Metabolites were identified by matching accurate mass and MS/MS fragmentation data against mzCloud, mzVault, and MassList databases. Additional metabolite annotations were performed using KEGG, HMDB, and LIPID MAPS.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Chromatographic separation was performed using a Thermo Scientific Vanquish UHPLC system equipped with a Hypersil GOLD C18 column. Gradient elution was conducted using methanol and aqueous mobile phases under positive and negative ionization conditions, with a flow rate of 0.2 mL/min and column temperature of 40 °C.
"],"publication":["Latilactobacillus sakei alleviates Helicobacter pylori gastritis via gastric microbiota remodeling and nicotinic acid-mediated reprogramming of host NAD⁺ metabolism."],"submitter_affiliation":["Shaoxing University, Shaoxing, Zhejiang, China"],"submitter_name":["Chenlan Xia"],"organism_part":["stomach"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Cellular metabolites were extracted using 80% aqueous methanol, followed by freeze-thaw treatment, sonication, centrifugation, and reconstitution for LC-MS analysis. Pooled quality control samples and solvent blanks were prepared for analytical quality assessment.
"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15966"],"author":["Chenlan Xia. Shaoxing University, Shaoxing, Zhejiang, China. xiachenlan@usx.edu.cn."],"data_transformation_protocol":["Raw LC-MS data were processed using Compound Discoverer 3.1 for peak detection, alignment, integration, and normalization. Background signals were removed using blank samples, and metabolic features with a coefficient of variation exceeding 30% in quality control samples were excluded.
"],"study_factor":["Treatment"],"submitter_email":["xiachenlan@usx.edu.cn"],"sample_collection_protocol":["Human gastric epithelial AGS cells were collected from control, L. sakei-treated, H. pylori-infected, and combined treatment groups for untargeted metabolomic analysis. Five biological samples were included in each group.
"],"omics_type":["Metabolomics"],"study_design":["Thermo Scientific Vanquish UHPLC System","Metabolomics","Helicobacter pylori","untargeted analysis","Thermo Scientific Q Exactive HF-X","Latilactobacillus sakei","Homo sapiens","stomach","gastritis","experimental sample"],"curator_keywords":["Thermo Scientific Vanquish UHPLC System","Metabolomics","Helicobacter pylori","untargeted analysis","Thermo Scientific Q Exactive HF-X","Latilactobacillus sakei","Homo sapiens","stomach","gastritis","experimental sample"],"mass_spectrometry_protocol":["Mass spectrometric analysis was performed using a Thermo Scientific Q Exactive HF-X mass spectrometer equipped with an electrospray ionization source. Data were acquired in positive and negative ion modes over an m/z range of 100–1500, with data-dependent MS/MS acquisition.
"],"additional_accession":[]},"is_claimable":false,"name":"Untargeted LC-MS/MS metabolomic profiling of Helicobacter pylori-infected gastric epithelial cells treated with Latilactobacillus sakei","description":"This study employed untargeted LC-MS/MS metabolomics to investigate the effects of Latilactobacillus sakei on metabolic alterations in Helicobacter pylori-infected gastric epithelial cells. Metabolomic analysis revealed that Latilactobacillus sakei intervention modulated host cellular metabolism, particularly nicotinate and nicotinamide metabolism, suggesting a potential role for bacterial-associated metabolites in restoring metabolic homeostasis during H. pylori infection.","dates":{"publication":"2026-10-09","submission":"2026-10-09"},"accession":"MTBLS15966","cross_references":{}}