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identification was performed using the R XCMS package. The acquired MS/MS spectral data were matched against the mzCloud, Human Metabolome Database (HMDB), MassBank, LipidMaps, Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and an in-house database.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseChromatographic separation was performed on a Vanquish UHPLC system (Thermo Fisher Scientific, Waltham, MA, USA) using an ACQUITY UPLC HSS T3 reverse-phase column (2.1 × 150 mm, 1.8 µm; Waters). The mobile phases consisted of 0.1% formic acid in water and 0.1% formic acid in acetonitrile for the positive ion mode, and 5 mM ammonium formate in water and acetonitrile for the negative ion mode. The flow rate was 0.25 mL/min, the column temperature was 40 °C, and the injection volume was 2 µL.Integrated cecal microbiota and metabolome analyses reveal stage-specific microbiota-metabolite associations underlying host responses during Eimeria tenella infection in Wenchang chickens.Jiangsu Institute of Poultry ScienceLI Jianmeicecummass spectrometry assayCecal content samples were thawed on ice. Metabolites were extracted using pre-chilled methanol/acetonitrile (1:1, v/v). A constant volume of internal standard solution was added to all samples prior to extraction; however, the exact identity and concentration of the internal standard were not documented. After centrifugation, the supernatants were collected. Pooled quality control (QC) samples were prepared by pooling equal aliquots of all samples. No solvent blank or external standard information was recorded.Gallus gallushttps://www.ebi.ac.uk/metabolights/MTBLS15811Jianmei Li. Jiangsu Institute of Poultry Science. jianmeili157@sina.com.Raw data were converted to mzXML format by MSConvert (ProteoWizard v3.0.8789) and processed using the R XCMS package for peak detection, retention time correction, and alignment. Multivariate statistical analyses, including principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA), were performed using the R package ropls (version 1.6.2). Differential metabolites were screened using a two-tiered strategy: P < 0.05 and VIP > 1.0 for 2 dpi; VIP > 1.0, |log2 fold change| > 1, and FDR-adjusted P < 0.05 for 4 dpi and 7 dpi.TreatmentBreedTime pointjianmeili157@sina.comCecal content samples were collected from three chicken breeds—Wenchang chicken (WCC), recessive white feather chicken (RWFC), and white ear yellow chicken (WEYC)—at 26 days of age. For each breed, 60 birds were randomly allocated into two groups: an infected group (n = 30) and a non-infected control group (n = 30), with 5 replicate pens of 6 birds each. The infected group was orally inoculated with 1 mL PBS containing 50,000 sporulated E. tenella oocysts, while the control group received an equivalent volume of PBS. At 2, 4, and 7 days post-infection (dpi), one bird was randomly selected from each replicate pen per group per breed (n = 5 per group per time point per breed). Birds were euthanized by CO2 inhalation followed by cervical dislocation. Immediately after euthanasia, cecal contents were collected and snap-frozen in liquid nitrogen. All samples were stored at −80 °C until metabolomics analysis. A total of 90 cecal content samples (3 breeds × 2 groups × 3 time points × 5 replicates) were collected for metabolomics analysis.MetabolomicsThermo Scientific Vanquish UHPLC Systempooled quality control sampleMetabolomicsinfectionuntargeted analysisThermo Scientific Q Exactive HF-XTime SeriescecumGallus gallusexperimental sampleThermo Scientific Vanquish UHPLC Systempooled quality control sampleMetabolomicsinfectionuntargeted analysisThermo Scientific Q Exactive HF-XTime SeriescecumGallus gallusexperimental sampleMass spectrometry detection was performed on a Q Exactive HFX Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an electrospray ionization (ESI) source. Data were acquired in alternating positive and negative ionization modes over an m/z range of 100–1000. The key parameters were set as follows: spray voltage, +3.50 kV for the positive mode and -2.50 kV for the negative mode; sheath gas pressure, 30 arb; auxiliary gas flow, 10 arb; capillary temperature, 325 °C. The exact resolution setting for full MS scans was not recorded in the original laboratory records.Schaftoside(2S,5S)-trans-Carboxymethylproline1-Hexadecylthio-2-hexadecanoylamino-1,2-dideoxy-sn-glycero-3-phosphocholineCorticosteroneProstaglandin G2DeoxyriboseLipoxin B4RiboflavinBufadienolideAlantolactoneCephalosporin CAspartame11-O-Demethyl-17-O-deacetylvindolineImidazolelactic acidgamma-Aminobutyric acidEctoineN-a-AcetylcitrullineAminoglutethimideN1,N12-DiacetylspermineJuvenile hormone IIIKinetinL-HistidineKetoleucineVulgaxanthin I2-Keto-6-acetamidocaproateReserpineCholest-4-en-3-oneNicotinate D-ribonucleosideBesonprodilL-2-Amino-6-oxoheptanedioateO-Succinyl-L-homoserineD-Gulono-1,4-lactoneBiotinL-HomophenylalanineBz-Arg-OEt4,4'-Dihydroxy-alpha-methylstilbeneQuadroneL-Proline1-MethylxanthineBenzoate(R)-mandelic AcidMethylimidazole acetaldehydeN1-AcetylspermidineUrsolic acid3-Hydroxyanthranilic acidN-Acetyl-D-tryptophan5-Hydroxyindoleacetic acidNeocembreneGlucosamineTriacetate lactone5-Nitro-2-(3-phenylpropylamino)benzoic acidHydroxykynurenine9-cis-Retinoic acidProstaglandin E2alpha-TocopherolPilocarpine1,2,3-TrihydroxybenzeneN2-Succinyl-L-ornithinecis-4-Hydroxy-D-prolineUreidopropionic acidJasmoneTetrahydrodipicolinate6beta-HydroxytestosteroneOleic acid9,10,13-TriHOME13S-hydroxyoctadecadienoic acidTaurohyocholateLacto-N-biose I(R)-PantolactoneAbscisic alcoholAflatoxin G2Propafenone(2S)-LiquiritigeninL-beta-PhenylalanineIsoalantolactoneN-Acetyl-L-glutamate 5-semialdehydePregnanediolGlutathione12,13-DHOME7-Dehydrodesmosterol5-HydroxylysineAllopregnanoloneIsophoroneTemazepam7,8-DiaminononanoatePerillyl alcoholL-FormylkynurenineIsoetharineLinatineMethylselenopyruvateDihydrocapsaicin4-Acetamidobenzoic acid3-(2-Hydroxyphenyl)propanoic acid4-Amino-5-hydroxymethyl-2-methylpyrimidine17beta-Acetamidoandrost-4-en-3-one(R)-LaudanidineVitamin D3GuanosineSinapyl alcoholQuillaic acidAscorbateErucic acidDelta-12-Prostaglandin J2Indoleacetaldehyde8-Amino-7-oxononanoategamma-GlutamyltyraminePyridoxamine 5'-phosphatetrans-Isoasarone2-Biphenylol21-Hydroxypregnenolone4-Acetamido-2-aminobutanoic acidBiliverdinAminoadipic acidN,N-DimethylsphingosineApioleMupirocinProstaglandin D34-MethoxybenzaldehydeFMLP6'-Oxolividamine7a,12a-Dihydroxy-5b-cholestan-3-oneHecogenintrans-trans-Muconic acid(R) 2,3-Dihydroxy-3-methylvalerateCis-zeatin6-Tuliposide ASoyasapogenol B 3-O-D-glucuronideFenuronRetrorsine1-OleoylglycerophosphocholineAndrostenedioneOrnithine4-Hydroxyphenylacetaldehyde(R)-10-HydroxystearateIndoleEquolLovastatinMethylprednisolone acetate4-MethylbenzaldehydeDiazinon4-(beta-D-Glucosyloxy)benzoategamma-L-Glutamyl-D-alanineIsocitric acid6-Keto-prostaglandin F1a3-Indoleacetonitrile(5-L-Glutamyl)-L-glutamateL-Carnitine3alpha,7alpha-Dihydroxy-12-oxo-5beta-cholanateEmetineCeruleninSwainsonineThiamineCyclopentolateDiethylpropionD-LysopineAdenosineNitrendipineL-Glutamic gamma-semialdehydeAcetamidopropanalAcetylphosphate5-AcetamidovalerateProstaglandin B23-Dehydroshikimate2-Hydroxycinnamic acidLinoleic acidCycloheximideCarfentanilSulfamerazineCampesterolNornicotineL-DopaAflatoxin B13-Methyl-L-tyrosineArgininosuccinic acidMethyleugenol2,3-Dinor-8-iso prostaglandin F2alphaBetamethasoneIsopentenyl adenosineHypotaurineL-PhenylalanineStearidonic acid5-Methylcytosine9-Riburonosyladenine8,9-EETHC-toxinN-Butyryl-L-homoserine lactoneDihydrobiochanin AIsoproterenolClomipramineN-Acetyl-D-galactosamineMetoclopramide(9Z,12Z)-(8R)-Hydroxyoctadeca-9,12-dienoic acidOleanolic acidMorphinoneButein4-(Glutamylamino) butanoateIndole-3-acetaldehyde oximeEcgonineOphthalmateSpermidineCortexolone3-DehydrosphinganineSphingosineTriethylaminebeta-Zearalenol2-Naphthylamine2,5-DioxopentanoateFolinic acidNicotinamide riboside5(S)-HpETETyrosine methylester2-Isopropyl-3-oxosuccinateDehydroepiandrosterone sulfateAcetaminophenL-Cystathionine5-DehydroavenasterolCyclobenzaprineN(omega)-Hydroxyargininealpha-SpinasterolPhenylacetaldehydeMetanephrineN(6)-MethyllysineGeranyl acetateCyclopeptinePaspalicineN-DesmethylcitalopramMethostenol1-O-Vanilloyl-beta-D-glucoseN-DemethylindolmycinPyridoxal 5'-phosphateBufotalin3-MethoxyanthranilateGuanineColeonol5'-Methylthioadenosine(S)-4-HydroxymandelateOctadecanamide8,11,14-Eicosatrienoic acid1-LinoleoylglycerophosphocholineRivastigminegamma-Amino-gamma-cyanobutanoateTropateN1,N8-Bis(4-coumaroyl)spermidine8-Isoprostanem-CresolAlprenololbeta-HydroxyarginineCreatinineRiboflavin reducedMethyl hexadecanoic acidPyridoxine(2S,4S)-4-Hydroxy-2,3,4,5-tetrahydrodipicolinateMyriocinN-AcetylarylamineRetinolEmodin7-MethylguanineD-synephrineFormiminoglutamic acidOxethazaineNorcodeine2-Phenylacetamide11,12,15-THETAN-GlucosylnicotinateAtrazineThiabendazoleAcetylcholine chloride(+)-7-Isojasmonic acidQuinestrolEtiocholanedioneIsohelenolCapsaicin2-DeoxystreptamineCortisolBilirubinNicotinamide ribotideGlycocholic acid24-MethylenecycloartanolPipecolic acidNopaline9(S)-HPOTMyristicinMirtazapine7-Dehydrocholesterol1-Hydroxy-2-naphthoate(13E)-11a-Hydroxy-9,15-dioxoprost-13-enoic acidN-MethylhydantoinN6-Acetyl-N6-hydroxy-L-lysineGlutethimideHesperetinBeta-Tyrosinebeta-Alanyl-L-arginine1,3-Dihydro-(2H)-indol-2-one4-PhenylbutyrateL-Methionine S-oxide6-Amino-6-deoxyfutalosineL-2,4-diaminobutyric acidCytidineAnandamideBisphenol ADyphyllineN-Acetyl-L-aspartic acidN2-Malonyl-D-tryptophanN-Methylserotoningamma-Glutamylalanine4-Fluoro-L-threonine4-Oxoglutaramate3-Methoxy-4-hydroxyphenylglycolaldehyde3-Dehydro-2-deoxyecdysoneIsoquinolineZolpidem5-Guanidino-3-methyl-2-oxopentanoateMesobilirubinogenPyridostigmineL-Lysine(2R,3R)-3-Methylglutamyl-5-semialdehyde-N6-lysineGamabufogeninAndrostanedioneBeta-CarbolineAsiatic acid14-Dihydroxycornestin2,4-DihydroxypteridineProstaglandin I2CafestolSodium deoxycholateDioxacarb7-Methyluric acidThymolEugenolMisoprostolDodecanoic acidandrost-5-ene-3,17-dionealpha-D-Galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine2-Amino-2-deoxy-D-gluconateThyrotropin releasing hormone2-Hydroxy-3-oxoadipate(+)-SesaminSphinganinePhosphohydroxypyruvic acid2-Hydroxyestrone4-Amino-4-deoxychorismateCadaverineButyryl-L-carnitine3-KetosphingosineTraumatic AcidThymineDobutamineDocosatetraenoyl EthanolamideOleoylethanolamideTigogenin4,5-Dihydroorotic acid6'-Oxogentamicin X21-MethyladenosineQing Hau SauPirimiphos-ethylD-RiboseMaltolN-CarbamoylputrescineProgesterone(4Z,7Z,10Z,13Z,16Z,19Z)-Docosahexaenoic acid ethyl esterPyrimidodiazepinebeta-Alanyl-L-lysine1-MethylhistamineTaurineNaringenin9,10-EOTGenistinL-Alanyl-gamma-D-glutamyl-L-lysine2-Methoxy-17beta-estradiolO-PhosphoethanolamineFormylanthranilic acid1-(3,4-Dihydroxyphenyl)-5-hydroxy-3-decanoneN-Methyl-2-pyrrolidinoneLithocholic acid(R)-2-O-SulfolactateMegestrol acetate3-DehydroecdysoneD-Ribose 5-phosphate(R,S)-Coclaurine4'-OxonebramineL-Threonine2-Aminophenolp-SynephrineGlutathionylspermidine4a-MethylzymosterolAgmatineMyristoleic acidL-LeucineTrigonellinebeta-Carotenegamma-Glutamyl-beta-aminopropiononitrile(10S)-Juvenile hormone III diolPalmitoylethanolamide(R)-4-HydroxymandelateCytisine3''-Deamino-3''-oxonicotianaminePrednisoneCatecholClostebolRetinoyl b-glucuronideLeukotriene E41-palmitoylglycerophosphocholineN(omega)-Nitro-L-arginine methyl esterPorphobilinogenGlutamylglutamic acidTyrosolNalbuphine(3S,4S)-3-Hydroxytetradecane-1,3,4-tricarboxylatePentoxifyllineCortisol 21-acetateAntibiotic JI-20BAntibiotic JI-20AUbiquinone-113-L-Hydroperoxylinoleic acidDopaquinoneUndecanoic acidGabapentinCalcitriolPhenelzineSinapoyl aldehydeEthyl icosapentateFenfluraminePyrrole-2-carboxylic acidImidazole-4-acetateCarnosinePrilocaine(R)-Methylmalonate semialdehydeNeostigmineDemethylated antipyrineLincomycinQuinaldic acidSe-MethylselenocysteineXanthoxic acid2-Keto-glutaramic acidTaraxerolMethoxamineCyclizineTetracaineBenzoylagmatineSotalolNiacinamideTentoxinDiaminopimelic acidDopamineSerotoninDeoxyuridineBrevianamide F(S)-1-Phenylethanol4-Guanidinobutanoic acidCholesterolEpiandrosteroneZeranolSoyasapogenol ASoyasapogenol BFlutamidePentaporphyrin IGitogeninAnhydroglycinolRacemethionine5,6-Dihydro-5-fluorouracil8-Hydroxyquinoline2-DehydropantoateNicotianamineIndolelactic acidYamogeninTryptaminemeso-2,6-DiaminoheptanedioateN-Acetylserotonin8-HETE(+)-DemethoxyaschantinL-KynurenineRopivacaine10-Nitrolinoleic acidcis-9,10-Epoxystearic acidHarmineHellebrigenin5-Aminolevulinic acidPhytosphingosine2-MethylserineCytosinebeta-ZearalanolFisetinXanthoxinMibefradilLevamisoleErgocalciferol2-Acetamidofluorene17a-EstradiolCoumarinSpermineN-Acetyl-beta-glucosaminylamine2-Dehydro-3-deoxy-L-rhamnonate7-Oxodeoxycholate2-Phenylethanol17-HYDROXYPROGESTERONE2-Hydroxy-3-(4-hydroxyphenyl)propenoic acidPalmitoyl ethanolamideL-ArginineNevirapineEthirimolEnterolactone4-Hydroxy-2-quinoloneepsilon-(gamma-L-Glutamyl)-L-lysineN8-AcetylspermidineIndole-3-acetateGalactosylsphingosineLenacil1,1-DimethylbiguanideN5-(L-1-Carboxyethyl)-L-ornithineNicotinic acid mononucleotideN-Succinyl-L,L-2,6-diaminopimelate2-Hydroxybutyric acidQuinolinic acidbeta-D-3-Ribofuranosyluric acid(S)-N-Methylcoclaurinep-OctopamineMalonobenHomocitrullineDiphenhydramineCholine sulfate5-KETEGamma-Tocotrienol2-HydroxyphenethylaminedAMPAllocystathionineCortisone9(S)-HOTCinchonidineCitraconic acidRepaglinideInosinePurineS-Methyl-L-methionineNorepinephrineLumichromeDimethylglycineL-Histidine trimethylbetaine1D-1-Guanidino-3-amino-1,3-dideoxy-scyllo-inositolLevonordefrinMethylimidazoleacetic acid(R)-3-UreidoisobutyrateN-Acetyl-L-phenylalanine(3S)-3,6-Diaminohexanoate16-Feruloyloxypalmitate9,10-DihydroxystearateArachidonic acidCarnosolChitobiose4a-Carbinolamine tetrahydrobiopterinSubaphyllineIlludin M7,8-Dihydropteroic acid(S)-NorlaudanosolineSaccharopineDiethylstilbestrolConiferyl aldehydetrans-1,2-Dihydrobenzene-1,2-diolTyramineResolvin D2(S)-5-Amino-3-oxohexanoate3-Epiecdysone(3S)-6-Acetamido-3-aminohexanoateAllocholic acidTetrahydropteridine(S)-3-Hydroxy-N-methylcoclaurine4-Hydroxybenzoic acid5,6-DHETL-MethionineDillapiolDihydrouracilMelezitose(R)-5,6-DihydrothymineCiliatineQuercetinAntipyrineN-AcetylhistamineMethyldopa24,25-DihydrolanosterolGlycerophosphocholineTheophylline13(S)-HpOTrEErgothioneine1-Aminocyclopropanecarboxylic acidEthynodiol Diacetate2,3-Dinor-8-iso prostaglandin F1alphaCapecitabineNalidixic AcidN-Acetylornithine5-Amino-2-oxopentanoic acidPropazine6-HydroxymelatoninNeocnidilide17a-HydroxypregnenolonePicolinic acidAncymidolAspartylglycosamineApigeninPrephenateN7-MethylguanosineCinobufotalin3-Amino-4-hydroxybenzoateEcdysonePyridoxamine8,9-DiHETrE4-HydroxycinnamoylagmatineN-Acetylhistidine3,4-MethylenedioxyamphetamineOrciprenaline(S)-Reticuline2-OxoarginineN-AcetylputrescineAurachin BO-AcetylcarnitineMannitol 1-phosphateAndrosteroneTocainideS-AdenosylmethionineGlycyrrhetinate4a-Carboxy-4b-methyl-5a-cholesta-8,24-dien-3b-olProtriptylineImperatorinGentamicin X2L-Pyrrolysine3-(3,4-Dihydroxyphenyl)pyruvate2-Hydroxy-6-pentadecylbenzoic acidAcetylcholineL-4-HydroxyphenylglycineL-Lysine 1,6-lactamKhellinMethaqualoneCapsidiolIsochavicolKyotorphinN-AcetylmuramateAcetylcysteineMethyl isobutyl ketoneHomatropine26-HydroxyecdysoneNandroloneBaclofenAsymmetric dimethylarginineTryptophanolD-Octopine(24R,24(1)R)-Fucosterol epoxide8-HydroxyguanineDocosanedioate5,7-DihydroxyisoflavoneDulcin11,14,15-THETABetulin5-Oxo-1,2-campholide18-HydroxycorticosteroneBenzaldehyde(2S)-Flavanone1-Pyrroline-2-carboxylic acidPhosphorylcholineMethyl (indol-3-yl)acetateExemestane3-MethylindoleBufalinMethyl jasmonateConfertifolinIminoarginineQuinidinePalmitoyl-L-carnitineL-Aspartic acidProcaineDyclonineEthyl oleateN(6)-[(Indol-3-yl)acetyl]-L-lysine2'-DeoxyadenosineSarsasapogeninN-Alpha-acetyllysine3,3',4'5-TetrahydroxystilbeneL-Targinine(2R,3R)-3-Methylornithinyl-N6-lysineAll-trans-hexaprenyl diphosphateN-Acetyl-L-2-amino-6-oxopimelateOxycodone2-Deoxy-scyllo-inososeLacto-N-tetraoseLeucocyanidinOxazepamRibavirinCitalopramN2-Succinyl-L-arginineSenecionineO-Acetyl-L-homoserineBitertanolDihydrothymineParthenin2,3-ButanediolMedroxyprogesterone2-Aminobenzoic acidL-2-Hydroxyglutaric acidp-Aminobenzoic acid(R)-b-aminoisobutyric acidL-4-Hydroxyphenylglycyl-L-arginine5alpha-Ergosta-7,22-diene-3beta,5-diol11b-Hydroxyandrost-4-ene-3,17-dioneEpinephrineMetaxaloneN-Methyl-L-glutamic acidDethiobiotinClozapineRutinDeoxycholic acidN1-AcetylspermineAnisomycinEstroneMestranol(2R,5S)-2,5-DiaminohexanoatefalseUHPLC-MS/MS Untargeted Metabolomics Profiling of Cecal Content in Three Chicken BreedsThis study applied an untargeted metabolomics approach to analyze cecal content samples from three chicken breeds (Wenchang chicken, White-ear Yellow chicken, and Recessive White chicken) using UHPLC-MS/MS (Vanquish UHPLC coupled with Q Exactive HFX Orbitrap). A total of 90 samples (30 per breed) plus 8 pooled QC samples were analyzed. Metabolites were extracted with methanol/acetonitrile (1:1, v/v), and data were acquired in positive and negative ionization modes. Raw data were converted to mzXML format by MSConvert (ProteoWizard v3.0.8789) and processed using the R XCMS package for peak detection, retention time correction, and alignment. Metabolites were annotated against HMDB, MassBank, LipidMaps, mzCloud, KEGG, and an in-house database. Data normalization was performed using QC-RLSC (LOESS signal correction), and features with QC RSD > 30% were filtered out. Multivariate statistics (PCA and OPLS-DA) were conducted using the R package ropls to distinguish metabolic profiles. Differential metabolites were screened using a two-tiered strategy: for 2 dpi, P < 0.05 and VIP > 1.0 were used; for 4 dpi and 7 dpi, VIP > 1.0, |log2FC| > 1, and FDR-adjusted P < 0.05 were applied. Pathway enrichment analysis was performed using MetaboAnalyst; no pathway reached P < 0.05, and pathways with P < 0.2 were considered as trends.Breed name mapping: In the commercial dataset folders, breeds are abbreviated as WCJ (Wenchang chicken), BEJ (White-ear Yellow chicken), and YXB (Recessive White chicken). These correspond to WCC, WEYC, and RWFC, respectively, as used in this study and the manuscript.Note: The associated manuscript analyzes and reports conclusions based exclusively on the Wenchang chicken breed; data from the other two breeds (YXB and BEJ) are included for project-level transparency. They have not been used in the analyses presented in this manuscript.Data source clarification: The manuscript analyses are based on the 2023 commercial metabolomics report, which was generated from the complete original raw data at that time. The raw data recovered in 2026 are deposited in MetaboLights (MTBLS15811) and in the Zenodo record for archival and transparency purposes. A small number of recovered files have reduced scan counts due to the original corruption; however, all files can be opened, and this does not affect the manuscript conclusions, which rely on the 2023 commercial report.2026-10-082026-09-25MTBLS15811C04546C01616C05844C03872C05931C02895C03277C16583C01225C00826C05620C05399C00417C12208C20383C21124C00977C00331C04290C03492C00522C00831C01133C00881C12124C19787C00855C01180C05619C15970C19614C01192C21656C05526C16597C06153C00515C00085C01134C04079C20328C04707C04164C00208C02655C01175C05143C16827C05403C04661C11500C04359C05382C16688C18096C00689C03921C12205C04352C02130C20861C03742C13455C15586C01019C00490C16536C16522C16534C16533C01421C10723C10199C16320C16316C03935C14833C14836C14750C07506C06313C08314C00817C08367C08365C10202C14809C00258C00106C00489C01384C00122C00431C00147C00581C00183C00719C02170C00042C00503C05332C06730C14790C06423C00026C00064C01879C00407C00300C07086C00548C00628C00262C00805C00785C00587C00322C03114C00025C06672C00544C00181C00379C03663C00230C00196C08278C03139C01601C00745C01197C00124C00137C00082C00507C00166C00811C16357C02265C01606C00366C08261C01717C00624C06414C02710C00327C03619C00257C00198C00159C00247C13747C00095C00794C00392C02470C01924C00158C00879C01494C00191C17076C10833C08317C00864C00140C00645C00078C00525C01990C00643C01262C00214C00299C06424C00669C10028C00491C00823C00330C00249C00345C01530C01514C06563C01227C06562C09727C06427C10098C05903C00239C16535C00777C06425C02165C00055C01699C00639C00354C00185C01780C14554C02528C01724C05466C01460C01714C16939C05122C14641C00232C00109C01089C00065C00530C02045C00253C07446C06104C00149C01620C07215C01020C03576C05608C02137C03005C00385C05852C05593C00606C05565C01551C00993C19837C06337C01161C01152C01424C05576C02693C03145C07588C10726C01586C10906C06662C10447C03672C16511C05580C08277C01005C02727C02076C08491C17497C08042C05584C12147C05824C00482C00251C02305C00199C02678C11437C06997C00620C07020C01657C05512C07443C01716C16537C06082C02267C10208C06186C11110C10494C14536C06991C18218C14204C00774C09952C04056C01762C18166C06900C12270C05301C19909C14827C14766C06939C14825C10084C14717C12508C10038C14778C16525C16526C14774C14748C00364C09264C00105C05490C06429C11720C16513C16527C07508C08357C05957C05953C16417C08427C01367C05950C06314C06476C00427C05959C06438C06475C06124C08323C05962C05964C05963C13707C09226C07589C06875C04036C06843C14153C07469C07398C00353C08325C00440C06624C18043C01835C07373C08794C02191C00016C03030C10023C01904C12136C01697C09644C20380C13782C02372C00388C00036C06103C00263C00815C03465C06608C08493C07326C02979C00493C01047C19180C05380C05582C00847C02155C08255C02029C06821C06536C09382C14302C08362C11169C05283C03882C06561C14214C13698C10719C10521C10462C06428C14828C10040C08281C00696C10682C18167C08778C10545C10706C05951C06834C10195C01732C00497C02656C02946C14200C05607C07648C01179C02496C00296C00806C09780C03592C16765C00092C00858C05300C19615C09281C05855C18502C10770C05623C19242C15767C05841C03003C05730C02837C02728C04211C20926C00788C05831C20155C05513C04421C03771C18473C01682C20910C02993C02666C02700C04555C00674C00486C05827C02253C18170C11512C18217C14769C05715C04221C02937C05298C05906C01921C04643C01292C21029C04529C02916C20470C03087C03871C02325C16138C20278C05202C19763C21267C20487C03414C00919C05176C20252C03772C01230C05453C00439C10449C08483C08318C09485C14776C13802C14782C01670C04100C10364C07251C14835C14814C14752C14751C14753C08914C08899C17419C16355C17844C07620C20577C16324C16326C19625C14795C07618C06371C16921C16932C05111C03916C08972C17705C17702C15149C16962C05103C16497C16923C16969C14691C01234C01672C00334C01026C11348C01467C00519C01987C05942C00380C00233C00429C00148C00188C02735C06044C00601C10164C00245C03557C00178C00408C01012C06524C00073C03440C00123C00049C00121C00568C00483C00156C00108C00346C00956C01046C00955C00318C00315C01035C00047C03196C00547C00314C02452C00632C00265C12115C06325C00135C02938C00337C00072C00398C06809C03212C00079C03722C12323C03203C12989C02637C20635C00666C05635C00062C05410C03752C07130C08201C02679C17715C03519C00328C00355C01909C00978C00371C00750C01239C02794C00386C00526C00117C01727C14131C00475C00931C00018C00212C00294C00559C02537C09762C01595C02494C00712C00387C00170C00836C10041C03242C00523C07635C06800C01709C00389C05484C00051C00360C00455C05488C08316C00762C03958C02140C00255C03990C05443C00187C04483C05441C00019C02821C11171C02477C09126C02094C00500C05625C02226C00791C01801C15587C01108C00430C02642C12312C01983C10761C00637C01996C01198C06804C00437C04368C01772C10453C16358C04548C13008C17002C09861C02997C05588C07410C11768C03626C02043C06954C00612C06212C07513C07144C07489C07934C12058C07258C08322C07056C05570C03740C07053C00542C00647C05643C07560C09484C09376C06960C05901C00378C07570C06930C16995C06931C16300C07424C09269C07309C07881C00509C06932C11379C08162C14762C07868C19039C09538C15493C05302C03413C14324C14497C06866C16608C04717C06918C00410C03425C02934C00319C16512C00219C06527C13914C09069C05138C14732C11258C14772C16184C10759C05485C13809C14773C07370C12650C01124C11695C05961C04654C01789C01164C14155C03963C01673C11596C05952C08618C07670C03479C04102C11758C06539C01004C00329C11766C06848C14743C01733C00311C13624C00280C10343C09066C06176C05790C00090C00077C01042C01118C01132C02291C01477C00735C07142C07263C07254C07653C13846C07359C00153C02376C06323C00906C02505C07565C16741C09908C05135C10858C20157C02427C03404C13244C05341C06551C07531C07375C00644C00468C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