Lipid Search is a search engine for the identification of lipid species based on MS/MS math. LipidSearch contains more than 30 lipid classes and more than 1,500,000 fragment ions in the database. Both mass tolerance for precursor and fragment were set to 5 ppm.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse phase","Liquid Chromatography MS - positive - reverse phase"],"chromatography_protocol":["Reverse phase chromatography was selected for LC separation using CSH C18 column (1.7 µm, 2.1 mm x 100 mm, Waters) and UHPLC Nexera LC-30A ultra performance liquid chromatography system. The lipid extracts were re-dissolved in 200 µL 90% isopropanol/acetonitrile, centrifuged at 14,000 x g for 15 min, finally 3 µL of sample was injected. Solvent A was acetonitrile:water (6:4, v/v) with 0.1% formic acid and 0.1 mM ammonium formate and solvent B was acetonitrile:isopropanol (1:9, v/v) with 0.1% formic acid and 0.1 mM ammonium formate. The initial mobile phase was 30% solvent B at a flow rate of 300 μL/min. It was held for 2 min, and then linearly increased to 100% solvent B in 23 min, followed by equilibrating at 5% solvent B for 10 min.
"],"publication":["Analysis for lipid nutrient differences in the milk of 13 species from a quantitative non-targeted lipidomics perspective. 10.1016/j.fochx.2023.101024. PMID:38144754"],"submitter_name":["Zeying Wang"],"submitter_affiliation":["Shenyang Agricultural University"],"organism_part":["milk"],"technology_type":["mass spectrometry"],"disease":[""],"extraction_protocol":["Lipids were extracted according to MTBE method. Briefly, samples were first spiked with appropriate amount of internal lipid standards and then homogenized with 200 µL water and 240 µL methanol. After that, 800 µL of MTBE was added and the mixture was ultrasound 20 min at 4 °C followed by sitting still for 30 min at room temperature. The solution was centrifuged at 14,000 x g for 15 min at 10 °C and the upper organic solvent layer was obtained and dried under nitrogen.
"],"organism":["Equus caballus","Ovis aries","Camelus","Bos grunniens","Equus asinus","Homo sapiens","Bos taurus","Bubalus","Sus scrofa","Homo sapiens;Camelus;Bos taurus;Bubalus;Bos grunniens;Equus asinus;Equus caballus;Ovis aries;Sus scrofa"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS6767"],"author":["Sun Yinggang. Shenyang Agricultural University. Shenyang Agricultural University. 1229277434@qq.com. 18842468110.","Wu Yanzhi. Shenyang Agricultural University. Shenyang Agricultural University. 765570823@qq.com. 13961979117."],"data_transformation_protocol":["Lipid Search is a search engine for the identification of lipid species based on MS/MS math. LipidSearch contains more than 30 lipid classes and more than 1,500,000 fragment ions in the database. Both mass tolerance for precursor and fragment were set to 5 ppm.
"],"study_factor":["Replicate","Milk"],"submitter_email":["wangzeying2012@syau.edu.cn"],"sample_collection_protocol":["In 2021, we collected 6 forms each species of 78 mature milk samples obtained from 13 species of healthy mammals as follows: Chinese human milk samples (CHP) were donated by Chinese mothers from Shenyang Maternal and Child Care Center. Holstein cow milk samples (HST) were obtained from Huishan Dairy Group. Buffalo milk samples (GXB) were obtained from Guangxi Buffalo Research Institute and Nanjing Agricultural University. Yak milk samples (QHY) were obtained from Qinghai province and Lanzhou institute of husbandry and pharmaceutical science of cass. DeZhou Donkey milk samples (DZD) were obtained from East Ajiao Donkey Farm in Fumeng County. Mongolian Horse milk samples (MGH) were obtained from Inner Mongolia XilinGol League Science and Technology Association. Alxa camel milk samples (ALC) were obtained from Alxa Alpaca Farm in Inner Mongolia. Saanen goat milk samples (SNG) and Toggenburg goat milk samples (TGB) and Nubian goat milk samples (NBY) were obtained from Liaoyang dairy goat farm. Liaoning Cashmere goat milk samples (LCG) were obtained from Liaoyang National Core Sheep Breeding Farm. Small Tail Han sheep milk samples (OAS) were obtained from Inner Mongolia Horinger County sheep farm. Pig milk samples (YXP) were obtained from Yangxiang Pig Group. In all species, milk samples were collected during paired lactation weeks after birth. At the end of each feeding or milking, the milk sample is removed and placed in the same 10 mL plastic container and immediately placed in a refrigerator at -20 °C for a short period of time, not exceeding 2 weeks. The samples are then transported in undefrosted dry ice and placed in a freezer at -80 °C for long-term storage, but for no more than 4 months.
Ethical statements
The Animal Welfare and Ethics Committee of Shenyang Agricultural University approved the participation of people and animals in this study. Approval No.: 20210601.
"],"omics_type":["Metabolomics"],"study_design":["ultra-performance liquid chromatography-mass spectrometry","tandem mass spectrometry","livestock","Lipidomics","untargeted metabolites","dairy"],"curator_keywords":["ultra-performance liquid chromatography-mass spectrometry","tandem mass spectrometry","livestock","Lipidomics","untargeted metabolites","dairy"],"mass_spectrometry_protocol":["Mass spectra was acquired by Q-Exactive Plus in positive and negative mode, respectively. ESI parameters were optimized and preset for all measurements as follows: Source temperature, 300 °C; Capillary Temp, 350 °C, the ion spray voltage was set at 3000 V, S-Lens RF Level was set at 50% and the scan range of the instruments was set at 200-1800 m/z.
"],"pubmed_abstract":["Lipids are essential organic components in milk and have been associated with various health benefits for newborns. However, a comprehensive analysis of lipid profiles across multiple species and levels has been lacking. In this study, we employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) to accurately determine the absolute content of lipid molecules. It revealed that ruminants exhibit a higher concentration of short-chain fatty acids compared to non-ruminants. Additionally, we identified ALC (camel), MGH (horse), and DZD (donkey) as species that display similarities to components found in human milk fat. Remarkably, it reveals that porcine milk fat is characterized by long chain lengths, low saturation, and a high proportion of essential fatty acids. PS (22:5_18:2) could potentially serve as a biomarker in porcine milk. These unique characteristics present potential opportunities for the utilization of porcine milk. Overall, our findings provide valuable insights into the lipidomics profiles of milk from different species."],"pubmed_title":["Analysis for lipid nutrient differences in the milk of 13 species from a quantitative non-targeted lipidomics perspective."],"pubmed_authors":["Wu Yanzhi Y, Sun Yinggang Y, Chen Rui R, Qiao Yanjun Y, Zhang Qiu Q, Li Qian Q, Wang Xiaowei X, Pan Yuan Y, Li Siyi S, Wang Zeying Z"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of lipid nutrient differences in the milk of 13 species from a quantitative non-targeted lipidomics perspective","description":"Lipid, an important organic component in milk, has health benefits for newborns. Profiles of milk lipid in multi-species from multi-level haven’t been accessible, therefore, we established a lipid subclass, lipid molecule, and fatty acid profile of 13 species to compare interspecific lipid differences. We used lipidomic to analysis the absolute content of lipid molecules based on UPLC-Orbitrap mass spectrometry system. The content of Zymosterol (ZyE) in all livestock was much higher than that in human milk. Meanwhile, the content of PI (18: 0_20: 5) and SM(d30:1) are only high in camels, which it might be used as potential biomarkers for the identification of camel milk. High content of ultra-long chain fatty acids (ULCFAs), as well as high content of DHA, ARA, EPA were found in pig milk. It is hoped that this comprehensive analysis of multi-species lipidomic can provide new macro insights into the development of the dairy industry.
","dates":{"publication":"2023-08-21","submission":"2022-12-21"},"accession":"MTBLS6767","cross_references":{"pubmed":["38144754"]}}