NODESingle Cell TranscriptomicsZhang YuRNA-SeqIllumina HiSeq 4000https://www.biosino.org/node/experiment/detail/OEX00010415PairedcDNAN8['skin']['Homo sapiens']Following resection in the operating room, resection specimens were temporarily stored in MACS Tissue Storage Solution (Miltenyi) and rapidly transported to the research facility. On arrival, samples were rinsed with PBS several times to remove as much blood cells as possible. Each sample was stripped of surrounding normal skin tissue, epidermis and excess subcutaneous adipose tissue and subsequently minced to small pieces of less than 1 mm3. Then, small pieces were transferred to digestion medium containing 0.2% Liberase (Roche) and DNAse I (Roche) in DMEM (Gibco). Samples were incubated for 30 min at 37 °C, with gentlely shaking every 5 min. Next, 10 ml PBS containing 2% fetal bovine serum (Gibco) was added and samples were filtered using a 100-μm cell strainers (BD Falcon). Following centrifugation at 120× g and 4 °C for 5 min, the supernatant was decanted and discarded, and the cell pellet was resuspended in 5 ml red blood cell lysis buffer and transferred to a 2-ml DNA low bind tube. Following a 5-min incubation at room temperature, samples were centrifuged (120× g, 4 °C, 5 min) , samples were next resuspended in 1 ml PBS containing 2% fetal bovine serum and filter ed over 40-μm cell strainers (BD Falcon). Then, dead cells in samples were removed by using Dead Cell Removal Kit (Miltenyi) and the remaining cell pellet was resuspended in 1 ml PBS containing 0.04% BSA (Gibco) using wide-bore 1 ml low-retention filter tips (Mettler-Toledo). Next, 10 μl of this cell suspension was counted using an automated cell counter (Thermo Fisher Scientific) by trypanblau test to determine the concentration of live cells. Throughout the dissociation procedure, cells were maintained on ice whenever possible, and the entire procedure was completed in less than 90 min to avoid dissociation associated artefacts recently described.NODEfalseOEX_Zhang_20082019368 untreated patients with mixed or deep type of IH received resection for treatment in our hospital. According to clinical features of tumors and the age of the patient, 8 cases were divided into three groups, 3 cases at proliferative stage (0-3 month, Pro Stage), 3 cases at plateau stage (4-12 month, Plat Stage ) and 2 cases at involuting stage (13 to 36 month, Inv Stage).2020-08-212020-08-20OEX00010415OEP00001116