The software parameters were set as follows: mass tolerance for precursor and fragment ion library searching, 5 ppm; response threshold, 5.0% relative response deviation for fragment ions; peak extraction mass tolerance, 5 ppm; identification confidence levels selected, 'A', 'B', 'C', and 'D'; adduct forms in positive ion mode, [M+H]+, [M+NH4]+, and [M+Na]+; adduct forms in negative ion mode, [M−H]−, [M−2H]−, and [M+HCOO]−; and retention time tolerance, 0.1 min.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - hilic","Liquid Chromatography MS - negative - hilic"],"chromatography_protocol":["Separation was performed on a CSH C18 column (1.7 μm, 2.1 × 100 mm, Waters, USA). For positive ion mode, mobile phase A consisted of 60% acetonitrile in water with 10 mM ammonium formate and 0.1% formic acid, and mobile phase B consisted of 90% isopropanol, 10% acetonitrile, 10 mM ammonium formate, and 0.1% formic acid. For negative ion mode, mobile phase A consisted of 60% acetonitrile in water with 10 mM ammonium formate, and mobile phase B consisted of 90% isopropanol, 10% acetonitrile, and 10 mM ammonium formate. The gradient elution was as follows: 0–2 min, 40%–43% B; 2–2.1 min, 43%–50% B; 2.1–7 min, 50%–54% B; 7–7.1 min, 54%–70% B; 7.1–13 min, 70%–99% B; 13–13.1 min, 99%–40% B; 13.1–15 min, 40% B. The flow rate was 0.4 mL/min, the column temperature was 55 °C, and the injection volume was 5 μL.
"],"publication":["Untargeted lipidomics reveals colonic lipid alterations in C57BL/6J mice with acute Toxoplasma gondii infection."],"submitter_name":["Wenjie Cheng"],"submitter_affiliation":["Yunnan Agricultural University"],"organism_part":["Colon content"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Weigh 25 mg of tissue into a 2 mL reinforced centrifuge tube, and add 2 small magnetic beads. Add two small steel beads and 800 µL of pre-cooled dichloromethane/methanol (3:1, V/V) precipitation solution, followed by 10 µL of prepared internal standard 2 to each sample. Grind using a TissueLyser for 5 min, sonicate in an ice bath for 10 min, and stand overnight at −20 °C. Centrifuge at 25,000 g at 4 °C for 15 min, transfer 600 µL of the supernatant, and dry in a freeze dryer. Reconstitute with 600 µL of lipid reconstitution solution (isopropanol:acetonitrile:water = 2:1:1) and vortex for 10 min. Sonicate in an ice bath for 10 min. Centrifuge at 25,000 g at 4 °C for 15 min. Take 20 µL from each sample and pool them as QC. Submit the prepared supernatant to the instrument operator.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15624"],"author":["Wenjie Cheng. Yunnan Agricultural University. cwj210365@163.com."],"data_transformation_protocol":["The raw mass spectrometry data were imported into software developed by BGI for MS-based data analysis, yielding a data matrix containing lipid molecular identification and quantification results. This table was then subjected to further informatic processing.
"],"study_factor":["Treatment"],"submitter_email":["cwj210365@163.com"],"sample_collection_protocol":["On day 10 post-infection with Toxoplasma gondii, C57BL/6J mice were euthanized by CO2 asphyxiation. Colonic contents were collected, snap-frozen in liquid nitrogen, and stored at −80 °C for subsequent lipidomic analysis. Twelve C57BL/6J mice were randomly divided into two groups: an infected group (CJA group, brain cyst inoculation group, n = 6) and a control group (CJN group, no brain cyst inoculation group, n = 6). Each mouse in the infected group was administered 100 μL of PBS suspension containing 50 cysts by gavage, while each mouse in the control group received an equal volume (100 μL) of PBS buffer by gavage.
"],"omics_type":["Metabolomics"],"study_design":["Colon content","Mus musculus","Toxoplasma gondii","Thermo Scientific Q Exactive","acute infection","untargeted analysis","Colonic contents","Lipidomics","Waters ACQUITY UPLC system","untargeted metabolite profiling"],"curator_keywords":["Colon content","Mus musculus","Toxoplasma gondii","Thermo Scientific Q Exactive","acute infection","untargeted analysis","Colonic contents","Lipidomics","Waters ACQUITY UPLC system","untargeted metabolite profiling"],"mass_spectrometry_protocol":["MS1 and MS2 data were acquired using a Q Exactive mass spectrometer (Thermo Fisher Scientific, USA). The mass scan range was m/z 200–2000, with a resolution of 70,000 for MS1, an AGC target of 3e6, and a maximum injection time (IT) of 100 ms. The top 3 precursor ions by intensity were selected for fragmentation, and MS2 spectra were acquired at a resolution of 17,500, an AGC target of 1e5, and a maximum injection time (IT) of 50 ms, with stepped normalized collision energy (NCE) set at 15, 30, and 45 eV. The electrospray ionization (ESI) source parameters were set as follows: sheath gas flow rate, 40; aux gas flow rate, 10; spray voltage (|kV|), 3.80 for positive ion mode and 3.20 for negative ion mode; capillary temperature, 320 °C; and aux gas heater temperature, 350 °C.
"],"metabolite_name":["LPC(20:3)","LPC(20:4)","LPC(18:1)(rep)","LPC(20:4)(rep)","LPC(20:3)(rep)(rep)","LPC(18:1)","LPE(18:0)"],"additional_accession":[]},"is_claimable":false,"name":"Untargeted lipidomics reveals colonic lipid alterations in C57BL/6J mice with acute Toxoplasma gondii infection","description":"Although Toxoplasma gondii has been recognized as an obligate intracellular parasite, the lipid metabolic alterations it induces in colonic contents during acute infection remain poorly characterized. This study generated an acute infection model using C57BL/6J mice by oral inoculation with the T. gondii ME49 strain and performed untargeted lipidomics analysis on the colonic contents collected at day 10 post-infection. Acute infection was found to significantly elevate glycerophospholipids and reduce sphingolipids, glycerolipids, and prenol lipids, leading to 136 differentially abundant lipids. Pathway enrichment analysis identified two most significantly affected pathways: choline metabolism in cancer and glycerophospholipid metabolism, with LPA(16:0) and LPC(18:1) functioning as core hub nodes. ROC analysis identified PE(20:1e_22:4), PE(16:0_22:6), Hex1Cer(m18:0_20:4), and Hex1Cer(t17:0_22:6) as potential diagnostic biomarkers. The lipid biomarkers identified provide new candidate targets for diagnosing toxoplasmosis and for host-directed therapeutic strategies
","dates":{"publication":"2026-09-10","submission":"2026-09-10"},"accession":"MTBLS15624","cross_references":{"HMDB":["HMDB0007849","HMDB0001043","HMDB0006528","HMDB0010396","HMDB0010393","HMDB0010385","HMDB0011130"]}}