NODESingle Cell TranscriptomicsZechuan ChenRNA-SeqIllumina NovaSeq 6000https://www.biosino.org/node/experiment/detail/OEX00014785PairedcDNAY1['pbmc']['Homo sapiens']Single cells of different subtypes, including T cells (CD3+ CD19-), B cells (CD3-CD19+), and Plasma cells (CD3-CD19+CD20-CD38hi), were sorted on a FACSAria II using gating strategy shown in sup fig. Cells were filtered using 40μm cell-strainer nylon mesh (Falcon) and stained with DAPI before sorting. Chromium Next GEM Single Cell 5′ Reagent Kits (V2) were used to prepare single cell 5’ gene expression libraries, 5’ cell surface protein libraries and V(D)J libraries following the manufacture’s protocol (10× Genomics).NODEfalseOEP002899_PBMC_genome_BioNanoSingle-cell RNA sequencing of sorted T and B lymphocyte cells in peripheral blood of MDA5+ DM patients and other DM and healthy.2022-06-052021-11-15OEX00014785OEP00002899