NODEOtherTing ZhuAMPLICONhttps://www.biosino.org/node/experiment/detail/OEX00002212Illumina HiSeq 2000RANDOMPaired190['soil metagenome']The V4 region of 16S rRNA gene was amplified using the primer pair 515F (5’-GTGYCAGCMGCCGCGGTAA-3’) and 806R (5’-GGACTACNVGGGTWTCTAAT-3’) [1, 2]. A unique 12-bp barcode and a 2-bp linker (5’-TC-3’) were inserted between the barcode and the forward primer (5’-NNNNNNNNNNNNTC-515F-3’) to tag each PCR product. Sequencing was performed on the HiSeq platform (Illumina, San Diego, USA) to generate 250 base-pair paired-end reads.NODEfalse16S rRNA gene amplicon sequencing of saltmarsh soil16S rRNA gene amplicon sequencing of saltmarsh soil2021-09-162020-01-13OEX00002212OEP00000745