MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/m_MTBLS15597_Tetracera_poggei_metabolite_profiling_water_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/a_MTBLS15597_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/s_MTBLS15597.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/DERIVED_FILES/Tetradenia_riparia_methanol_n.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/DERIVED_FILES/Tetracera_poggei_methanol_n.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/DERIVED_FILES/Tetradenia_riparia_water_n.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/DERIVED_FILES/Tetracera_poggei_water_n.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/RAW_FILES/Tetracera_poggei_methanol_n.raw.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/RAW_FILES/Tetradenia_riparia_methanol_n.raw.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/RAW_FILES/Tetracera_poggei_water_n.raw.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597/FILES/RAW_FILES/Tetradenia_riparia_water_n.raw.zipprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15597<p>PCompounds were tentatively identified by generating the respective molecular formula from MassLynx V 4 and comparing their fragmentation patterns with that of matching compounds from various search databases including Dictionary of Natural Products, PubChem, KNApSAcK Core System and MetFrag.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phase<p>A Waters Acquity UPLC System equipped with a binary solvent delivery system and an autosampler was used to run the samples. The Chromatographic separation was achieved on a Waters BEH C18 (2.1 mm x 100 mm, 1.7 μm) column with a gradient elution of solvents A (water + 0.1% formic acid) and B (methanol + 0.1 % formic acid), applied as follow: 0 min 3% B, 0.10 min 3% B, 14 min 100% B, 16 min 100% B, 16.5 min 3% B, 20 min 3% B. The flow rate was set at 0.3 ml/min. The injection volume was 5 μl. </p>Antioxidant Activity, Phytochemical Profiling by UPLC-QTOF-MS, and In Silico Targeting of the RNA-Binding Protein DRBD18 of Trypanosoma brucei by Bioactive Compounds from Tetradenia riparia and Tetracera poggei.University of KinshasaSephora Miandaleafmass spectrometry assay<p>Extracts were obtained through decoction and percolation. For the decoction, 10 g of powdered material was suspended in 100 mL of distilled water and heated to 100 °C for 10 minutes. After cooling, the mixture was filtered using Whatman filter paper. For the percolation procedure, 10 g of powder was cold-macerated in 50 mL of a 1:1 (v/v) dichloromethane–methanol mixture for 48 hours, after which the extract was collected. The resulting extracts were evaporated to dryness and preserved at 4 °C. The dried extracted (1 mg) were re-dissolved in 1 mL MeOH/water (1:1) and filtered through a 0.22 µm nylon syringe filter before being injected into the UPLC-MS instrument.</p>Tetradenia ripariaTetracera poggeihttps://www.ebi.ac.uk/metabolights/MTBLS15597Sephora Mianda. University of Kinshasa. sephoramianda@gmail.com.Williams Balela. University of Kinshasa. williambalela7@gmail.com.<p>No data transformation was conducted. Compounds were tentatively identified by generating the respective molecular formula from MassLynx V 4 and comparing their fragmentation patterns with that of matching compounds from various search databases including Dictionary of Natural Products, PubChem, KNApSAcK Core System and MetFrag.</p>Plant speciessephoramianda@gmail.com<p>Leaves of <em>Tetradenia riparia</em> were collected between March and April 2025 in the Ngansele district, Mont-Ngafula commune, Kinshasa, DRC (S 4°26’17.197”; E 15°17’32.812”; altitude 0 m). Leaves of <em>Tetracera poggei</em> (local names: Bojo, Monji) were collected in the Kimwenza area, Mont-Ngafula commune (S 4°27’4.251”; E 15°17’37.693”). Collected plant materials were oven-dried at 40 °C for 4 days and ground using an electric grinder. </p>Metabolomicsultra-performance liquid chromatography-mass spectrometryMetabolomicsProteoWizard msconvertuntargeted analysisMassLynxWaters ACQUITY UPLC systemleafexperimental sampledata-independent acquisitionuntargeted metabolite profilingTetradenia ripariaTetracera poggeiWaters Xevo G2 Tofultra-performance liquid chromatography-mass spectrometryMetabolomicsProteoWizard msconvertuntargeted analysisMassLynxWaters ACQUITY UPLC systemleafexperimental sampledata-independent acquisitionuntargeted metabolite profilingTetradenia ripariaTetracera poggeiWaters Xevo G2 Tof<p>The separated compounds were analysed by a Waters Synapt G2 high definition QTOF mass spectrometer, which was run in electrospray ionization negative mode. The following MS source parameters were set for negative mode: source temperature 120 ºC, sampling cone 20 V, extraction cone 4.0 V, desolvation temperature 300 ºC, cone gas flow 10.0 L/h, desolvation gas flow 600 L/h, capillary 2.6 kV. It was constantly infused at a rate of 3 μl/min through a separate orthogonal ESI probe to compensate for experimental drift in mass accuracy. The trap collision energy was 28 V.</p>falseAntioxidant Activity, Phytochemical Profiling by UPLC-QTOF-MS, and In Silico Targeting of the RNA-Binding Protein DRBD18 of Trypanosoma brucei by Bioactive Compounds from Tetradenia riparia and Tetracera poggeiOxidative stress plays a key role in the pathogenesis of African trypanosomiasis, and plant-derived antioxidants represent a promising source of dual-action compounds capable of both scavenging reactive oxygen species and interfering with parasite survival. The present study investigates the antioxidant activity and phytochemical composition of two Central African medicinal plants, Tetradenia riparia (Hochst.) Codd and Tetracera poggei (Gilg.) and evaluates the in silico trypanocidal potential of their major constituents against the mRNA-binding protein DRBD18 of Trypanosoma brucei. Aqueous and methanolic extracts were prepared by decoction and percolation. Qualitative phytochemical screening and quantitative spectrophotometry (Folin–Ciocalteu, AlCl3) revealed rich profiles of polyphenols, flavonoids, tannins, anthocyanins, and alkaloids in both species. UPLC-QTOF-MS in negative ESI mode identified eleven compounds in T. riparia, including rosmarinic acid, luteolin, acacetin, and diterpenoid SP-II and thirteen compounds in T. poggei, dominated by galloylated catechin derivatives (epi-gallocatechin 3-O-gallate, catechin 3-O-gallate, (−)-epicatechin-3-(3-O-methyl)- gallate). Antioxidant activity by DPPH and ABTS radical scavenging assays showed that percolated extracts consistently outperformed decocted extracts; T. poggei percolated extract showed the strongest DPPH activity (IC50 = 4.141 ± 0.175 µg/mL). Molecular docking against the RRM1 and RRM2 domains of DRBD18 (AlphaFold model AF-Q57XR9-F1) revealed that diterpenoid SP-II and (−)- epicatechin-3-(3-O-methyl)-gallate displayed the highest binding affinities (RRM1: −7.3 kcal/mol; Ki = 4.424 µM). The strong antioxidant capacity of the identified compounds, particularly galloylated catechins and rosmarinic acid, is mechanistically linked to their structural features that also favor RRM domain binding, highlighting a dual antioxidant antiparasitic pharmacological profile. These findings provide a rational basis for the further development of plant-derived agents targeting oxidative stress and RNA-binding mechanisms in T. brucei.2026-09-102026-09-08MTBLS15597