MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/DERIVED_FILES/metabolome_neg.xlsxftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/DERIVED_FILES/metabolome_pos.xlsxftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/DERIVED_FILES/Metabolite_Identification_Quantitative_Table.xlsxftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/m_MTBLS15610_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/m_MTBLS15610_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/s_MTBLS15610.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/a_MTBLS15610_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/a_MTBLS15610_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/CIP2_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/Ctrl3_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/Ctrl2_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/Ctrl3_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/Ctrl1_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/CIP3_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/Ctrl2_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/Ctrl1_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/CIP1_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/CIP1_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/CIP3_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610/FILES/RAW_FILES/CIP2_pos.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15610<p>Metabolite annotation was performed using HMDB, MassBank, LIPID MAPS, mzCloud, KEGG, and the in-house reference-standard spectral library maintained by Panomix.</p><p>Candidate molecular formulas and metabolite annotations were assigned using precursor-ion mass-to-charge ratios (m/z), mass accuracy, and adduct information, followed by database matching with a mass tolerance of less than 30 ppm. For features with acquired MS/MS spectra, fragment-ion information was further compared with reference spectra in the databases to support metabolite annotation.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using a Vanquish UHPLC system (Thermo Fisher Scientific, USA) equipped with an ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm; Waters, Milford, MA, USA). The column temperature was maintained at 40 °C. The flow rate was 0.3 mL/min, and the injection volume was 2 µL.</p><p>For positive-ion analysis, mobile phase A was water containing 0.1% formic acid, and mobile phase B was acetonitrile containing 0.1% formic acid. For negative-ion analysis, mobile phase A was aqueous 5 mM ammonium formate, and mobile phase B was acetonitrile.</p><p>The following gradient was used in both modes: 0–1 min, 10% B; 1–5 min, 10–98% B; 5–6.5 min, 98% B; 6.5–6.6 min, 98–10% B; and 6.6–8 min, 10% B.</p>Untargeted UPLC-MS/MS metabolomics of zebrafish gut tissue after chronic ciprofloxacin exposure.Shanghai Xin Hua HospitalNengzheng Wangintestinemass spectrometry assay<p>Approximately 100 mg of homogenized intestinal tissue was transferred into a 2 mL microcentrifuge tube and extracted with 1 mL of tissue extraction buffer. Samples were processed twice using a high-throughput tissue grinder, followed by ultrasonication for 25 min at 25 °C. The extracts were centrifuged at 12,000 rpm for 15 min. Supernatants were collected and filtered through a 0.22 µm membrane before LC-MS analysis.</p><p>A pooled quality-control (QC) sample was prepared by combining 20 µL aliquots of the individual sample extracts.</p>Danio reriohttps://www.ebi.ac.uk/metabolights/MTBLS15610Nengzheng Wang. XinHua Hospital. wangnengzheng01@163.com.<p>Raw mass spectrometry files were converted to mzXML format using MSConvert from ProteoWizard (version 3.0.8789). Data preprocessing was performed using the XCMS package (version 3.12.0) in R, including peak detection, peak filtering, and peak alignment.</p><p>The processing parameters were bw = 2, ppm = 15, peakwidth = c(5, 30), mzwid = 0.015, mzdiff = 0.01, and method = 'centWave'.</p><p>Quality-control (QC)-based support vector regression was applied to correct systematic variation. Features with a relative standard deviation (RSD) greater than 30% in the QC samples were excluded before downstream analysis.</p>Treatmentwangnengzheng01@163.com<p>Adult female zebrafish (Danio rerio, AB strain) were exposed to 5,000 ng/L ciprofloxacin for 30 days, beginning at 120 days post-fertilization. An unexposed control group was maintained in parallel. At 150 days post-fertilization, intestinal tissues were collected for untargeted metabolomic analysis. Each biological replicate comprised pooled intestinal tissues from five fish. Three pooled biological replicates were prepared per group, giving six biological samples in total. Samples were snap-frozen in liquid nitrogen and stored at −80 °C until processing.</p>MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicsLC-MSintestineuntargeted analysisDanio rerioThermo Scientific Q Exactive FocusciprofloxacinThermo Scientific Vanquish UHPLC SystemMetabolomicsLC-MSintestineuntargeted analysisDanio rerioThermo Scientific Q Exactive Focusciprofloxacin<p>Mass spectrometric detection was performed using a Q Exactive Focus mass spectrometer (Thermo Fisher Scientific, USA) equipped with an electrospray ionization source. Data were acquired separately in positive- and negative-ion modes. Spray voltages were +3.50 kV and −2.50 kV for positive- and negative-ion acquisition, respectively. The sheath gas and auxiliary gas settings were 40 and 10 arbitrary units, respectively. The capillary temperature was 325 °C.</p><p>Data were acquired in Full MS–ddMS2 mode. Full-scan MS1 spectra were collected over m/z 81–1000 at a resolving power of 70,000 FWHM. The top three precursor ions were selected for data-dependent MS/MS using higher-energy collisional dissociation (HCD). MS/MS spectra were acquired at a resolving power of 17,500 FWHM. Dynamic exclusion was enabled to reduce repeated fragmentation of the same precursor ions.</p>S-AdenosylmethioninamineSesartemin12-Ethyl-8-isobutyl-3-vinylbacteriochlorophyllide d4-NITROSODIPHENYLAMINEGlycerylphosphorylethanolamineP-CoumaraldehydeST 27_0;O6;SNebramycin factor 5DiazoxideStreptidineN-Fructosyl isoleucinedihydro-3-hydroxy-4,4-dimethyl- 2(3H)-Furanone(S)-6-O-MethylnorlaudanosolineConiferyl alcoholMacowine1-Hydroxypyrene-7,8-oxideJuvenile hormone III2-Butyne-1,4-diolLunarineKinetinCyanobenzene3,6-anhydro-alpha-L-galactopyranoseL-HistidineIndoleacetyl glutamine(S)-4-HydroxymandelonitrileCuminyl alcoholDL-XyloseDehydroabietic acidParaoxon6-Hydroxyhexanoic acidFA 20_5;O32-linoleoylglycerol (18_2)Oxoglutaric acid2-oxogluconic acidFA 20_5;O2beta-Carboline6-(1-hydroxy-3-oxobutyl)-7-methoxy-2H-chromen-2-oneL-ProlineTafenoquine(Z)-FarnesolPhysapubescinMethyl indole-3-acetateD-Psicose1-Hydroxy-2-naphthoic acid(R)-mandelic AcidN-Methylethanolaminium phosphateCysteineglutathione disulfide3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dioneArtemetindapdiamide C16-oxo-palmitate4-Hydroxybenzaldehyde3-acetyloctanalBrassicasterol9-HOTrEVernomygdin1-(2-Hydroxyethyl-2-hydroxymethyl)-5-nitro-imidazolePsychosine sulfateProstaglandin E2Prostaglandin E1PinostilbeneQuinoline-3,4-diol1,2,3-TrihydroxybenzeneN-Oleoyl-L-SerineEstradiolDihydroneopterin phosphate3,4-Dihydroxymandelic acidUbiquinone 612-Ketodeoxycholic acidDihydrochelirubinebeta-GeraniolTrioxilin A3Tetrahydrocannabinol5-hydroxyindole thiazolidine carboxylateGeraniol4-Methylaminobutyrate13-Apo-beta-carotenoneGentamicinc1AUrsodeoxycholateMoupinamideCathasterone3-IodothyronaminePseudouridine(9E,11E)-Octadecadienoic acid1,1'-(1,4-Dihydro-4-nonyl-3,5-pyridinediyl)bis[1-decanone]Caffeic acidABA-GE cpdGlutarate semialdehyde3-Oxoadipic acid24-Hydroxyglabrolide1,4,6-Heptatrien-3-one, 5-hydroxy-1,7-bis(4-hydroxyphenyl)-GlutaminylisoleucineFarnesolTrypanothione disulfideTiglylcarnitineD-Galacturonolactone6-deoxyerythronolide BMammeiginN-Acetylneuraminic acid(3E,7E)-4,8,12-Trimethyl-1,3,7,11-tridecatetraeneO-demethyl-N-demethyl-staurosporinePantothenic acidHeteroflavanone B2-Propylglutaric acidGlycerol 3-phosphateAlloepipregnanolone2-hydroxy-2-methylpropanal5-Hydroxyconiferaldehyde5,6-DihydroxyindoleCyclopropanecarboxyl-coaIsovaleric acid(4aR,10bS)-NoroxomaritidineN-(p-Hydroxyphenyl)ethyl p-hydroxycinnamide4-Coumaryl alcoholGlyceraldehyde 3-phosphateACMC-20lajdL-OrnithineCysteine S-sulfateall-trans-Hexaprenyl diphosphateUrobilinogenButyl formate(S)-3-Amino-3-phenylpropanoic acidFusidic AcidNeosaxitoxin6-methylthiohexanaldoxime6-Oxo-G418LactaldehydeCrustecdysone4-Hydroxy-1-(3-pyridinyl)-1-butanone3beta-Hydroxy-4beta-hydroxymethyl-4alpha-methylsteroidNonanoylcarnitine5-Methoxytryptaminefecosterol10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate(+)-Neomenthol2-Phosphoglyceric acidOxidized glutathionePyridineL-ThyronineCis-zeatinPyrophosphateUridine10-Apo-beta-carotenalL-argininium(1+)AndrostenedioneSerylglycine1-Deoxy-11-oxopentalenatestaurosporineHarmolol1,10-dihydro-1,10-dihydroxyfluoren-9-oneIron4-Hydroxyproline galactosideNummularine Fw Hydroxy testosterone2'-Deoxyuridine 5'-monophosphate disodium saltTridecanoic acidRibose 1-phosphateGlycerate3-ureidopropionatePaspaline BGeissoschizineL-Xylonic acid8,8a-Deoxyoleandolide13-OxoODEN-[(2s)-2-Amino-2-Carboxyethyl]-L-Glutamic AcidCaffeylaldehydeGlycoursodeoxycholic acid2-Naphtylmethylsuccinic aciddIMPN1-Acetyl-tabtoxinine-beta-lactamDesmethylxanthohumolN-AcetyltryptophanLysoPG(16_0_0_0)Uric acidIndolepyruvate5-AcetamidovaleratePC(18_2(9Z,12Z)_18_3(9Z,12Z,15Z))Homogentisic acidL-TyrosineRebimastatalpha,alpha-Trehalose 6-mycolateCiliatocholic acidSophorotriose2-HydroxyisoflavoneGinsenoside RdSucrose5,6-Epoxy-8,11,14-eicosatrienoic acidN-Acetylneuraminate 9-phosphate1-Hydroxymenth-8-en-2-oneN-alpha-acetylornithinePC(18_2(9Z,12Z)_18_1(9Z))4-Methylumbelliferyl acetateTetradecanedioic acidDocosahexaenoic acidHistidylphenylalanineNicotinic acid ribonucleoside10,11-Epoxy-3-geranylgeranylindoleDiphenoxylatePunicic_acidDihydrosanguinarine3-Dehydro-L-gulonate 6-phosphatechloric acidGibberellin A9(+)-cis-abscisic aldehydetaxa-4(20),11-dien-5-alpha-yl acetate(1S,2R)-Naphthalene 1,2-oxide7-hydroxy-4-isopropenyl-7-methyloxepan-2-oneAlnustoneTerpentetriene2,4-diacetamido-2,4,6-trideoxy-beta-L-gulopyranoseAvermectin A1a aglyconeDiisononyl phthalatePerindoprilLupuloneLinalool-8-aldehydeST 21_3;O42,3,4,6-TetrahydroxybenzophenoneCAI-1Fructosyllysineestradiol cypionateDebromohymenialdisine5-HydroxyindoleacetaldehydeN1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazoleGinsenoside Rg3TestololactoneLevoglucosanSpiropiperidineIminoerythrose 4-phosphate6,4-DihydroxyflavoneButyl propionateD-Xylulosereticulinylium(25S)-3-oxocholest-4-en-26-alBenzyloxycarbonyl-L-leucineHETISINEST 21_2;O24-Nitrophenyl butyrateCreatinineErioflorin methacrylateAKOS006369514PhaseollidinConiferinAmidinostreptamine2,3-DihydroxycarbamazepinePerseitol(R)-(E)-SulforapheneAdenosine 2',3'-cyclic phosphateCarbazoleGuanidinoacetate6-Phosphogluconic acid4-Methoxy-2,2-bipyrrole-5-carboxaldehydePropofol glucuronideAjugoseEpilubimin1,2-Dihydronaphthalene-1,2-diolPaspalinine5-Hydroxypentanoic acidGibberellin A1108-MethylnonenoateTalatizamine3-Oxoadipate enol-lactoneSolanapyrone A2-Hydroxychalcone10,11-epoxy-3,11-dimethyl-7-ethyl-2,6-tridecadienoic acidDihydrobiopterinUDP-D-FucNAc4,4-Dimethyl-5a-cholesta-8,24-dien-3-b-olCapsaicinN-acetyl-alpha-D-hexosamine 1-phosphate1-nitrosonaphthaleneTylactoneTheobromineTauroursodeoxycholic acidCortisolMurrayanolmicrothecinProtopanaxadiolNorajmalineVomilenineSolanesyl-PP10-OPDAL-Alanine7-DehydrocholesterolST 21_4;O215-keto PGI2Indole-3-carboxaldehyde(E)-2-Methylgeranyl diphosphateStearic acidalpha-CurcumeneUDP-N-Acetyl-D-galactosamineHesperetin7-Acetylintermedine2E-Dodecenedioic acidD-Fructose 2,6-bisphosphate17(R)-HDHAbeta-L-Rhamnose4-HYDROXYTRYPTAMINEPaspalineGermacrene A acidN-(Heptan-4-yl)benzo[d][1,3]dioxole-5-carboxamideS-adenosyl-4-methylthio-2-oxobutanoateBisphenol Aalpha-eudesmolN-Methylserotonin13(S)-Hydroperoxylinolenic acid1-(4-Hydroxy-3-methoxyphenyl)-3-decanoneColchicine(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate3-hydroxybenzyl alcoholN-(3-Methylbut-2-EN-1-YL)-9H-purin-6-amineUrate D-ribonucleotide(R)-AcetoinDodecanoic acidAlbaflavenonePaxillinolDiethyl phthalic acidUridine 5'-diphosphate10-Hydroxydecanoic acidNuatigenin2-dehydropantoateST 18_3;O316-OxosteroidButirosinTyrosyl-LeucineDehydrojuvabioneL-Oleandrosyl-oleandolideAcetoacetate4-Hydroxybenzyl alcoholThymineRetaspimycin10S-HpOME1,5-Bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadien-3-oneLeukoefdinAPCGossypin2,4,6-Trihydroxybenzophenone9,10-DiHOME2-Exo-hydroxy-1,8-cineole16alpha-HydroxysteroidProgesteronedihydroriboflavinDihydroclavaminate3-HydroxycarbamazepineGalactoseN-Formyl-L-glutamic acid5-Chloro-3-methylcatecholgamma-Curcumene4,4-DiaponeurosporeneTaurinePrunetinProbucol4-O-methylxanthohumol(1S,2R,4S)-Neo-dihydrocarveol7-O-Geranyl-2-O,3-dimethylflaviolinDihydrofolic acidLignoceroylcarnitineartemisinic aldehydeL-LysopineL-ThreonineDopamine 3-O-sulfateLysoPI(20_4(5Z,8Z,11Z,14Z)_0_0)27-Deoxy-5b-cyprinolfurosemideOryzalexin EFA 18_0;O3-Methyl-1-(2,4,6-trihydroxyphenyl)-1-butanoneRetronecine2-Hydroxyadipic acidOryzalexin ADroperidolOryzalexin CFA 6_2;O5L-LeucineDL-Malic acid5-Chloro-2-(3,5-di-tert-butyl-2-hydroxyphenyl)-2H-benzotriazoleFAL 8_1DrimenolPseudomoninemethyl farnesoatePorphobilinogenBiotin amideHematoporphyrin13-Desoxyterpendole I(1R,4S)-1-hydroxylimonen-2-oneIsoleucyl-IsoleucineDemissidineHepoxilin A3(-)-campheneSalicinS-(Indolylmethylthiohydroximoyl)-L-cysteine19-Hydroxyandrost-4-ene-3,17-dionetigogeninMyristoleate (14_1n5)4α-hydroxymethyl-4β-methyl-5α-cholesta-8,24-dien-3β-olUndecanoic acidα-D-Glucose-1-phosphateAmidinoproclavaminateTetrahydropalmatine2-heptylquinolin-4(1H)-oneSoyasapogenol ESventenic acid2-(10-Heptadecenyl)-6-hydroxybenzoic acidACon1_002252norlaudanosolineL-NorleucineN6-Acetylkanamycin-B14,15-Epoxyemindole SBPhosphate(±)-alpha-Narcotinebeta-1,4-Mannosyl-N-acetylglucosamine4-HydroxymethylpyrazoleManzamine A10-nonadecenoate (19_1n9)Pentadecanoic acid6-HydroxyparmomomycinCasticinPhenylalanineSerotoninCitronellyl-CoADihydrozeatinPresqualene diphosphateSuccinic acid, sulfo-, 1,4-dioctyl ester3-hydroxy-3-methylglutarateLycorineGamma-glutamylglutamineSanguinarineProlylhydroxyprolineCholesterolTestosteroneACMC-20mjc4Perampanel4,8 Dimethylnonanoyl carnitineN-[4-(3-Amino-1h-indazol-4-yl)phenyl]-n1-(2-fluoro-5-methylphenyl) ureaValylisoleucine2-Hydroxyhexadecanoic acid4-Hydroxy-3-methoxybenzenemethanolL-ValineEicosapentaenoic acid2-O-(6-Phospho-alpha-mannosyl)-D-glycerateUracil11-DehydrocorticosteroneTaurocholateLysoPA(16_0_0_0)6,7,4-TrihydroxyflavanonedemethylsuberosinST 27_0;O33-dehydroshikimateCreatinePhthalaldehydic acidNarbonolideN-Acetyl-D-mannosamine 6-phosphatePrimeverose5-Aminolevulinic acid2-MethylserineLysylvalineBromobenzene-2,3-dihydrodiolCholesterol sulfate7alpha-Hydroxypregnenolone2-Deoxy-L-ribono-1,4-lactoneFA 18_2;ONα-Acetyl-L-lysineIsobutyronitriletrans-2-DodecenoylcarnitineFenbendazole19-OxotestosteroneCinnamyl acetateKarounidiolPaliperidoneGibberellin A53DoramapimodN-acetylglucosamine_N-acetylgalactosamineMyristic acidVersicolorin B2-PhenylethanolHypoxanthineC30 botryococceneFisetinidol-4beta-olPsilocybine3,4-dihydroxy-2-methyl-4-farnesyl-3H-quinolin-1-ium-1-olatemeso-DiaminoheptanedioateL-Arginine(3S,6E,10E)-1,6,10,14-Phytatetraen-3-olFO 2546MHippuric acid4,21-dehydrogeissoschizine6-O-MethylnorlaudanosolineMyricanoneD-Glucuronate2,4'-DihydroxyacetophenoneRishitinPE(16_0_16_0)Rhizocticin A3-Amino-4,7-dihydroxycoumarinDelprostenatoTaurochenodesoxycholic acidnoroxomaritidinebeta-AlaninamideGibberellin A15CnidilinResveratrolFA 18_1;O2,4-Dihydroxy-2H-1,4-benzoxazin-3(4H)-oneKetospirilloxanthinSynaptolepis factor K1L-Glutamic acid15H-11,12-EETAXanthopterin-B2indicine5-dodecenoate (12_1n7)2(3H)-BenzothiazolethioneHeneicosylic acidAdenine6-Chloro-N-(1-methylethyl)-1,3,5-triazine-2,4-diamineCholine sulfate3-Oxo-delta4-steroidFA 7_3;O4DihydropteridineOctaprenyl diphosphateAlisol A 24-acetatePC(16_0_16_0)5-Methylthioinosine1-nitronaphthalenePalmitoleic acidPC(18_1(9Z)_18_2(9Z,12Z))Heptadecanoic acid2-trans,6-trans-FarnesalCitraconic acidL-SerineArabinosylhypoxanthine(ent-6alpha,7alpha)-6,7-Dihydroxy-16-kauren-19-oic acidDoisynoestrolThiamin acetic acidTerpendole EAminoacetoneAminohydroquinoneGamma-glutamyl-L-putrescine(R)-3-Ureidoisobutyrate5-O-beta-D-MycaminosyltylactoneCAR 3_0Cholic acid[3-[2-Aminoethoxy(hydroxy)phosphoryl]oxy-2-hydroxypropyl] hexadecanoateArachidonic acidtrihomomethioninePregnenoloneO-methylandrocymbinedelta-TocotrienolYateinγ-Aminobutyric acidMoperone4-ethylamino-6-isopropylamino-1,3,5-triazin-2-olVitamin A2 aldehydetrans-1,2-Dihydrobenzene-1,2-diolMicronomicin4-Hexen-3-one4-hydroxymandelic acidPC-M6Diethylmalonic acid5-HpETEErythronolide BGamma-Linolenic acid9JHS2AVR43Tryptophyl-IsoleucineHamameloseSebacic acidL-lactyl-2-diphospho-5-guanosine24,24-Dfhvalpha-CEHC1,2,3,6-Tetragalloyl-beta-D-glucopyranose2-Formylaminobenzaldehyde2-dehydro-3-deoxy-L-rhamnonateUDP-L-iduronateN4-Acetylcytidine6-phospho-2-dehydro-D-gluconateDextrorphanFormononetin2-IMINIOPROPANOATETauro-α-muricholic acidPropynoic acidMedrysonePC(16_0_18_1(9Z)-O(12,13))5'-Phosphoribosyl-N-formylglycinamideNSC6882344-Ketomyxol(2'E,4'Z,8E)-Colneleic acid6-Hydroxymelatonin2-cis,4-trans-xanthoxin3-(3,4-dihydroxypyridinium-1-yl)-L-alanineSuccinic acid semialdehydePrephenateYakuchinone-A4-Hydroxy-3-methylbenzoic acidPyroglutamic acidHomovanillic acid (HVA)2-Nitrophenol5-Oxoavermectin 2a aglyconeResolvin E1Decaprenyl diphosphateGlucosamine 6-phosphateFrangulanineD-glycero-D-manno-Heptose 7-phosphate3-methylthiopropyl-desulfoglucosinolateGibberellin A14 aldehydeAlanylisoleucine(S)-ReticulineHydroxymethyl indol-3-yl ketoneDodecanedioic acidGamma-glutamyl-leucineMercurongermacradienolDihydrobunololLinoelaidic acid11,20-DihydroxyferruginolMarmesin4-O-beta-D-Mannopyranosyl-N-acetyl-D-glucosamine11Z-Eicosenoic acid（20_1）Ala-Glu-OH(4Z,7Z,10Z,13Z,16Z,19Z)-22-Hydroxydocosahexaenoic acidacutumineAurachin DAsparagine9-Dehydro-ProgesteronePseudoiononeMG(18_1(9Z)_0_0_0_0)xi-2,3-Dihydro-2-oxo-1H-indole-3-acetic acidFlavanone3-(2-hydroxyphenyl)propionateFlunixinThymidineL-Isoleucine5-Acetylamino-6-formylamino-3-methyluracilIndole-3-acetamide26-HydroxyecdysoneHydroxychlorobactene glucosidebeta-CitraurinLeukotriene A4Spheroidene3-(all-trans-octaprenyl)benzene-1,2-diol4a-Formyl-5a-cholesta-8,24-dien-3b-ol3-Oxododecanoic acid3-Propylmalate15beta-HydroxyprogesteroneIsobutyrylglycine(3S,5S)-Carbapenam-3-carboxylate7-MethylxanthineN-AcetylglycineBortezomib(2xi,3xi,6E)-3,7-Dimethyl-6-octene-1,2,3,8-tetrol1-Nitronaphthalene-7,8-oxideClavaminate5-Oxo-1,2-campholideAcacetinMandelic acid3-Oxo-delta5-steroid2-Furoic acidVALPROIC ACIDStaphyloxanthin18-OxooleateBucromarone2-Pyrone-4,6-dicarboxylateDiamidino-2-phenylindoleHistamineMCULE-4400997775Equilinindole-3-glycol aldehyde6,7,12,13-Tetrahydro-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazol-5-one(-)-NeoisodihydrocarveolNCI60_000330L-Aspartic acidHomodihydrocapsaicinChlorflavoninCalpain Inhibitor I2-Amino-6-(2-hydroxypropanoyl)-2,3-dihydro-1H-pteridin-4-oneN-(4-Methoxyphenyl)-all-trans-retinamide17a-EthynylestradiolNormetanephrineIsolithocholic acidL-2-Amino-3-oxobutanoic acid3-Ethylmalate5-HETEDihomo-gamma-linolenic acid(E)-3-(2,3-Dihydroxyphenyl)-2-propenoic acidp-CresolAzelaic acid(3R)-Linalool19-Norandrosteronebeta-Myrcene3,4-DihydroxyphenylacetaldehydeFA 18_3Oxidized Renilla luciferin1,2-Dihydrophthalic acid6-Acetyl-D-glucoseFenoprofen3beta-Hydroxy-17-(1H-imidazol-1-yl)androsta-5,16-dienepregn-5-ene-3,20-dionePerifosineFA 18_22-Pentanamido-3-phenylpropanoic acid4a-HydroxytetrahydrobiopterinProtoverineXylitolIndole-3-carboxylic acidAlbendazoleChrysin5-Hydroxy-L-tryptophanEstrone(all-E)-CrocetinAlbonoursintrihydroxyferuloyl spermidinefalseUntargeted UPLC-MS/MS metabolomics of zebrafish gut tissue after chronic ciprofloxacin exposureThis project provides untargeted metabolomics data of zebrafish gut tissue from adult wild-type AB strain zebrafish (Danio rerio) under control conditions and chronic ciprofloxacin (CIP) exposure at 5,000 ng/L for 30 days.2026-09-092026-09-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