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b/databases/metabolights/studies/public/MTBLS15966/FILES/RAW_FILES/HFX10_CN8_FZTM220082498-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15966/FILES/RAW_FILES/HFX10_CN2_FZTM220082465-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15966/FILES/RAW_FILES/HFX10_CP6_FZTM220082490-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15966/FILES/RAW_FILES/HFX10_CN8_FZTM220082499-1A.rawprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15966<p>Metabolites were identified by matching accurate mass and MS/MS fragmentation data against mzCloud, mzVault, and MassList databases. Additional metabolite annotations were performed using KEGG, HMDB, and LIPID MAPS.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using a Thermo Scientific Vanquish UHPLC system equipped with a Hypersil GOLD C18 column. Gradient elution was conducted using methanol and aqueous mobile phases under positive and negative ionization conditions, with a flow rate of 0.2 mL/min and column temperature of 40 °C.</p>Latilactobacillus sakei alleviates Helicobacter pylori gastritis via gastric microbiota remodeling and nicotinic acid-mediated reprogramming of host NAD⁺ metabolism.Shaoxing University, Shaoxing, Zhejiang, ChinaChenlan Xiastomachmass spectrometry assay<p>Cellular metabolites were extracted using 80% aqueous methanol, followed by freeze-thaw treatment, sonication, centrifugation, and reconstitution for LC-MS analysis. Pooled quality control samples and solvent blanks were prepared for analytical quality assessment.</p>Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS15966Chenlan Xia. Shaoxing University, Shaoxing, Zhejiang, China. xiachenlan@usx.edu.cn.<p>Raw LC-MS data were processed using Compound Discoverer 3.1 for peak detection, alignment, integration, and normalization. Background signals were removed using blank samples, and metabolic features with a coefficient of variation exceeding 30% in quality control samples were excluded.</p>Treatmentxiachenlan@usx.edu.cn<p>Human gastric epithelial AGS cells were collected from control, L. sakei-treated, H. pylori-infected, and combined treatment groups for untargeted metabolomic analysis. Five biological samples were included in each group.</p>MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicsHelicobacter pyloriuntargeted analysisThermo Scientific Q Exactive HF-XLatilactobacillus sakeiHomo sapiensstomachgastritisexperimental sampleThermo Scientific Vanquish UHPLC SystemMetabolomicsHelicobacter pyloriuntargeted analysisThermo Scientific Q Exactive HF-XLatilactobacillus sakeiHomo sapiensstomachgastritisexperimental sample<p>Mass spectrometric analysis was performed using a Thermo Scientific Q Exactive HF-X mass spectrometer equipped with an electrospray ionization source. Data were acquired in positive and negative ion modes over an m/z range of 100–1500, with data-dependent MS/MS acquisition.</p>falseUntargeted LC-MS/MS metabolomic profiling of Helicobacter pylori-infected gastric epithelial cells treated with Latilactobacillus sakeiThis study employed untargeted LC-MS/MS metabolomics to investigate the effects of Latilactobacillus sakei on metabolic alterations in Helicobacter pylori-infected gastric epithelial cells. Metabolomic analysis revealed that Latilactobacillus sakei intervention modulated host cellular metabolism, particularly nicotinate and nicotinamide metabolism, suggesting a potential role for bacterial-associated metabolites in restoring metabolic homeostasis during H. pylori infection.2026-10-092026-10-09MTBLS15966