{"database":"NODE","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"submitter":["Zhaoyuan Fang"],"technology_type":["ChIP-Seq"],"full_dataset_link":["https://www.biosino.org/node/experiment/detail/OEX00002089"],"experiment_platform":["Illumina HiSeq 2000"],"experiment_library_layout":["Paired"],"experiment_library_selection":["ChIP"],"experiment_mate_pair":["Y"],"sample_count":["4"],"tissue":["['lung']"],"taxonomy":["['Mus musculus']"],"experiment_protocol":["Cells were cross-linked with 1% formaldehyde for 5 min at room temperature, lysed by SDS Lysis buffer and sonicated to generate DNA fragments with an average size of 500 bp. After pre-clearing with Protein G beads, antibodies against ARID2 (SC 98299X, Santa Cruz), RNA polymerase II (SC 900, Santa Cruz) or control IgG was added to cell lysate and incubated at 4℃ overnight. DNA crosslinked with ARID2 was pulled down with Protein G beads and washed and purified with minelute PCR purification kit (NO. 28004, QIANGEN). Aliquots of ChIP-enriched DNA were subjected to sequencing. Libraries were prepared according to Illumina's instructions for ChIP-seq."],"repository":["NODE"],"additional_accession":[]},"is_claimable":false,"name":"ChIP-seq","description":"Arid2 ChIP experiments were performed in K and KL mouse models for sequencing.","dates":{"publication":"2021-12-31","submission":"2019-11-08"},"accession":"OEX00002089","cross_references":{"NODE":["OEP00000654"]}}