{"database":"NODE","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"submitter":["Weiwei Zhang"],"technology_type":["AMPLICON"],"full_dataset_link":["https://www.biosino.org/node/experiment/detail/OEX00012567"],"experiment_platform":["HiSeq X Ten"],"experiment_library_layout":["Paired"],"experiment_library_selection":["PCR"],"experiment_mate_pair":["Y"],"sample_count":["5"],"tissue":["['cell Line']"],"taxonomy":["['Homo sapiens']"],"experiment_protocol":["Generally, biotinylated primer was designed within 150 bp around Cas9-targeting site to achieve primer extension. Site-specific nested primer was designed for following amplification. All the PEM-seq libraries were sequenced by Illumina Hiseq. For off-target analysis, junctions proximal to break-site (±20 kb) were excluded and MACS2 callpeak was used to identify translocation enrich region. Off-target hotspots were defined to have less than 8 mismatches with on-target site and have more than 3 junctions at the presumable cutting site."],"repository":["NODE"],"additional_accession":[]},"is_claimable":false,"name":"PEM-seq","description":"PEM-seq construction and analysis for off-target, translocation and large deletion.","dates":{"publication":"2021-03-12","submission":"2021-03-11"},"accession":"OEX00012567","cross_references":{"NODE":["OEP00001824"]}}