{"database":"NODE","file_versions":[],"scores":null,"additional":{"omics_type":["Single Cell Transcriptomics"],"submitter":["Fei Li"],"technology_type":["RNA-Seq"],"experiment_platform":["Illumina NovaSeq 6000"],"full_dataset_link":["https://www.biosino.org/node/experiment/detail/OEX00014925"],"experiment_library_layout":["Paired"],"experiment_library_selection":["PCR"],"sample_count":["7"],"tissue":["['prostate']"],"taxonomy":["['Mus musculus']"],"experiment_protocol":["The prostates of T2PPRC mice were dissected and minced with scissors. The dissociated prostate cells were resuspended in Red Blood Cell Lysis Solution (Miltenyi Biotec, no. 130-094-183) and incubated at room temperature for 2 min. Dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec, no. 130-090-101), and the cells were counted via the Countess II FL Automated Cell Counter (Thermo Fisher Scientific). Cells were lysed on ice for 5 min. Isolated nuclei from T2PPRC mouse prostates cells were transposed and loaded onto the Chromium Next GEM Chip J (10X Genomics). Then, the chip with prostate cells was loaded on a Chromium Controller. The library was constructed according to the manufacturer’s instructions. Fifteen mouse prostate samples with different barcodes were run on a NovaSeq6000 (Illumina) (To overcome the limitation of 4.5 months as the lifespan for TPPRC mice due to probable lesions of other organs, we grafted 4.5-month prostate tumors under renal capsule of SCID mice for another 1.5 months to achieve total time of 6 months)."],"repository":["NODE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell multiomics","description":"Single-cell multiomics sequencing analysis of cells freshly dissociated from prostate tumors with independent replicates at different cancer progression stages (Figures 1B and S1D), which enabled the joint profiling of chromatin accessibility and gene expression in the same individual cell.","dates":{"publication":"2022-08-27","submission":"2021-12-12"},"accession":"OEX00014925","cross_references":{"pubmed":["10.1016/j.ccell.2022.10.011","36332622"],"NODE":["OEP00002972"]}}