MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15898/m_MTBLS15898_NMR___v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15898/FILES/DERIVED_FILES/Univariate_statistical_outputs.xlsxftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15898/s_MTBLS15898.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15898/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15898/a_MTBLS15898_NMR__.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15898/FILES/RAW_FILES/nmr_raw_data.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15898<p>Metabolites were assigned by querying metabolome databases, such as the Human Metabolome Database and the MadisonQingdao Metabolomics Consortium Database, aided by the Chenomx NMR suite 8.1 software (Chenomx Inc., Edmonton, Canada) and the statistical total correlation spectroscopy (STOCSY) technique.</p>MetaboLightsPublicNuclear Magnetic Resonance (NMR) -Integrated pharmacological and metabolomic characterization of a polysaccharide-enriched fraction from Qingfei-Paidu Decoction for protection against acute lung injury in mice.<p>NMR spectra were recorded on a Bruker AVANCE III 500 MHz NMR spectrometer at 298 K. D2O was used for field frequency locking and TSP was used as a chemical shift reference (1H, 0.00 ppm). A cpmgpr1d sequence was used to suppress the signals of the proteins. 1H NMR spectra were measured with 64 scans into 32 K data points over a spectral width of 7500 Hz.</p>Anhui Normal UniversityMinghui Liblood serumNMR spectroscopy assay<p>Briefly, 300 μL serum samples were mixed with methanol in 1 : 2 ratios (v/v), vortexed and incubated at -20 ℃ for 20 min. The mixtures were centrifuged into pellet proteins at 13 400 rpm for 30 min. The obtained supernatants were decanted into fresh vials and dried. </p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS15898Minghui Li. Anhui Normal University. liminghui@ahnu.edu.cn.<p>The spectra were Fourier transformed by multiplication of the FIDs (free induction decay) with an exponential weighting function corresponding to a line-broadening of 0.5 Hz by using the TopSpin software.</p>Ctrl, LPS, etoh, PSCliminghui@ahnu.edu.cn<p>Blood samples were collected, coagulated at room temperature for 30 min and then centrifuged at 3000 rpm, 4 ℃ for 20 min to harvest serums.</p>MetabolomicsHerbal medicineMetabolomicsMus musculusuntargeted analysisinflammationblood serumBruker 500 MHz spectrometeracute lung injuryexperimental sampleHerbal medicineMetabolomicsMus musculusuntargeted analysisinflammationblood serumBruker 500 MHz spectrometeracute lung injuryexperimental sample<p>The dried samples were dissolved in 600 μL 99.8% D2O phosphate buffer (0.2 M, pH at 7.0) containing 0.05% (w/v) TSP, vortexed and transferred to 5 mm NMR tubes.</p>falseIntegrated pharmacological and metabolomic characterization of a polysaccharide-enriched fraction from Qingfei-Paidu Decoction for protection against acute lung injury in miceThe formula of Qingfei-Paidu Decoction was separated into a polysaccharide-enriched fraction (PSC) and an ethanol-soluble small-molecule fraction (EtOH), whose biological activities were systematically compared under equivalent crude drug conditions using an LPS-induced ALI mouse model and macrophage inflammatory assays. PSC exhibited superior protective activity compared with EtOH, characterized by greater attenuation of pulmonary pathological injury, edema formation, and inflammatory cytokine production. Furthermore, 1H NMR-based serum metabolomics combined with shared-and-unique structures analysis revealed distinct metabolic fingerprints between the two fractions. Compared with EtOH, PSC produced broader metabolic remodeling, particularly involving energy metabolism.2026-10-052026-10-02MTBLS15898