NODETranscriptomicsYu YaoRNA-Seqhttps://www.biosino.org/node/experiment/detail/OEX00016865HiSeq X TenPairedPolyAY20['brain']['Homo sapiens']The fresh GBM tumor tissue from patients undergoing surgical resection were acquired after getting informed written consent. Tumor-derived macrophage/microglia were isolated as mentioned previously. Briefly, samples were initially dissociated into fragments under sterile conditions using scissors. Then, tissue fragments were digested to be suspensions using papain. The suspensions were resuspended in cold sterile PBS and filtered through a 40-μm strainer cap, followed by centrifugation for 5 min at 300 g. CD11b+ cells were sorted using CD11b microbeads (Miltenyi Biotec, 130-093-634, Germany) followed by procedures provided by the manufacturer. Finally, the total RNA of CD11b+ cells were extracted and used for RNA sequencing at illumina platform. The fastq data of sequencing was processed as general pipeline, and the data in FPKM (Fragments Per Kilobase per Million) was generated for further bioinformatic analysis.NODEfalseGMAH_ Macrophage/Microglia_RNA-SeqTumor-infiltrating macrophages/microglia were isolated from GBM tissues and used for RNA sequencing to obtain expression signature of macrophages/microglia. These data were extracted from Glioma Microenvironment Atlas of Huashan (GMAH)2022-05-312022-05-31OEX00016865OEP00003422