The GC-MS raw data were imported into Masshunter quantitative software (Agilent, USA, version number: v10.0.707.0). All ion fragments were automatically identificated and integrated by using default parameters,
besides, all intergration was checked manually. The metabolite concerntration of sample was calculated according to linear regression standard curve.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Gas Chromatography MS - alternating - high-polarity"],"chromatography_protocol":["25 mg solid sample were accurately weighed into 2 mL grinding tube and add 500 µL water containing 0.5%phosphoric acid. The sample were froze and ground at 50 Hz for 3 min, repeated twice, followed by ultrasonic for 10 min and centrifugation at 4 oC and 13000 g for 15 min. Take 200 µL out the supernatant aqueous solution into a 1.5 mL centrifuge tube, then add 0.2 mL N-butanol solvent containing internal standard 2-ethylbutyric acid (10 μg/mL) for exaction. Finally,vortexl for 10 s, ultrasound at low temperature for 10 min, followed by centrifugation at 4 oC and 13000 g for 5 min and the supernatant were carefully transferred to sample vials for analysis.
"],"publication":["Effect of probiotics intervention on short chain fatty acids in irradiated mice."],"submitter_affiliation":["Fourth Military Medical University: Air Force Medical University"],"submitter_name":["Guiqiang Zhou"],"organism_part":["serum","brain","feces"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Collect mouse feces, collect blood from the apex of the heart, and collect serum at 4 ℃ and 12000rpm/min. Extract mouse brain tissue.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15754"],"author":["Guiqiang Zhou. Fourth Military Medical University: Air Force Medical University. zgqiang0308@163.com."],"data_transformation_protocol":["The analysis was performed using an Agilent 8890B gas chromatography coupled to an Agilent 5977B/7000D mass selective detector with an inert electron impact (EI) ionization source and ionization voltage was 70eV (Agilent, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). Analyte compounds were separated with a HP-FFAP (30 m×0.25mm×0.25 µm) capillary column, using 99.999% helium as a carrier gas at a constant flow rate (1 mL/ min). The GC column temperature was programmed to hold at 80 oC and rise to 120 oC at a rate of 40 oC per minute, then rise to 180 oC at a rate of 10 oC per minute, finally rise to 200 oC at a rate of 20 oC per minute hold at temperature of 220 oC for 3 min. The injection volume of samples was 1 µL and introduced in splitting mode (10:1) with the inlet temperature of 230 oC. The ion sources temperature was 250 oC and the quadrupole temperature temperature was 150 oC and the transfer line temperature was 240 oC . The scanning mode is Selected Ion Monitoring SIM.
"],"study_factor":["Probiotic","Radiation"],"submitter_email":["zgqiang0308@163.com"],"sample_collection_protocol":["8 weeks after exposure to ionizing radiation and 1 week of probiotic intervention
"],"omics_type":["Metabolomics"],"study_design":["short-chain fatty acid","Metabolomics","ProteoWizard msconvert","Mus musculus","targeted analysis","8890B","Brain-Gut Axis","targeted metabolite profiling","brain","experimental sample","radiation","Agilent 5977B MSD","Anxiety","serum","feces"],"curator_keywords":["short-chain fatty acid","Metabolomics","ProteoWizard msconvert","Mus musculus","targeted analysis","8890B","Brain-Gut Axis","targeted metabolite profiling","brain","experimental sample","radiation","Agilent 5977B MSD","Anxiety","serum","feces"],"mass_spectrometry_protocol":["The analysis was performed using an Agilent 8890B gas chromatography coupled to an Agilent 5977B/7000D mass selective detector with an inert electron impact (EI) ionization source and ionization voltage was 70eV (Agilent, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). Analyte compounds were separated with a HP-FFAP (30 m×0.25mm×0.25 µm) capillary column, using 99.999% helium as a carrier gas at a constant flow rate (1 mL/ min). The GC column temperature was programmed to hold at 80 oC and rise to 120 oC at a rate of 40 oC per minute, then rise to 180 oC at a rate of 10 oC per minute, finally rise to 200 oC at a rate of 20 oC per minute hold at temperature of 220 oC for 3 min. The injection volume of samples was 1 µL and introduced in splitting mode (10:1) with the inlet temperature of 230 oC. The ion sources temperature was 250 oC and the quadrupole temperature temperature was 150 oC and the transfer line temperature was 240 oC . The scanning mode is Selected Ion Monitoring SIM.
"],"additional_accession":[]},"is_claimable":false,"name":"Effect of probiotics intervention on short chain fatty acids in irradiated mice","description":"In order to study the effect of probiotic intervention on short-chain fatty acids in irradiated mice, we collected feces, blood, and brain tissues from irradiated mice. The content of short-chain fatty acids was determined by High Performance Liquid Chromatography-mass spectrum. Further investigate the effects of ionizing radiation on the levels of short-chain fatty acids in the gut brain axis of mice, as well as the therapeutic effects of probiotics on radiation exposed mice.
","dates":{"publication":"2026-09-28","submission":"2026-09-21"},"accession":"MTBLS15754","cross_references":{}}