NODEGenomicsZhaoyuan FangChIP-Seqhttps://www.biosino.org/node/experiment/detail/OEX00002089Illumina HiSeq 2000PairedChIPY4['lung']['Mus musculus']Cells were cross-linked with 1% formaldehyde for 5 min at room temperature, lysed by SDS Lysis buffer and sonicated to generate DNA fragments with an average size of 500 bp. After pre-clearing with Protein G beads, antibodies against ARID2 (SC 98299X, Santa Cruz), RNA polymerase II (SC 900, Santa Cruz) or control IgG was added to cell lysate and incubated at 4℃ overnight. DNA crosslinked with ARID2 was pulled down with Protein G beads and washed and purified with minelute PCR purification kit (NO. 28004, QIANGEN). Aliquots of ChIP-enriched DNA were subjected to sequencing. Libraries were prepared according to Illumina's instructions for ChIP-seq.NODEfalseChIP-seqArid2 ChIP experiments were performed in K and KL mouse models for sequencing.2021-12-312019-11-08OEX00002089OEP00000654