NODEProteomicsXiaofeng ChenQ Exactive Plushttps://www.biosino.org/node/experiment/detail/OEX0001085011The cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), then were flash-frozen in liquid nitrogen and stored at − 80 °C. For digestion, the protein solution was reduced with 5 mM dithiothreitol for 30 min at 56 °C and alkylated with 11 mM iodoacetamide for 15 min at room temperature in darkness. The protein sample was then diluted by adding 100 mM TEAB to urea concentration less than 2M. Finally, trypsin was added at 1:50 trypsin-to-protein mass ratio for the first digestion overnight and 1:100 trypsin-to-protein mass ratio for a second 4 h-digestion.['Roseobacter denitrificans OCh 114', 'Ruegeria pomeroyi DSS-3', 'Dinoroseobacter shibae DFL 12 = DSM 16493']NODEfalseBacterial proteomicsRoseobacter strains were cultured in modified Silicibacter basal medium, pH ~7.2, added with 10mM DHPS or 15mM acetate, respectively. Cells were harvested via centrifugation at the exponential phase or the stationary phase. A Label-free analyses of total proteins of crude extracts of DHPS- and acetate-grown cells was investigated.2023-03-132020-12-01OEX00010850OEP00001337