NODEOtherLeliang ZhengWGSIllumina HiSeq 3000https://www.biosino.org/node/experiment/detail/OEX00002075PairedDNAse2['bronchoalveolar Lavage']['Homo sapiens']Briefly, DNA was sheared by ultrasonication (Covaris, Woburn, MA). The sheared DNA fragments were end-repaired (DNA End Repair Mix) at 20°C for 30 min. The DNA fragments were purified by QIAquick PCR Purification Kit (Qiagen) and A-tailed using A-Tailing Mix. Libraries were checked using Bioanalyzer 2100 (Agilent) and quantified using the ABI StepOnePlus Real-Time PCR System. Libraries were sequenced on an Illumina platform. The contigs obtained after de novo assembly were applied for gene prediction using MetaGeneMark. The predicted genes were clustered using CD-hit. The reads were mapped to this combined gene pool using Bowtie 2 for quantification of genes and species.NODEfalseThe metagenomic sequencingThe metagenomic sequencing was performed by the paired-end sequencing method on the Illumina platform (BGI, China).2020-05-052019-11-01OEX00002075OEP00000655