)","Pelargonidin 3-glucoside","Tyrosine","Isoplumbagin","Neobetanin","2-amino-4-oxo-4-phosphonooxybutanoic acid","Methcillin","Cer(d18:1/22:0)","2-Amino-5-chloromuconate 6-semialdehyde","Uridine, trisodium salt","CHEBI:82195","1,2,4,5-Tetrathiane","thiotepa","4-Hydroxyquinoline","2-Hydroxy-2-methylbutyric acid","hypotaurine","Peroxynitrous acid","Succinic acid","Hypoxanthine","Oxypurinol","PC(14:0/16:1)","N-(2R-Hydroxytricosanoyl)-2S-amino-1,3S,4R-octadecanetriol","Jamaicamide C","Rugulosin","mollicellin F","CHEMBL29141","3-Deazauridine","PC(14:0/20:4)","Cer(d18:1/25:0)","SULFURIC ACID","busulfan","Azelaic acid","lysylhistidine","emedastine","PE(P-18:0/18:2)","Iprobenfos","SCHEMBL10001351","CAIR; 4-Carboxy-AIR","PE(20:5/22:6)","Fasciculic acid C","PE(P-18:1/22:6)","Thiamine","CHLOROPRENE","4-Keto-anhydrotetracycline; 4-Ketoanhydrotetracycline; 4-Keto-ATC; 4-Dedimethylamino-4-oxo-anhydrotetracycline; 4-De(dimethylamino)-4-oxoanhydrotetracycline; (4aR,12aS)-3,10,11,12a-Tetrahydroxy-6-methyl-1,4,12-trioxo-4a,5-dihydrotetracene-2-carboxamide","Cilastatin","Nithiazide","Uric acid","FLAMPROP","Mangiferin 6'-gallate","PE(P-16:0/20:0)","3-(2,2-Dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid","PS(18:0/20:4(8Z,11Z,14Z,17Z))","pentamidine","Perfluorohexanesulfonic acid","PE(22:6/16:0)","PE(O-16:1(1Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))","9-fluoro-11,17-dihydroxy-10,13,17-trimethyl-1,2,6,7,8,11,12,14,15,16-decahydrocyclopenta[a]phenanthren-3-one","2,3-Dimercaptosuccinic acid","5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylic acid","3-Bromo-5-chloro-2,6-dihydroxybenzoic acid","2-ureidoglycine","Lactic acid","Archaeol","Lumichrome","1,2-Dipalmitoyl-rac-glycero-3-phosphoethanolamine","Glutamine"],"additional_accession":[]},"is_claimable":false,"name":"Untargeted LC-MS/MS metabolomics of ovary, oviduct fluid, and serum from dogs supplemented with folic acid","description":"This dataset contains untargeted metabolomic profiles of canine ovary, oviduct fluid, and serum samples collected from control and folic acid (FA)-supplemented groups. LC-MS/MS metabolomics was performed in both positive and negative ionization modes to characterize metabolic alterations associated with folic acid supplementation and to investigate systemic and reproductive-tract metabolic responses. Raw mass spectrometry data, including biological samples, pooled quality-control samples, and blank samples where applicable, are provided to support data reproducibility and downstream metabolomic analyses.","dates":{"publication":"2026-09-07","submission":"2026-08-29"},"accession":"MTBLS15493","cross_references":{"HMDB":["HMDB0000619","HMDB0004244","HMDB0000251","HMDB0004679","HMDB0000660","HMDB0304793","HMDB0000784","HMDB0003072","HMDB0000158","HMDB0005082","HMDB0001586","HMDB0001870","HMDB0000191","HMDB0000167","HMDB0003689","HMDB0000148","HMDB0002466","HMDB0005998","HMDB0000202","HMDB0000134","HMDB0000625","HMDB0010216","HMDB0000930","HMDB0011745","HMDB0304312","HMDB0000555","HMDB0032797","HMDB0000893","HMDB0000622","HMDB0000812","HMDB0255053","HMDB0001232","HMDB0000682","HMDB0000197","HMDB0000531","HMDB0255414","HMDB0000623","HMDB0000448","HMDB0000071","HMDB0010725","HMDB0000671","HMDB0000752","HMDB0006344","HMDB0002226","HMDB0251511","HMDB0002183","HMDB0000210","HMDB0249187","HMDB0000626","HMDB0000177","HMDB0000299","HMDB0000289","HMDB0000821","HMDB0005923","HMDB0000033","HMDB0000133","HMDB0000296","HMDB0000045","HMDB0000187","HMDB0000715","HMDB0034153","HMDB0000573","HMDB0015446","HMDB0002815","HMDB0002122","HMDB0001129","HMDB0010410","HMDB0000827","HMDB0002068","HMDB0035919","HMDB0244268","HMDB0001220","HMDB0061714","HMDB0002664","HMDB0002710","HMDB0000462","HMDB0000290","HMDB0304269","HMDB0000842","HMDB0002886","HMDB0060467","HMDB0000872","HMDB0006059","HMDB0002092","HMDB0041220","HMDB0249587","HMDB0011632","HMDB0004236","HMDB0001381","HMDB0040614","HMDB0000077","HMDB0251509","HMDB0000904","HMDB0028829","HMDB0256926","HMDB0001264","HMDB0000094","HMDB0030381","HMDB0241916","HMDB0032575","HMDB0247781","HMDB0002212","HMDB0028817","HMDB0013657","HMDB0013247","HMDB0000663","HMDB0059933","HMDB0003871","HMDB0304256","HMDB0006254","HMDB0041844","HMDB0246582","HMDB0000807","HMDB0000749","HMDB0244272","HMDB0002043","HMDB0000426","HMDB0032012","HMDB0032567","HMDB0001520","HMDB0032063","HMDB0244385","HMDB0000095","HMDB0029667","HMDB0303447","HMDB0240328","HMDB0006294","HMDB0000617","HMDB0033788","HMDB0001176","HMDB0000826","HMDB0000792","HMDB0001877","HMDB0011134","HMDB0006236","HMDB0000010","HMDB0000574","HMDB0002059","HMDB0303348","HMDB0004686","HMDB0302754","HMDB0030027","HMDB0000273","HMDB0028818","HMDB0031403","HMDB0000888","HMDB0038804","HMDB0001895","HMDB0001851","HMDB0000691","HMDB0250712","HMDB0002284","HMDB0000150","HMDB0000022","HMDB0015305","HMDB0013302","HMDB0010738","HMDB0004259","HMDB0015224","HMDB0000764","HMDB0000772","HMDB0029648","HMDB0000482","HMDB0011617","HMDB0001049","HMDB0002210","HMDB0000708","HMDB0000423","HMDB0000689","HMDB0000884","HMDB0029306","HMDB0000722","HMDB0000208","HMDB0000052","HMDB0001991","HMDB0244762","HMDB0003355","HMDB0257047","HMDB0000806","HMDB0000956","HMDB0000874","HMDB0250742","HMDB0040937","HMDB0000701","HMDB0000714","HMDB0259057","HMDB0004284","HMDB0059805","HMDB0258895","HMDB0002124","HMDB0034649","HMDB0000044","HMDB0004586","HMDB0060274","HMDB0000279","HMDB0062174","HMDB0002261","HMDB0036619","HMDB0000698","HMDB0001254","HMDB0001366","HMDB0029666","HMDB0001068","HMDB0000929","HMDB0002670","HMDB0000393","HMDB0000866","HMDB0001274","HMDB0000181","HMDB0029098","HMDB0303377","HMDB0000121","HMDB0060475","HMDB0304237","HMDB0003559","HMDB0040286","HMDB0000156","HMDB0003099","HMDB0002725","HMDB0259262","HMDB0000125","HMDB0000227","HMDB0252849","HMDB0000063","HMDB0000232","HMDB0000012","HMDB0000725","HMDB0001341","HMDB0006809","HMDB0246621","HMDB0003423","HMDB0000292","HMDB0002925","HMDB0003235","HMDB0000300","HMDB0001043","HMDB0062175","HMDB0002823","HMDB0255303","HMDB0003252","HMDB0011171","HMDB0000195","HMDB0028752","HMDB0000467","HMDB0036065","HMDB0000214","HMDB0003306","HMDB0000014","HMDB0259044","HMDB0000182","HMDB0304778","HMDB0000194","HMDB0242411","HMDB0000239","HMDB0000933","HMDB0000099","HMDB0000220","HMDB0011175","HMDB0002055","HMDB0010382","HMDB0059969","HMDB0000422","HMDB0000374","HMDB0252293","HMDB0000245","HMDB0000036","HMDB0000466","HMDB0255056","HMDB0251521","HMDB0002904","HMDB0000700","HMDB0000745","HMDB0035140","HMDB0011352","HMDB0000452","HMDB0005779","HMDB0011506","HMDB0007982","HMDB0007973","HMDB0008031","HMDB0012136","HMDB0002712","HMDB0004702","HMDB0112194","HMDB0002302","HMDB0001906","HMDB0008937","HMDB0009003","HMDB0001031","HMDB0000354","HMDB0000225","HMDB0131479","HMDB0004811","HMDB0004812","HMDB0006555","HMDB0001409","HMDB0000016","HMDB0000355","HMDB0000428","HMDB0000732","HMDB0000440","HMDB0000491","HMDB0001527","HMDB0001886","HMDB0000026/HMDB0062812","HMDB0000026","HMDB0002441","HMDB0001336","HMDB0039853","HMDB0062295","HMDB0001392","HMDB0031511","HMDB0035268","HMDB0136142","HMDB0029645","HMDB0000763","HMDB0001855","HMDB0006248","HMDB0001517","HMDB0012843","HMDB0001316","HMDB0130547","HMDB0006218","HMDB0014797","HMDB0059911","HMDB0000532","HMDB0000034","HMDB0000303","HMDB0062251","HMDB0006285","HMDB0003345/HMDB0061922","HMDB0000019","HMDB0001147","HMDB0001123","HMDB0015605","HMDB0000151","HMDB0032133","HMDB0000039","HMDB0033826","HMDB0000511","HMDB0000535","HMDB0000482/HMDB0062511","HMDB0002385","HMDB0014513","HMDB0030282","HMDB00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