{"database":"NODE","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"submitter":["Delong Feng"],"technology_type":["RNA-Seq"],"full_dataset_link":["https://www.biosino.org/node/experiment/detail/OEX00016367"],"experiment_platform":["Illumina HiSeq 2000"],"experiment_library_layout":["Paired"],"experiment_library_selection":["cDNA_oligo_dT"],"sample_count":["6"],"tissue":["['pbmc']"],"taxonomy":["['Homo sapiens']"],"experiment_protocol":["For RNA-seq, The sorted CD4+ T cells were isolated using a TRIzol (Life Technologies). The total RNA was quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) .A total amount of 1 μg RNA per sample was used and mRNA was purified using poly-T oligo-attached magnetic beads. First strand cDNA was synthesized using random hexamer primer. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I. The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). The sequencing was performed by Illumina  Novaseq platform."],"repository":["NODE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of CD4+T cells form SLE patient and healthy control","description":"RNA-seq of CD4+T cells form SLE patient and healthy control","dates":{"publication":"2022-09-19","submission":"2022-03-25"},"accession":"OEX00016367","cross_references":{"NODE":["OEP00003219"]}}