NODEGenomicsZengwei TangChIP-Seqhttps://www.biosino.org/node/experiment/detail/OEX00020408Illumina NovaSeq 6000PairedChIP1['human Intrahepatic Cholangiocarcarcinoma Cell Line']['Homo sapiens']HuCCT1 cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, USA), 100μg/ml streptomycin (Cienry, Huzhou, CN), and 100 U/mL penicillin (Cienry, Huzhou, CN) at 37 °C in 5% CO2 atmosphereNODEfalseOEX_Zengwei_2208181103Approximately 1×107 HuCCT1 cells in the logarithmic phase were fixed with 4% paraformaldehyde and incubated with glycine at 37°C for 10 min. After lysis, shearing of the chromatin into 100-600bp fragments by sonication, the lysates were immunoprecipitated with magnetic protein G beads conjugated with anti-MLF1 antibody or used as Input (positive control).2022-08-182022-08-18OEX00020408OEP00003577