{"database":"NODE","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"submitter":["Jianxiang Shi"],"technology_type":["RNA-Seq"],"experiment_platform":["Illumina NovaSeq 6000"],"full_dataset_link":["https://www.biosino.org/node/experiment/detail/OEX00014374"],"experiment_library_selection":["PolyA"],"experiment_library_layout":["Single"],"sample_count":["1"],"tissue":["['pbmc']"],"taxonomy":["['Homo sapiens']"],"repository":["NODE"],"additional_accession":[]},"is_claimable":false,"name":"OEX_Jianxiang_2108112154","description":"A significant complication of chronic inflammation is anemia, which may be related to disregulated activities of erythroblastic island (EBI) macrophages. GM-CSF was reported to be upregulated and attracted as a therapeutic target in many inflammatory diseases. Among EBI, we found that GM-CSF receptor is expressed preferentially and highly on EBI macrophages but not erythroblasts. GM-CSF treatment significantly decreases the human EBI formation in vitro via decreasing the adhesion molecule expression of CD163. RNA-seq analyses suggest GM-CSF treatment impairs the supporting function of human EBI macrophages during erythropoiesis. GM-CSF treatment also polarizes human EBI macrophages from M2-like type to M1-like type. In addition, GM-CSF decreases mouse bone marrow (BM) erythroblasts as well as EBI macrophages, leading to reduction in EBI numbers. To define molecular mechanism, we found that GM-CSF treatment significantly decreases the adhesion molecule expression of CD163 and Vcam1 in vivo. Importantly, GM-CSF treatment also decreases the phagocytosis rate of EBI macrophages in mouse BM, as well as the expression of engulfment-related molecules, Mertk, Axl, and Timd4. In addition, GM-CSF treatment polarizes mouse BM EBI macrophages from M2-like type to M1-like type. Thus, we have documented that GM-CSF impairs EBI formation in mouse and man. Our findings provide significant resources for anemia of inflammatory diseases and targeting GM-CSF or reprogramming EBI macrophages might be a novel strategy for these diseases.","dates":{"publication":"2021-10-26","submission":"2021-08-11"},"accession":"OEX00014374","cross_references":{"NODE":["OEP00002596"]}}