Hierarchical clustering heatmaps were generated in R using the ComplexHeatmap package
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - hilic"],"chromatography_protocol":["Chromatographic separation was performed using an Agilent 1290 Infinity LC system equipped with an XBridge Amide column (3.5 µm, 4.6 × 150 mm; Waters) using hydrophilic interaction liquid chromatography (HILIC).
"],"publication":["DEGS1-mTORC1-driven lipid metabolic reprogramming fuels rhabdomyosarcoma progression and unveils a therapeutic vulnerability."],"submitter_affiliation":["Duke-NUS Medical School"],"submitter_name":["Priyadarshini Gopal"],"organism_part":["cell"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["lysate was mixed with 270 µL of ice-cold acetonitrile, followed by vortexing and/or additional sonication to ensure homogeneity. Samples were aliquoted as required for downstream analyses. 25uL of samples were added with 25 μL of the premixed lipid internal standard (Sciex lipidyzer platform) extracted in a DCM: Methanol: H20 mixture. Evaporate solvent and reconstitute the extracted lipids in 100 μL of MeOH.
"],"organism":["Mus musculus","Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15896"],"author":["Hong Wen Tang. Duke-NUS Medical School. hongwen.tang@duke-nus.edu.sg.","Priyadarshini Gopal. Duke-NUS Medical School. priya.gk@duke-nus.edu.sg."],"data_transformation_protocol":["Data were log2-transformed and row-wise z-scored prior to heatmap generation.
"],"study_factor":["Cell line","Genetic modification"],"submitter_email":["priya.gk@duke-nus.edu.sg"],"sample_collection_protocol":["Cells were cultured to full confluency in standard tissue culture plates. Cells were washed once with 2 mL of ice-cold phosphate-buffered saline (PBS), then incubated with an additional 1 mL of ice-cold PBS and scraped from the plate. After centrifugation at 1,000 x g for 5 minutes at 4 °C, the supernatant was aspirated, and cell pellets were resuspended in 300 µL of ice-cold deionized water containing 0.6% formic acid. Samples were sonicated on ice for 10 minutes to ensure complete lysis.
"],"omics_type":["Metabolomics"],"study_design":["Mus musculus","targeted analysis","Homo sapiens","Lipidomics","AB SCIEX QTRAP 5500 System","cell","rhabdomyosarcoma","Agilent 1290 Infinity LC","DUKE- NUS Metabolomics Facility"],"curator_keywords":["Mus musculus","targeted analysis","Homo sapiens","Lipidomics","AB SCIEX QTRAP 5500 System","cell","rhabdomyosarcoma","Agilent 1290 Infinity LC","DUKE- NUS Metabolomics Facility"],"mass_spectrometry_protocol":["Chromatographic separation was achieved using XBridge Amide 3.5 μm, 4.6 × 150 mm column (Waters). Positive ion mode was used to detect SM/CER/DCER/HCER/LCER/TAG/DAG/MAG, while negative ion mode was used to detect PC/PE/PG/PI/PS/FFA.Raw LC–MS data were processed using MultiQuant software (SCIEX). Lipid species were annotated based on parent and daughter fragments and retention time. To check for the quality of data, PCA plots for the samples, blank and internal standard controls were generated using Agilent Mass Profiler Professional (MPP) software.
"],"additional_accession":[]},"is_claimable":false,"name":"DEGS1-mTORC1-driven lipid metabolic reprogramming fuels rhabdomyosarcoma progression and unveils a therapeutic vulnerability","description":"These findings establish a mechanistic link between sphingolipid metabolism, mTORC1-mediated regulation of lipophagy, and tumor aggressiveness, highlighting lipid droplet metabolism as a novel and tractable therapeutic target in RMS.","dates":{"publication":"2026-10-02","submission":"2026-10-02"},"accession":"MTBLS15896","cross_references":{}}