Metabolite annotation was performed based on accurate mass to charge ratio, fragment ion patterns and retention time, matched against public databases including HMDB, METLIN and mzCloud. Compound identification was conducted with mass tolerance set at 15 ppm for precursor ions and 0.05 Da for fragment ions.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Samples were separated using an Agilent 1290 Infinity LC ultra high performance liquid chromatography (UHPLC) system equipped with a HILIC chromatographic column. The column temperature was set at 25 °C, the flow rate was 0.5 mL/min, and the injection volume was 2 μL. Mobile phase A consisted of water supplemented with 25 mM ammonium acetate and 25 mM ammonia water, while mobile phase B was acetonitrile. The gradient elution program was performed as follows: 0–0.5 min, 95% B; 0.5–7 min, a linear decrease of B from 95% to 65%; 7–8 min, a linear decrease of B from 65% to 40%; 8–9 min, B maintained at 40%; 9–9.1 min, a linear increase of B from 40% to 95%; 9.1–12 min, B maintained at 95%. During the whole analytical run, samples were kept in an autosampler at 4 °C. To eliminate biases caused by instrumental signal fluctuations, all samples were analyzed consecutively in a random order. Quality control (QC) samples were inserted into the sample queue to monitor and evaluate system stability and the reliability of experimental data.
"],"publication":["Multi-omics reveals the response and adaptation of Acidithiobacillus ferrooxidans BY-3 under arsenic stress."],"submitter_name":["Wanting Wang"],"submitter_affiliation":["Henan Medical University"],"organism_part":["Bacterial cells"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The samples were thawed slowly at 4 °C. An appropriate amount of each sample was added into pre cooled methanol acetonitrile aqueous solution (2:2:1, v/v), followed by vortex mixing and low temperature sonication for 30 min. After standing at −20 °C for 10 min, the mixture was centrifuged at 14 000 g and 4 °C for 20 min. The supernatant was collected and vacuum dried. Prior to mass spectrometry analysis, the dried residue was reconstituted in 100 μL acetonitrile aqueous solution (acetonitrile: water = 1:1, v/v), vortex mixed, and centrifuged at 14 000 g and 4 °C for 15 min. The resulting supernatant was transferred for injection and subsequent analysis.
"],"organism":["Acidithiobacillus ferrooxidans"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15537"],"author":["Qiyu Gao. Henan Medical University. gaog345@163.com.","Wanting Wang. Henan Medical University. 17639308362@163.com."],"data_transformation_protocol":["The raw data were converted to .mzXML format using ProteoWizard software. Peak alignment, retention time correction and peak area extraction were subsequently performed with the XCMS package. Metabolite structural identification and data pre processing were firstly carried out on the XCMS generated datasets, followed by quality assessment of experimental data, and final data analysis.
"],"study_factor":["Treatment"],"submitter_email":["17639308362@163.com"],"sample_collection_protocol":["The strain was cultured in 9K liquid medium, with the initial pH adjusted to 2.0–2.2, and incubated in a rotary shaker at 180 r/min and 30 °C. When the culture reached the logarithmic growth phase (approximately 48 h), the cells were harvested by centrifugation at 8,000 × g and 4 °C for 10 min. The supernatant was discarded, and the cell pellets were washed twice with pre-cooled 0.15 mol/L NaCl solution to remove residual medium and jarosite precipitates. The resulting cell pellets were collected for subsequent experiments.
"],"omics_type":["Metabolomics"],"study_design":["Acidithiobacillus ferrooxidans","Metabolomics","metabolomic","Arsenic","untargeted analysis","transcriptomic","proteomic","experimental blank","bioleaching","Agilent 1290 Infinity HPLC","AB SCIEX TripleTOF 6600","Bacterial cells"],"curator_keywords":["Acidithiobacillus ferrooxidans","Metabolomics","metabolomic","Arsenic","untargeted analysis","transcriptomic","proteomic","experimental blank","bioleaching","Agilent 1290 Infinity HPLC","AB SCIEX TripleTOF 6600","Bacterial cells"],"mass_spectrometry_protocol":["The primary and secondary stage mass spectra of samples were acquired using an AB Triple TOF 6600 mass spectrometer. After separation on an Agilent 1290 Infinity LC ultra high performance liquid chromatography (UHPLC) system, the samples were subjected to mass spectrometric analysis on a Triple TOF 6600 instrument (AB SCIEX) with electrospray ionization (ESI) in both positive ion and negative ion detection modes.
The ESI source parameters were set as follows: nebulizer gas (Gas 1): 60, heater gas (Gas 2): 60, curtain gas (CUR): 30 psi, ion source temperature: 600 °C, ion spray floating voltage (ISVF): ±5500 V for positive and negative modes; the mass to charge ratio range for MS1 acquisition was 60–1000 Da, and the range for MS2 fragment ion acquisition was 25–1000 Da. The accumulation time for the MS1 scan was 0.20 s/spectra, and the accumulation time for the MS2 scan was 0.05 s/spectra. MS2 spectra were obtained in the information dependent acquisition (IDA) mode with peak intensity based screening. The declustering potential (DP) was ±60 V for positive and negative modes, and the collision energy was set at 35 ± 15 eV. The IDA parameters were configured as follows: the dynamic exclusion isotope ion width was 4 Da, and 10 fragment spectra were acquired per scan.
"],"metabolite_name":["D-myo-inositol-1,4-diphosphate","L-Fucose-1-phosphate","Riboflavin","3,5-di-tert-butyl-4-hydroxybenzoic acid","Poricoic acid a","Oxyresveratrol","Methyl hexadecanoate","Deoxyinosine","Ciprofloxacin piperazinyl-n4-sulfate","N-acetyl-p-fluoro-dl-phenylalanine","Cortisol 21-sulfate","Epimedin a","Reserpine","5'-o-acetyladenosine","Lauroyl-coa","O-Acetyl-L-serine","Coenzyme a (coa)","Cauloside c","Pi(18:0/16,17-epdpe)","Fa 18:1+1o","1h-imidazo[4,5-c]pyridine-6-carboxylic acid, 1-[[4-(dimethylamino)-3-methylphenyl]methyl]-5-(2,2- diphenylacetyl)-4,5,6,7-tetrahydro-, (6s)-","D-pinitol","L-Sorbose","Neochlorogenic acid","Octanoic acid, 2-[[4-[([1,1'-biphenyl]-4-ylmethyl)amino]-6-chloro-2-pyrimidinyl]thio]-","Fumagillin","Kukoamine a","NCGC00169093-01","L-methionine","3',5'-cyclic damp","D-Arabinono-1,4-lactone","Cannabinol","D-erythro-sphinganine-1-phosphate","Maltitol","Pg 34:2","Mevalonic acid","Oleic acid","1-o-hexadecyl-2-o-acetyl-sn-glyceryl-3-phosphoryl(n,n,n-trimethyl)hexanolamine","Glutaric acid","N-acetyl-l-aspartic acid","2-hydroxy-6-methylquinoline-3-carbaldehyde","6-[4,5-dihydroxy-6-methyl-3-[(2s,3r,4r,5r,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]-5,7-dihydroxy-2-(4-hydroxyphenyl)chromen-4-one","D-ribose 1-phosphate","Cis,cis-muconic acid","Tetrahydrocorticosterone","Cinchonine","2,2'-methylene-bis(6-tert-butyl-4 methylphenol)","1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-n,n-dimethyl","Dl-4-hydroxy-3-methoxymandelic acid","Pseudouridine","L-phenylalanyl-L-proline","Phomopsin a","Adenosine 5'-phosphosulfate (aps)","1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine","2-Dehydro-3-deoxy-D-gluconate","6-phosphogluconic acid","alpha-D-Galactose 1-phosphate","Pinostrobin","1-naphthoic acid","3'-o-desmethyletoposide","Glycerol 3-phosphate","Vindoline","Guanosine","4-pyridoxic acid","Ribothymidine","(2r,3s,4s,5r,6r)-2-[[(2s,3r,4r)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxymethyl]-6-[4-(4-hydroxyphenyl)butan-2-yloxy]oxane-3,4,5-triol","Citrate","Leu-Val","5-(4-hydroxyphenyl)-5-phenylhydantoin","1h-indole-3-propanoic acid","3-[n,n-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid","Doxorubicin","Bromadiolone","9-oxo-10(e),12(e)-octadecadienoic acid","2,6-di-tert-butyl-4-methoxyphenol","(3s,4r)-7-hydroxy-3-[(3s,4r)-7-hydroxy-4-(4-hydroxyphenyl)-2-oxo-5-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,4-dihydrochromen-3-yl]-4-(4-hydroxyphenyl)-5-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,4-dihydrochromen-2-one","Pi 40:8","N-acetyl-l-glutamate","Hexadecanedioic acid","Arenobufagin","15-ketoiloprost","L-Fucose","cis-Aconitate","D-(+)-mannose","Nname,cis-9,10-Epoxystearic acid","N-acetyl-d-glucosamine 6-phosphate","L-threonate","Ala-Ala","Uridine","(2e,4e)-12-[(10e,12e)-13-carboxy-3-hydroxy-2-(hydroxymethyl)-8,10,12-trimethyltrideca-10,12-dienoyl]oxy-13-(hydroxymethyl)-3,5,7-trimethyltetradeca-2,4-dienedioic acid","Nilotinib","Malonic acid","S4:18(p3:16/f1:2)","Proline","1-palmitoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)","Phosphonic acid, p-[[4-[(1-oxotetradecyl)amino]phenyl]methyl]-","Avocadyne","Fumonisin b1","Oligomycin b","Bafilomycin b1","Pg 36:2","L-fucitol","Oligomycin a","Thr-Ala","5-methyltetrahydrofolic acid","Aspartic acid","Gdp-l-fucose","3-methoxyprostaglandin f1.alpha.","L-Ascorbic acid","Catalposide","17,20,21-trihydroxypregn-1-ene-3,11-dione","Cochlioquinone a","D-ribulose 5-phosphate","Alpha-ketoglutarate","Linoleic acid","2,3-dinorfluprostenol","Tacrolimus","3,4-dihydroxymandelic acid","Urapidil","1-palmitoyl-3-oleoyl-sn-glycero-2-phosphoethanolamine","L-Phenylalanine","Benzenesulfonic acid, dodecyl(sulfophenoxy)-","Sucrose","5-hexenoic acid, 6-[(2r,3s)-3-[[[(4-chloro-2-methylphenyl)sulfonyl]amino]methyl]bicyclo[2.2.2]oct-2-yl]-, (5z)-","3-(1h-indol-3-ylmethyl)-6,18-dimethyl-9-(2-methylpropyl)-12-(1-phenylethyl)-15-propan-2-yl-1,4,7,10,13,16,19-heptazacyclotricosane-2,5,8,11,14,17,20-heptone","3-Hydroxycapric acid","N-acetyl-l-methionine","Fahfa 36:0","Glucose","Tetradecanedioic acid","Erythritol","D-glucono-1,5-lactone","4-hydroxybenzoate","3-[(cholamidopropyl)dimethylammonio]-1-propanesulfonate","Batimastat","Natamycin","3-hydroxymyristic acid","11-dehydrothromboxane b2","Threonine","Terephthalic acid","Isodeoxycholic acid","Polyphyllin e","Bonactin","Rauwolscine","Aphyllic acid","Thr-Thr","Uridine 5'-diphosphoglucuronic acid (UDP-D-glucuronate)","Lovastatin hydroxy acid","L-Iditol","Buprenorphine","Pentaerythritol tetrakis(3,5-di-tert-butyl-4-hydroxyhydrocinnamate)","1-oleoyl-l-.alpha.-lysophosphatidic acid","Arsenic acid","NCGC00381123-01","Astragaloside ii","Glutamic acid","(e)-5-(4-methoxy-5-methyl-6-oxopyran-2-yl)-3-methylhex-4-enoic acid","Suberic acid","5-heptenoic acid, 7-[(1r,2r,3s,5s)-2-[(1e,3s)-3-(2,3-dihydro-1h-inden-2-yl)-3-hydroxy-1-propen-1-yl]-3-fluoro-5-hydroxycyclopentyl]-, (5z)-","(r)-2-hydroxystearic acid","Dihydroergocristine","Pectolinarin","1,2-distearoyl-sn-glycero-3-phospho-l-serine","NADH","D-psicose","S-methyl-5'-thioadenosine","3-Methoxy-4-Hydroxyphenylglycol Sulfate","Udp-n-acetylglucosamine","Acetyl phosphate","[(2r,3s,4s,5r,6s)-6-[3,5-dihydroxy-4-[3-(4-hydroxyphenyl)propanoyl]phenoxy]-3,4,5-trihydroxyoxan-2-yl]methyl 3,4,5-trihydroxybenzoate","UDP-N-acetylmuraminate","Tricoumaroyl spermidine","Perseitol","Uridine 5'-monophosphate","Pitavastatin","Spinosine","(2e,6e,11e,13e)-18-(2,6-dioxopiperidin-4-yl)-9-hydroxy-8-methoxy-10,12,14-trimethyl-15-oxooctadeca-2,6,11,13-tetraenoic acid","Gentiobiose","Cytidine 5'-diphosphate","Val-Asn","Val-Asp","1-palmitoyl-2-linoleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)","Gibberellic acid","Gly-His-Lys","1,2-propanediol, 3-(1,3-benzodioxol-5-yl)-","(5e)-3,4,9-trihydroxy-2-propyl-2,3,4,7,8,9-hexahydrooxecin-10-one","N-acetylglutamine","1,2-Benzenedicarboxylic acid","Ala-asp","Phytolaccagenin","Octadecanoic acid","Pentadecanoic Acid","Tenofovir","1,2-diarachidonoyl-sn-glycero-3-phosphocholine","C10-dats","Buprenorphine glucuronide","Oseltamivir acid","alpha-Guanidinoglutaric Acid","Indole-3-carboxaldehyde","3'-hydroxyrepaglinide","D-Alanyl-D-alanine (D-Ala-D-Ala)","4'-hydroxydiclofenac","Pyrrolidinium, 1-[(7r)-7-(acetyloxy)-4-hydroxy-4-oxido-3,5,9-trioxa-4-phosphapentacos-1-yl]-1-methyl-, inner salt","Ginsenoside rg2","Thymol-beta-d-glucoside","1-palmitoyl-2-lauroyl-sn-glycero-3-phosphorylcholine","Enrofloxacin","Cytidine","Borrelidin","Phe-val","Deoxythymidine 5'-phosphate (dTMP)","[(2r,3s,4s,5r,6s)-6-[4-[(e)-3-(2,4-dihydroxyphenyl)-3-oxoprop-1-enyl]phenoxy]-3,4,5-trihydroxyoxan-2-yl]methyl (e)-3-(4-hydroxyphenyl)prop-2-enoate","Acetyl coenzyme a","L-Glutamate","4'-methoxyflavone","Asiatic acid","Adenosine 5'-phosphosulfate","Pulchinenoside a","Dodecanoic acid","Hexosyl lpe 16:0","Uridine 5'-diphosphate","Acetic acid, 2-[4-[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propoxy]phenoxy]-","Isopimaric acid","Lactobionic acid","L-Arabinono-1,4-lactone","His-Lys","His-ser","Ginsenoside rf","Glutathione, oxidized","Clausarin","Hederasaponin b","1h-indazole-1-pentanoic acid, 3-[[[1-(aminocarbonyl)-2-methylpropyl]amino]carbonyl]-","4-hydroxybenzenesulfonic acid","Cornuside","Guanosine 5'-monophosphate","Kendomycin","4-hydroxy-l-glutamic acid","D-Ribulose 1,5-bisphosphate","D-Ribose","Glucuronate","Sarcosine","5,7-dihydroxy-2-phenyl-6-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]-8-(3,4,5-trihydroxyoxan-2-yl)chromen-4-one","Progesterone","16-Hydroxypalmitic acid","3'-galactosyllactose","Embelin","9-(2,3-dihydroxypropoxy)-9-oxononanoic acid","Arachidic acid","Zinniol","1-hexadecyl lysophosphatidic acid","2-C-Methyl-D-erythritol 2,4-cyclodiphosphate","D-Ribose 5-phosphate","3-Phosphoserine","3,4-dihydroxy-5-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-[[4-(2-hydroxypropan-2-yl)cyclohexene-1-carbonyl]oxymethyl]oxan-2-yl]oxybenzoic acid","3,4-dihydroxycinnamic acid (l-alanine methyl ester) amide","Pyridoxal","Succinate","2,8-quinolinediol","1,2-dipalmitoleoyl-sn-glycero-3-phosphoethanolamine","Porphobilinogen","Garcinol","2'-deoxycytidine 5'-monophosphate","2-linoleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamine","3-Hydroxydodecanoic acid","2,4-di-tert-butylphenol","1,2-dimyristoyl-sn-glycero-3-phospho-l-serine","6'-sialyl-n-acetyllactosamine","Clavulanic acid","D-Mannose","Astragaloside iii","Fructose 1-phosphate","5,6,7-trimethoxycoumarin","Alternariol","Antipain","Ile-Pro","Dinor-12-oxophytodienoic acid","1,2-dioleoyl-sn-glycero-3-phosphate","L-Tryptophan","2-keto-3-deoxyoctonic acid","Dl-4-hydroxy-2-ketoglutarate","[(1s,4as,6s,7r,7as)-4-methoxycarbonyl-7-methyl-1-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-1,4a,5,6,7,7a-hexahydrocyclopenta[c]pyran-6-yl] (1s,4as,6s,7r,7as)-6-acetyloxy-7-methyl-1-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-1,4a,5,6,7,7a-hexahydrocyclopenta[c]pyran-4-carboxylate","Neohesperidose","Methyl (5z)-5-[2-(4-hydroxy-3-methoxybenzoyl)oxyethylidene]-4-[2-[2-(4-hydroxyphenyl)ethoxy]-2-oxoethyl]-6-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4h-pyran-3-carboxylate","Phenylalanine","N-arachidonoyl-3-hydroxy-.gamma.-aminobutyric acid","1-methyluric acid","Blood group b trisaccharide","Galactinol","4-hydroxyvalsartan","Testosterone","Fumarate","(+)-catechin","Chorismic acid","N-Acetylmannosamine","L-Valine","2-docosahexaenoyl-1-stearoyl-sn-glycero-3-phosphoserine","1,4-bis[(p-hydroxyphenethyl)amino]anthraquinone","Pepstatin a","5-[(z)-14-(3,5-dihydroxyphenyl)tetradec-10-enyl]benzene-1,3-diol","Rubranoside a","Acetoxyvalerenic acid","(2e,6e,12e)-18-(2,6-dioxopiperidin-4-yl)-9,11-dihydroxy-8-methoxy-10,12,14-trimethyl-15-oxooctadeca-2,6,12-trienoic acid","Fungichromin","Fahfa 34:1","Maltotetraose","1',3'-bis[1,2-dilinoleoyl-sn-glycero-3-phospho]-sn-glycerol","Acetyl coenzyme A (Acetyl-CoA)","Ostruthin","1h-indole-1-pentanoic acid, 3-[(4-methoxy-1-naphthalenyl)carbonyl]-","Creatine","Cytidine 5'-monophosphate",".beta.-nicotinamide adenine dinucleotide(NAD)","1-methylguanosine","3-Methyluridine","N-stearoyltaurine","N-acetylmuramic acid","Valerenic acid","Harpagoside","L-homocysteic acid","(19r)-9-acetyl-19-hydroxy-10,14-dimethyl-20-oxopentacyclo[11.8.0.0<2,10>.0<4,9 >.0<14,19>]henicos-17-yl acetate","4-hydroxybenzaldehyde","Acetyl-coa","2-[[5-[[2,12-dihydroxy-4,4,10,13,14-pentamethyl-17-(1,5,6-trihydroxy-6-methylheptan-2-yl)-2,3,5,6,7,11,12,15,16,17-decahydro-1h-cyclopenta[a]phenanthren-3-yl]oxy]-3-hydroxy-3-methyl-5-oxopentanoyl]amino]acetic acid",".alpha.-solanine","Thymidine 3',5'-cyclic monophosphate","Myristic acid","Pregnenolone sulfate","m-Chlorohippuric acid","11-ketofusidic acid","Adipic acid","(S)-2-Hydroxyglutarate","5,8,11,14-eicosatetraenamide, n-(4-hydroxy-2-methylphenyl)-, (5z,8z,11z,14z)-",".alpha.-d-galactose 1-phosphate","Hymeglusin","D-Allose","N-acetyltryptophan","2-keto-l-gulonic acid","1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)","Indinavir","Isoleucine","Dicloxacillin","Methyl (3r,4r,5r)-5-[(2r,3r,4s,5s,6r)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(e)-3-phenylprop-2-enoyl]oxyoxan-2-yl]oxy-3,4-dihydroxycyclohexene-1-carboxylate","G1s neuac(2-3) a","Eplerenone hydroxy acid","Glutaconic acid","Adenine","Canrenone","Pyruvate","D-arabinose","16,16-dimethyl-6-ketoprostaglandin e1","3-hydroxyanthranilic acid","Pi 38:6","D-Proline","D-quinovose","Hydroquinidine","Daunomycinone","Inosine","3',5'-Cyclic guanosine monophosphate","11-oxomogroside v","Trans-epoxysuccinyl-l-leucylamido(4-guanidino)butane","Glutamine","Andrastin a","N-Acetyl-L-phenylalanine","1-(10z-heptadecenoyl)-sn-glycero-3-phospho-(1'-rac-glycerol)","Dihydroxyacetone","Dihydroxyacetone phosphate","Lamiide","Cucurbitacin i","Soyasaponin i","Procyanidin c1","Palmitic acid",".gamma.-glutamyl-(s)-allyl-l-cysteine","7-methoxy-6-(1,2,3-trihydroxy-3-methylbutyl)chromen-2-one","Voacamine","Gentiopicroside","Flavin mononucleotide (fmn)","Raltitrexed","alpha,alpha-Trehalose","4'-demethylpodophyllotoxin","N-nitroso-n-methyl-3-aminopropionic acid","Caffeine","D-gluconate","Andrastin d","Hypoprotocetraric acid","4-acetamidobutanoate","Microcystin la","18-hydroxy-5z,8z,11z,14z,16e-eicosapentaenoic acid","Diprotin b","N-acetylserine","Maltotriose","Gly-Val","(z)-5,8,11-trihydroxyoctadec-9-enoic acid","Dihydrouracil","Linustatin","Formononetin","Dienogest","3,4-dichlorophenol","Dl-lactate","L-alaninamide, n-[2-[2-(hydroxyamino)-2-oxoethyl]-4-methyl-1-oxopentyl]-3-methyl-l-valyl-n-(2-aminoethyl)-","1-deoxy-d-xylulose 5-phosphate","[3-hydroxy-1-(4-methoxy-7-oxofuro[3,2-g]chromen-9-yl)oxy-3-methylbutan-2-yl] (e)-2-methylbut-2-enoate","D-lyxose","1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine","Dinorprostaglandin e1","Salidroside","1-myristoyl-sn-glycero-3-phosphocholine","S-adenosyl-l-homocysteine","2-[3-[(e)-6-acetyloxy-2-hydroxy-6-methyl-3-oxohept-4-en-2-yl]-6-(carboxymethyl)-2-hydroxy-3a,5a,9b-trimethyl-5-oxo-2,3,4,6,9,9a-hexahydro-1h-cyclopenta[a]naphthalen-7-yl]-2-methylpropanoic acid",".alpha.,.alpha.'-trehalose 6-phosphate","Thiamine pyrophosphate (tpp)","Uridine 5'-diphosphate (UDP)","Medicagenic acid base + o-hex","Pi(16:1/13-hode)","Udp-galactose","(2-acetyloxy-3,6-diphenylcyclohexyl) acetate","L-Pyroglutamic acid","N-acetyl-d-galactosaminitol","Benzoic acid, 3-[[(3-carboxycyclohexyl)amino]carbonyl]-4-[3-[4-(4-phenoxybutoxy)phenyl]propoxy]-","Gamma-Glu-Cys","Salicylic acid","1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine","1-Hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoric acid","Dodecanedioic acid","M-hydroxycocaine","Isomaltose","Leiocarposide","Xanthine","ADP-ribose","L-Glutamine","Deoxyguanosine","3-isoxazolecarboxamide, 5-(5-chloro-2,4-dihydroxyphenyl)-n-ethyl-4-(4-methoxyphenyl)-","N-acetyl-d-mannosamine","N-Acetyl-L-alanine","L-gulono-1,4-lactone","Pi 32:0","Thymidine","Labetalol","D-arabinonic acid","L-Isoleucine","D-neopterin","Vitexin","8(9)-epoxy-5z,11z,14z,17z-eicosatetraenoic acid","1-[4,5-dihydroxy-6-(hydroxymethyl)-3-[(e)-3-(4-hydroxyphenyl)prop-2-enoyl]oxyoxan-2-yl]oxy-6-hydroxy-7-methylidene-4a,5,6,7a-tetrahydro-1h-cyclopenta[c]pyran-4-carboxylic acid","Glucotropaeolin","Pantetheine","Methylmalonic acid","1,2-didocosahexaenoyl-sn-glycero-3-phosphocholine",".alpha.-guanidinoglutaric acid","Hexsose + hexsose-deoxyhexose + c20h32","Ltc4-[d5]","Phosphorylcholine","Maltose","2-Keto-D-gluconic acid","Glycyl-L-leucine","Swertiamarin","Decahydrogambogic acid","Pg 32:1","Hypocrellin a","Indole-3-butyric acid","Pyruvaldehyde","D-arabitol","5'-Deoxyadenosine","Sedoheptulose","Phosphonic acid, p-[2-[3-[[(1-benzoyl-4-piperidinyl)methylamino]carbonyl]-2-naphthalenyl]-1-(1-naphthalenyl)-2-oxoethyl]-","2-Oxoadipic acid","Pterine","Parabanic acid","D-fructose","Nsc 107654","D-glucose 6-phosphate","16-hydroxyhexadecanoic acid","Fosfomycin","Citramalic acid","Raffinose","1-stearoyl-2-linoleoyl-sn-glycero-3-phosphoethanolamine","[(2r,3s,4s,5r,6r)-3,4-dihydroxy-6-[(2s,3s,4s,5r)-3-hydroxy-2,5-bis(hydroxymethyl)-4-[(e)-3-(4-hydroxyphenyl)prop-2-enoyl]oxyoxolan-2-yl]oxy-5-[(e)-3-(4-hydroxyphenyl)prop-2-enoyl]oxyoxan-2-yl]methyl (e)-3-(4-hydroxyphenyl)prop-2-enoate","Cis-9-palmitoleic acid","Carbenoxolone","Desferrioxamine d2","Azelaic acid","Carboxyphosphamide","Curcumin","N-palmitoyltaurine","Aminomethylphosphonic acid","16-phenyltetranorprostaglandin f2.alpha.","Quinolinate","Ser-Leu-Ile-Gly-Lys-Val-Amide","D-Tagatose","Adenosine 3',5'-cyclic monophosphate","Celecoxib","Phosphocreatine","(2r,4as,6ar,6as,14as,14br)-10,11-dihydroxy-2,4a,6a,6a,14a-pentamethyl-8-oxo-1,3,4,5,6,13,14,14b-octahydropicene-2-carboxylic acid","(2e,6e,10e)-13-[(2r)-6-hydroxy-2,8-dimethyl-3,4-dihydrochromen-2-yl]-2,6,10-trimethyltrideca-2,6,10-trienoic acid","Xylitol","Deoxycytidine","Vitamin c","1,2-dilauroyl-sn-glycero-3-phosphatidylcholine","Mestranol","(4ar,5r,6as,6br,10s,12ar)-10-[(2r,3r,4r,5s,6r)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-5-hydroxy-2,2,6a,6b,9,9,12a-heptamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylic acid"],"additional_accession":[]},"is_claimable":false,"name":"Multi-omics reveals the response and adaptation of Acidithiobacillus ferrooxidans BY-3 under arsenic stress","description":"Acidithiobacillus ferrooxidans exhibits arsenic resistance and plays a significant role in biometallurgy and sulfur cycling within ecosystems. This study employed integrated transcriptomic, proteomic, and metabolomic analyses to investigate the molecular response mechanisms of Acidithiobacillus ferrooxidans BY-3 (At.f BY-3) under arsenic stress. It also explored its ability to bioleach heavy metals and provided new insights for bioremediation. Transcriptomic data revealed that the number of differentially expressed genes increased in an arsenic dose-dependent manner. Under arsenic stress, At.f BY-3 exhibited global downregulation of genes associated with secondary metabolism and chemotaxis, which may reduce unnecessary energy expenditure and redirect metabolic resources to arsenic resistance. Proteomic analysis identified 337 significantly differential proteins, which were mainly enriched in c-di-GMP signal transduction, sulfur cycling, redox homeostasis, and transmembrane transport. A total of 53 differential metabolites screened via metabolomics were primarily annotated to ABC transport systems, sulfur metabolism, and stress signaling. Multi-omics integration further revealed that At.f BY-3 maintained energy homeostasis via coordinated carbon and sulfur metabolism, enhanced arsenic efflux by activating ABC transporters, and optimized membrane structure and signal systems for stress perception, with sulfur cycling serving as the core arsenic-adaptive strategy. Moreover, bioleaching assays confirmed the strong arsenic bioleaching capacity of At.f BY-3, which was further improved after domestication. The present work clarifies the molecular mechanisms conferring arsenic resistance to Acidithiobacillus ferrooxidans, laying a theoretical foundation for the improvement of biohydrometallurgy and heavy metal bioremediation protocols.","dates":{"publication":"2026-09-03","submission":"2026-09-03"},"accession":"MTBLS15537","cross_references":{}}