MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/m_MTBLS15752_LC-MS_alternating_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/a_MTBLS15752_LC-MS_alternating_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/s_MTBLS15752.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904969_Ss1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904978_Ss10.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904974_Ss6.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904973_Ss5.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904979_Ss11.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904980_Ss12.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904970_Ss2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904976_Ss8.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904972_Ss4.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752/FILES/MLE1904977_Ss9.mzMLprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15752<p>The GC-MS raw data were imported into Masshunter quantitative software (Agilent, USA, version number: v10.0.707.0). All ion fragments were automatically identificated and integrated by using default parameters,</p><p>besides, all intergration was checked manually. The metabolite concerntration of sample was calculated according to linear regression standard curve.Further investigate the effects of ionizing radiation on the levels of short chain fatty acids in the gut brain axis of mice, as well as the therapeutic effects of probiotics on radiation exposed mice.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - hilic<p>25 mg solid sample were accurately weighed into 2 mL grinding tube and add 500 µL water containing 0.5%phosphoric acid. The sample were froze and ground at 50 Hz for 3 min, repeated twice, followed by ultrasonic for 10 min and centrifugation at 4 oC and 13000 g for 15 min. Take 200 µL out the supernatant aqueous solution into a 1.5 mL centrifuge tube, then add 0.2 mL N-butanol solvent containing internal standard 2-ethylbutyric acid (10 μg/mL) for exaction. Finally,vortexl for 10 s, ultrasound at low temperature for 10 min, followed by centrifugation at 4 oC and 13000 g for 5 min and the supernatant were carefully transferred to sample vials for analysis.</p>Effect of transplantation of irradiated mouse feces on blood short chain fatty acids in recipient mice.Fourth Military Medical University: Air Force Medical UniversityGuiqiang Zhouserummass spectrometry assay<p>Two weeks after microbiota transplantation in mice, blood was collected from the apex of the heart at 4 ℃ and 12000rpm/min</p><p><br></p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS15752Guiqiang Zhou. Fourth Military Medical University: Air Force Medical University. zgqiang0308@163.com.<p>The GC-MS raw data were imported into Masshunter quantitative software (Agilent, USA, version number: v10.0.707.0). All ion fragments were automatically identificated and integrated by using default parameters, </p><p>besides, all intergration was checked manually. The metabolite concerntration of sample was calculated according to linear regression standard curve.</p>Raditionzgqiang0308@163.com<p>Two weeks after microbiota transplantation in mice, blood was collected.</p>MetabolomicsMetabolomicsMus musculustargeted analysis8890BAgilent 7000D Triple QuadrupoleIrradiationOpenMSAnxietyserumUHPLC -Orbitrap Exploris 240experimental sampleMetabolomicsMus musculustargeted analysis8890BAgilent 7000D Triple QuadrupoleIrradiationOpenMSAnxietyserumUHPLC -Orbitrap Exploris 240experimental sample<p>The analysis was performed using an Agilent 8890B gas chromatography coupled to an Agilent 5977B/7000D mass selective detector with an inert electron impact (EI) ionization source and ionization voltage was 70eV (Agilent, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). Analyte compounds were separated with a HP-FFAP (30 m×0.25mm×0.25 µm) capillary column, using 99.999% helium as a carrier gas at a constant flow rate (1 mL/ min). The GC column temperature was programmed to hold at 80 oC and rise to 120 oC at a rate of 40 oC per minute, then rise to 180 oC at a rate of 10 oC per minute, finally rise to 200 oC at a rate of 20 oC per minute hold at temperature of 220 oC for 3 min. The injection volume of samples was 1 µL and introduced in splitting mode (10:1) with the inlet temperature of 230 oC. The ion sources temperature was 250 oC and the quadrupole temperature temperature was 150 oC and the transfer line temperature was 240 oC . The scanning mode is Selected Ion Monitoring SIM.</p>falseEffect of transplantation of irradiated mouse feces on blood short chain fatty acids in recipient miceIn order to study the effect of transplantation of irradiated mouse feces on the short chain fatty acids in the blood of recipient mice, we used mobile phones to transplant the feces of donor mice from 1W to 4W after irradiation. After 2W of antibiotic intervention, fecal bacteria were transplanted for 2W. The serum of mice was collected after two weeks, and the content of SCFAs was detected.2026-09-282026-09-21MTBLS15752